Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.105 extracted from

  • Gallego, O.; Belyaeva, O.V.; Porte, S.; Ruiz, F.X.; Stetsenko, A.V.; Shabrova, E.V.; Kostereva, N.V.; Farres, J.; Pares, X.; Kedishvili, N.Y.
    Comparative functional analysis of human medium-chain dehydrogenases, short-chain dehydrogenases/reductases and aldo-keto reductases with retinoids (2006), Biochem. J., 399, 101-109.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
all-trans-retinol 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
all-trans-retinol + NAD+ Homo sapiens RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context all-trans-retinal + NADH
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O75452
-
-

Purification (Commentary)

Purification (Comment) Organism
preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinol + NAD+ RoDH-4 is capable of contributing to the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in the cellular context Homo sapiens all-trans-retinal + NADH
-
?
all-trans-retinol + NAD+
-
Homo sapiens all-trans-retinal + NADH + H+
-
?
additional information the enzymes utilizes retinol bound to cellular retinol binding protein type I at a much lower rate than free retinol Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
RoDH-4
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
all-trans-retinol 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Homo sapiens