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Literature summary for 1.1.1.105 extracted from

  • Lapshina, E.A.; Belyaeva, O.V.; Chumakova, O.V.; Kedishvili, N.Y.
    Differential recognition of the free versus bound retinol by human microsomal retinol/sterol dehydrogenases: characterization of the holo-CRBP dehydrogenase activity of RoDH-4 (2003), Biochemistry, 42, 776-784.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cellular retinol binding protein free form without retinol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
androsterone pH 7.3, 37°C Homo sapiens
0.00024
-
NAD+ pH 7.3, 37°C Homo sapiens
0.0027
-
retinol bound to cellular retinol binding protein pH 7.3, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome integral membrane protein facing cytosolic side of the membrane Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton Homo sapiens

Storage Stability

Storage Stability Organism
4°C, reconstitution of enzyme into proteoliposomes, stable for at least 4 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androsterone + NAD+
-
Homo sapiens ? + NADH
-
?
additional information substrate bound to cellular retinol binding protein is preferred Homo sapiens ?
-
?
retinol + NAD+
-
Homo sapiens retinal + NADH
-
?
retinol bound to cellular retinol binding protein + NAD+
-
Homo sapiens retinal bound to cellular retinol binding protein + NADH
-
?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
RoDH-4
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00003
-
cellular retinol binding protein pH 7.3, 37°C Homo sapiens