BRENDA - Enzyme Database show
show all sequences of 1.1.1.104

Partial purification and some properties of 4-ketoproline reductase

Smith, T.E.; Mitoma, C.; J. Biol. Chem. 237, 1177-1180 (1962)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
4-Oxoproline
-
Oryctolagus cuniculus
0.84
-
NADH
-
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-oxo-proline + NADH
Oryctolagus cuniculus
-
4-hydroxy-L-proline + NAD+
-
Oryctolagus cuniculus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryctolagus cuniculus
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, negative alumina gel, positive alumina gel
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.21
-
-
Oryctolagus cuniculus
Storage Stability
Storage Stability
Organism
-10░C, 160 mM phosphate buffer, pH 6.0, 3 months, no loss of activity
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-oxo-proline + NADH
-
285722
Oryctolagus cuniculus
4-hydroxy-L-proline + NAD+
-
285722
Oryctolagus cuniculus
ir
4-oxoproline + NADH
-
285722
Oryctolagus cuniculus
4-hydroxy-L-proline + NAD+
-
285722
Oryctolagus cuniculus
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
decrease of activity below pH 6.0
Oryctolagus cuniculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Oryctolagus cuniculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.6
-
4-Oxoproline
-
Oryctolagus cuniculus
0.84
-
NADH
-
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-oxo-proline + NADH
Oryctolagus cuniculus
-
4-hydroxy-L-proline + NAD+
-
Oryctolagus cuniculus
ir
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, negative alumina gel, positive alumina gel
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Oryctolagus cuniculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.21
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
Storage Stability
Organism
-10░C, 160 mM phosphate buffer, pH 6.0, 3 months, no loss of activity
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-oxo-proline + NADH
-
285722
Oryctolagus cuniculus
4-hydroxy-L-proline + NAD+
-
285722
Oryctolagus cuniculus
ir
4-oxoproline + NADH
-
285722
Oryctolagus cuniculus
4-hydroxy-L-proline + NAD+
-
285722
Oryctolagus cuniculus
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
decrease of activity below pH 6.0
Oryctolagus cuniculus
Other publictions for EC 1.1.1.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285722
Smith
Partial purification and some ...
Oryctolagus cuniculus
J. Biol. Chem.
237
1177-1180
1962
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