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show all sequences of 1.1.1.102

Tsc10p and FVT1: Topologically distinct short-chain reductases required for long-chain base synthesis in yeast and mammals

Gupta, S.D.; Gable, K.; Han, G.; Borovitskaya, A.; Selby, L.; Dunn, T.M.; Harmon, J.M.; J. Lipid Res. 50, 1630-1640 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
FVT1 expressed in CHO cells or yeast
Homo sapiens
TSC10p expressed in CHO cells or yeast
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
A175T
mutation is deleterious to the human enzyme
Homo sapiens
additional information
mutation of any of the three residues that constitute the catalytic triad of FVT1 do not completely inactivate the enzyme, as it is able to support growth of tsc10DELTA mutant cells at 26C, but not at 37C. Deletion of residues 4-26 from the FVT1-GFP protein abolishes ER localization
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
siRNA
reduces expression of transfected FVT1 mRNA and protein by at least 70%
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
C-terminus of FVT1
Homo sapiens
5737
-
cytoplasm
N- and C-terminus of Tsc10p
Saccharomyces cerevisiae
5737
-
endoplasmic reticulum
N-terminal membrane-spanning domain in FVT1 orients to place it in the endoplasmic reticulum lumen
Homo sapiens
5783
-
endoplasmic reticulum
Tsc10p localizes to the yeast endoplasmic reticulum membrane
Saccharomyces cerevisiae
5783
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration1
Homo sapiens
150000
-
gel filtration
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Saccharomyces cerevisiae
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydrosphinganine + NADPH + H+
-
699413
Homo sapiens
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH + H+
-
699413
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
additional information
FVT1 can replace Tsc10p in yeast
699413
Homo sapiens
?
-
-
-
-
additional information
FVT1 is able to support growth of tsc10DELTA mutant cells at 26C, but not at 37C
699413
Saccharomyces cerevisiae
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
gel filtration
Homo sapiens
tetramer
gel filtration
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
FVT1 expressed in CHO cells or yeast
Homo sapiens
TSC10p expressed in CHO cells or yeast
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A175T
mutation is deleterious to the human enzyme
Homo sapiens
additional information
mutation of any of the three residues that constitute the catalytic triad of FVT1 do not completely inactivate the enzyme, as it is able to support growth of tsc10DELTA mutant cells at 26C, but not at 37C. Deletion of residues 4-26 from the FVT1-GFP protein abolishes ER localization
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
siRNA
reduces expression of transfected FVT1 mRNA and protein by at least 70%
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
C-terminus of FVT1
Homo sapiens
5737
-
cytoplasm
N- and C-terminus of Tsc10p
Saccharomyces cerevisiae
5737
-
endoplasmic reticulum
N-terminal membrane-spanning domain in FVT1 orients to place it in the endoplasmic reticulum lumen
Homo sapiens
5783
-
endoplasmic reticulum
Tsc10p localizes to the yeast endoplasmic reticulum membrane
Saccharomyces cerevisiae
5783
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
150000
-
gel filtration1
Homo sapiens
150000
-
gel filtration
Saccharomyces cerevisiae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydrosphinganine + NADPH + H+
-
699413
Homo sapiens
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH + H+
-
699413
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
additional information
FVT1 can replace Tsc10p in yeast
699413
Homo sapiens
?
-
-
-
-
additional information
FVT1 is able to support growth of tsc10DELTA mutant cells at 26C, but not at 37C
699413
Saccharomyces cerevisiae
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
gel filtration
Homo sapiens
tetramer
gel filtration
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721947
Timmins-Schiffman
Characterization of genes invo ...
Crassostrea gigas
BMC Res. Notes
5
502
2012
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1
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6
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1
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1
1
1
1
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721891
Kim
-
Effects of expression of lcb1/ ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae KCCM 50515
Biotechnol. Bioprocess Eng.
16
1-6
2011
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1
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2
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2
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723408
Chao
Sphingolipids in the root play ...
Arabidopsis thaliana
Plant Cell
23
1061-1081
2011
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1
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1
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2
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5
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2
2
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699413
Gupta
Tsc10p and FVT1: Topologically ...
Homo sapiens, Saccharomyces cerevisiae
J. Lipid Res.
50
1630-1640
2009
-
-
2
-
2
-
1
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4
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2
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5
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1
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4
2
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4
2
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689780
Krebs
A missense mutation in the 3-k ...
Bos taurus
Proc. Natl. Acad. Sci. USA
104
6746-6751
2007
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1
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1
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6
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1
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667831
Fornarotto
Sphingolipid biosynthesis in p ...
Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1761
52-63
2006
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3
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2
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2
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3
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7
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1
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3
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1
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3
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656256
Kihara
FVT-1 is a mammalian 3-ketodih ...
Homo sapiens, Mus musculus
J. Biol. Chem.
279
49243-49250
2004
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2
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2
4
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6
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13
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285697
Beeler
The Saccharomyces cerevisiae T ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
30688-30694
1998
1
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285698
Mandon
Subcellular localization and m ...
Mus musculus
J. Biol. Chem.
267
11144-11148
1992
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285699
Stoffel
Stereospecificity of the NADPH ...
Bos taurus, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
1637-1644
1968
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285700
Stoffel
Biosynthesis of dihydrosphingo ...
Pichia ciferrii, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
664-670
1968
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