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show all sequences of 1.1.1.102

Sphingolipid biosynthesis in pathogenic fungi: identification and characterization of the 3-ketosphinganine reductase activity of Candida albicans and Aspergillus fumigatus

Fornarotto, M.; Xiao, L.; Hou, Y.; Koch, K.A.; Chang, E.; OMalley, R.M.; Black, T.A.; Cable, M.B.; Walker, S.S.; Biochim. Biophys. Acta 1761, 52-63 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
Candida albicans
gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
Aspergillus fumigatus
gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of an enzyme-deficient mutant by gene replacement, the mutant produces lower levels of inositolphosphorylceramides
Candida albicans
additional information
construction of the deletion mutant TscDELTA38p
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
binding kinetics of substrate analogues with shorter chain lengths
Candida albicans
0.009
-
3-dehydrosphinganine
pH 7.5, recombinant enzyme
Candida albicans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-dehydrosphinganine + NADPH
Aspergillus fumigatus
-
sphinganine + NADP+
-
-
?
3-dehydrosphinganine + NADPH
Saccharomyces cerevisiae
the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview
sphinganine + NADP+
-
-
?
3-dehydrosphinganine + NADPH
Candida albicans
the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides
sphinganine + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
gene ksrA
-
Candida albicans
-
gene ksr1
-
Saccharomyces cerevisiae
-
strain S288c
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydrosphinganine + NADPH
-
667831
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
-
667831
Aspergillus fumigatus
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview
667831
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides
667831
Candida albicans
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
C18 substrate, molecular modeling of substrate binding, overview
667831
Candida albicans
sphinganine + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview
Candida albicans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.07
-
3-dehydrosphinganine
pH 7.5, recombinant enzyme
Candida albicans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus fumigatus
7.5
-
assay at
Candida albicans
7.5
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus fumigatus
NADPH
-
Candida albicans
NADPH
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ksr1, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
Candida albicans
gene ksrA, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain JM109(DE3)
Aspergillus fumigatus
gene tsc10, expression of mutant tscDELTA38 in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Aspergillus fumigatus
NADPH
-
Candida albicans
NADPH
-
Saccharomyces cerevisiae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of an enzyme-deficient mutant by gene replacement, the mutant produces lower levels of inositolphosphorylceramides
Candida albicans
additional information
construction of the deletion mutant TscDELTA38p
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
binding kinetics of substrate analogues with shorter chain lengths
Candida albicans
0.009
-
3-dehydrosphinganine
pH 7.5, recombinant enzyme
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-dehydrosphinganine + NADPH
Aspergillus fumigatus
-
sphinganine + NADP+
-
-
?
3-dehydrosphinganine + NADPH
Saccharomyces cerevisiae
the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview
sphinganine + NADP+
-
-
?
3-dehydrosphinganine + NADPH
Candida albicans
the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides
sphinganine + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-dehydrosphinganine + NADPH
-
667831
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
-
667831
Aspergillus fumigatus
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
the enzyme catalyzes an early step in the sphingolipid biosynthesis, overview
667831
Saccharomyces cerevisiae
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
the enzyme is not essential for cell viability, but enzyme-deficient mutants produce lower levels of inositolphosphorylceramides
667831
Candida albicans
sphinganine + NADP+
-
-
-
?
3-dehydrosphinganine + NADPH
C18 substrate, molecular modeling of substrate binding, overview
667831
Candida albicans
sphinganine + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
molecular modeling of the enzyme with bound substrates, a significant portion of the aliphatic chain of 3-dehydrosphinganine protrudes from the enzyme, overview
Candida albicans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.07
-
3-dehydrosphinganine
pH 7.5, recombinant enzyme
Candida albicans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus fumigatus
7.5
-
assay at
Candida albicans
7.5
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721947
Timmins-Schiffman
Characterization of genes invo ...
Crassostrea gigas
BMC Res. Notes
5
502
2012
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1
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1
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6
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1
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1
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1
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1
1
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1
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1
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1
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-
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1
1
1
1
-
-
721891
Kim
-
Effects of expression of lcb1/ ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae KCCM 50515
Biotechnol. Bioprocess Eng.
16
1-6
2011
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-
-
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1
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2
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2
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2
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1
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1
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1
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2
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2
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723408
Chao
Sphingolipids in the root play ...
Arabidopsis thaliana
Plant Cell
23
1061-1081
2011
-
-
1
-
1
-
-
-
2
-
-
1
-
5
-
-
-
-
-
2
-
-
2
-
-
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1
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1
1
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1
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2
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-
1
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-
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-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
699413
Gupta
Tsc10p and FVT1: Topologically ...
Homo sapiens, Saccharomyces cerevisiae
J. Lipid Res.
50
1630-1640
2009
-
-
2
-
2
-
1
-
4
-
2
-
-
5
-
-
-
-
-
1
-
-
4
2
-
-
-
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2
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2
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1
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4
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2
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1
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4
2
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-
-
-
-
-
-
-
-
-
-
689780
Krebs
A missense mutation in the 3-k ...
Bos taurus
Proc. Natl. Acad. Sci. USA
104
6746-6751
2007
-
1
-
-
1
-
-
-
-
-
-
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6
-
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-
1
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1
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1
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1
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
667831
Fornarotto
Sphingolipid biosynthesis in p ...
Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1761
52-63
2006
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3
-
2
-
-
2
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3
-
7
-
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-
-
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-
-
5
1
-
-
-
1
3
-
-
3
-
-
-
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-
3
3
-
2
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-
-
2
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3
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-
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-
5
1
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-
-
1
3
-
-
-
-
-
-
-
-
-
656256
Kihara
FVT-1 is a mammalian 3-ketodih ...
Homo sapiens, Mus musculus
J. Biol. Chem.
279
49243-49250
2004
-
-
2
-
-
-
-
2
4
-
-
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6
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13
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2
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2
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2
2
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2
4
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13
-
-
2
-
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-
-
-
-
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-
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-
-
-
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-
285697
Beeler
The Saccharomyces cerevisiae T ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
30688-30694
1998
1
-
1
-
-
-
-
-
-
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1
-
3
-
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1
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-
-
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2
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1
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1
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1
1
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1
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1
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2
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285698
Mandon
Subcellular localization and m ...
Mus musculus
J. Biol. Chem.
267
11144-11148
1992
-
-
-
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-
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1
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2
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2
1
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2
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1
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1
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1
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2
1
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2
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-
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-
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-
-
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285699
Stoffel
Stereospecificity of the NADPH ...
Bos taurus, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
1637-1644
1968
-
-
-
-
-
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2
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2
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1
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8
1
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2
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1
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2
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2
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2
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1
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8
1
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2
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1
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-
285700
Stoffel
Biosynthesis of dihydrosphingo ...
Pichia ciferrii, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
664-670
1968
-
-
-
-
-
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2
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1
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