BRENDA - Enzyme Database show
show all sequences of 1.1.1.102

FVT-1 is a mammalian 3-ketodihydrosphingosine reductase with an active site that faces the cytosolic side of the endoplasmic reticulum membrane

Kihara, A.; Igarashi, Y.; J. Biol. Chem. 279, 49243-49250 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in yeast cells
Homo sapiens
expression in yeast cells
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
3-ketodihydrosphingosine
pH 7.5, 37°C
Homo sapiens
0.028
-
NADPH
pH 7.5, 37°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
endoplasmic reticulum
-
Mus musculus
5783
-
membrane
of endoplasmic reticulum, the large hydrophilic domain, which contains putative active site residues, faces the cytosol
Homo sapiens
16020
-
membrane
of endoplasmic reticulum
Mus musculus
16020
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q06136
-
-
Mus musculus
Q6GV12
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
colon
low expression
Homo sapiens
-
heart
high expression
Homo sapiens
-
heart
low level of expression
Mus musculus
-
kidney
high expression
Mus musculus
-
leukocyte
pheripheral blood, low expression
Homo sapiens
-
lung
high expression
Mus musculus
-
placenta
highest expression
Mus musculus
-
skeletal muscle
high expression
Homo sapiens
-
skeletal muscle
low level of expression
Mus musculus
-
small intestine
high expression
Mus musculus
-
spleen
low level of expression
Mus musculus
-
stomach
high expression
Mus musculus
-
thymus
low expression
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-ketodihydrosphingosine + NADPH
-
656256
Homo sapiens
dihydrosphingosine + NADP+
-
-
-
?
3-ketodihydrosphingosine + NADPH
-
656256
Mus musculus
dihydrosphingosine + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in yeast cells
Homo sapiens
expression in yeast cells
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
NADPH
-
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.003
-
3-ketodihydrosphingosine
pH 7.5, 37°C
Homo sapiens
0.028
-
NADPH
pH 7.5, 37°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
endoplasmic reticulum
-
Mus musculus
5783
-
membrane
of endoplasmic reticulum, the large hydrophilic domain, which contains putative active site residues, faces the cytosol
Homo sapiens
16020
-
membrane
of endoplasmic reticulum
Mus musculus
16020
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
colon
low expression
Homo sapiens
-
heart
high expression
Homo sapiens
-
heart
low level of expression
Mus musculus
-
kidney
high expression
Mus musculus
-
leukocyte
pheripheral blood, low expression
Homo sapiens
-
lung
high expression
Mus musculus
-
placenta
highest expression
Mus musculus
-
skeletal muscle
high expression
Homo sapiens
-
skeletal muscle
low level of expression
Mus musculus
-
small intestine
high expression
Mus musculus
-
spleen
low level of expression
Mus musculus
-
stomach
high expression
Mus musculus
-
thymus
low expression
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-ketodihydrosphingosine + NADPH
-
656256
Homo sapiens
dihydrosphingosine + NADP+
-
-
-
?
3-ketodihydrosphingosine + NADPH
-
656256
Mus musculus
dihydrosphingosine + NADP+
-
-
-
?
Other publictions for EC 1.1.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721947
Timmins-Schiffman
Characterization of genes invo ...
Crassostrea gigas
BMC Res. Notes
5
502
2012
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1
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1
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6
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1
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1
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1
1
1
1
-
-
721891
Kim
-
Effects of expression of lcb1/ ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae KCCM 50515
Biotechnol. Bioprocess Eng.
16
1-6
2011
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-
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1
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2
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1
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2
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723408
Chao
Sphingolipids in the root play ...
Arabidopsis thaliana
Plant Cell
23
1061-1081
2011
-
-
1
-
1
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2
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1
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5
-
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2
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2
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2
2
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699413
Gupta
Tsc10p and FVT1: Topologically ...
Homo sapiens, Saccharomyces cerevisiae
J. Lipid Res.
50
1630-1640
2009
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2
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2
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1
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4
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2
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5
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1
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4
2
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4
2
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689780
Krebs
A missense mutation in the 3-k ...
Bos taurus
Proc. Natl. Acad. Sci. USA
104
6746-6751
2007
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1
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1
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6
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1
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667831
Fornarotto
Sphingolipid biosynthesis in p ...
Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1761
52-63
2006
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3
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2
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2
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3
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7
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5
1
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1
3
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3
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3
3
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2
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2
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3
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5
1
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1
3
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656256
Kihara
FVT-1 is a mammalian 3-ketodih ...
Homo sapiens, Mus musculus
J. Biol. Chem.
279
49243-49250
2004
-
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2
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2
4
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6
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13
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2
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2
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2
2
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2
4
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13
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2
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285697
Beeler
The Saccharomyces cerevisiae T ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
30688-30694
1998
1
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1
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3
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285698
Mandon
Subcellular localization and m ...
Mus musculus
J. Biol. Chem.
267
11144-11148
1992
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1
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285699
Stoffel
Stereospecificity of the NADPH ...
Bos taurus, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
1637-1644
1968
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1
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285700
Stoffel
Biosynthesis of dihydrosphingo ...
Pichia ciferrii, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
664-670
1968
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