BRENDA - Enzyme Database show
show all sequences of 1.1.1.102

The Saccharomyces cerevisiae TSC10/BR265w gene encoding 3-ketosphinganine reductase is identified in a screen for temperature-sensitive suppressors of the Ca2+-sensitive csg2delta mutant

Beeler, T.; Bacikova, D.; Gable, K.; Hopkins, L.; Johnson, C.; Slife, H.; Dunn, T.; J. Biol. Chem. 273, 30688-30694 (1998)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
tergitol
0.2%, 10fold activation of 2-amino-1-hydroxyoctadecane-3-one reduction
Saccharomyces cerevisiae
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli, Myc-His-tagged enzyme
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-amino-1-hydroxyoctadecane-3-one + NADPH
Saccharomyces cerevisiae
-
2-amino-1,3-dihydroxyoctadecane + NADP+
-
Saccharomyces cerevisiae
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant Myc-His-tagged enzyme, Ni-NTA agarose
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-1-hydroxyoctadecane-3-one + NADPH
-
285697
Saccharomyces cerevisiae
2-amino-1-(R)-3-dihydroxyoctadecane + NADP+
-
285697
Saccharomyces cerevisiae
?
2-amino-1-hydroxyoctadecane-3-one + NADPH
-
285697
Saccharomyces cerevisiae
2-amino-1,3-dihydroxyoctadecane + NADP+
-
285697
Saccharomyces cerevisiae
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
tergitol
0.2%, 10fold activation of 2-amino-1-hydroxyoctadecane-3-one reduction
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli, Myc-His-tagged enzyme
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-amino-1-hydroxyoctadecane-3-one + NADPH
Saccharomyces cerevisiae
-
2-amino-1,3-dihydroxyoctadecane + NADP+
-
Saccharomyces cerevisiae
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant Myc-His-tagged enzyme, Ni-NTA agarose
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-1-hydroxyoctadecane-3-one + NADPH
-
285697
Saccharomyces cerevisiae
2-amino-1-(R)-3-dihydroxyoctadecane + NADP+
-
285697
Saccharomyces cerevisiae
?
2-amino-1-hydroxyoctadecane-3-one + NADPH
-
285697
Saccharomyces cerevisiae
2-amino-1,3-dihydroxyoctadecane + NADP+
-
285697
Saccharomyces cerevisiae
?
Other publictions for EC 1.1.1.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721947
Timmins-Schiffman
Characterization of genes invo ...
Crassostrea gigas
BMC Res. Notes
5
502
2012
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1
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1
1
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1
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721891
Kim
-
Effects of expression of lcb1/ ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae KCCM 50515
Biotechnol. Bioprocess Eng.
16
1-6
2011
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1
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2
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723408
Chao
Sphingolipids in the root play ...
Arabidopsis thaliana
Plant Cell
23
1061-1081
2011
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1
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1
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5
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2
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699413
Gupta
Tsc10p and FVT1: Topologically ...
Homo sapiens, Saccharomyces cerevisiae
J. Lipid Res.
50
1630-1640
2009
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2
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2
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1
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4
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2
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2
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689780
Krebs
A missense mutation in the 3-k ...
Bos taurus
Proc. Natl. Acad. Sci. USA
104
6746-6751
2007
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1
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6
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667831
Fornarotto
Sphingolipid biosynthesis in p ...
Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae
Biochim. Biophys. Acta
1761
52-63
2006
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3
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7
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3
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656256
Kihara
FVT-1 is a mammalian 3-ketodih ...
Homo sapiens, Mus musculus
J. Biol. Chem.
279
49243-49250
2004
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2
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2
4
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6
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2
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13
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2
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285697
Beeler
The Saccharomyces cerevisiae T ...
Saccharomyces cerevisiae
J. Biol. Chem.
273
30688-30694
1998
1
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1
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1
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3
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285698
Mandon
Subcellular localization and m ...
Mus musculus
J. Biol. Chem.
267
11144-11148
1992
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285699
Stoffel
Stereospecificity of the NADPH ...
Bos taurus, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
1637-1644
1968
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285700
Stoffel
Biosynthesis of dihydrosphingo ...
Pichia ciferrii, Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
349
664-670
1968
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