BRENDA - Enzyme Database show
show all sequences of 1.1.1.101

Purification and properties of acyl/alkyl dihydroxyacetone-phosphate reductase from guinea pig liver peroxisomes

Datta, S.C.; Ghosh, M.K.; Hajra, A.K.; J. Biol. Chem. 265, 8268-8274 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
phosphatidylglycerol
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
Triton X-100
destabilizes during solubilization
Cavia porcellus
General Stability
General Stability
Organism
DTT stabilizes during storage
Cavia porcellus
freeze-thawing leads to rapid loss of activity
Cavia porcellus
high ionic strength facilitates solubilization with neutral or anionic detergents
Cavia porcellus
NADPH stabilizes during storage and solubilization
Cavia porcellus
Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present
Cavia porcellus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
stable to N-ethylmaleimide
Cavia porcellus
N-ethylmaleimide
not inhibitory
Cavia porcellus
NADP+
-
Cavia porcellus
palmitoyl-CoA
competitive to NADPH, not to alkyldihydroxyacetone phosphate
Cavia porcellus
Palmitoyldihydroxyacetone phosphate
competitive to hexadecyldihydroxyacetone phosphate
Cavia porcellus
PCMB
-
Cavia porcellus
phosphatidylcholine
at high concentrations
Cavia porcellus
phosphatidylethanolamine
-
Cavia porcellus
phosphatidylglycerol
at high concentrations
Cavia porcellus
phosphatidylinositol
-
Cavia porcellus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0154
-
Palmitoyldihydroxyacetone phosphate
-
Cavia porcellus
0.02
-
NADPH
-
Cavia porcellus
0.021
-
Hexadecyldihydroxyacetone phosphate
-
Cavia porcellus
1.7
-
NADH
-
Cavia porcellus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Cavia porcellus
70000
75000
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
Organic Solvent Stability
Organic Solvent
Commentary
Organism
Glycerol
stabilizes
Cavia porcellus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cavia porcellus
-
-
-
Purification (Commentary)
Commentary
Organism
affinity chromatography on NADPH-agarose gel matrix
Cavia porcellus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62.5
-
-
Cavia porcellus
Storage Stability
Storage Stability
Organism
4°C, stable in the presence of DTT and NADPH, 1 month
Cavia porcellus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285690
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Cavia porcellus
More
27% hydrophobic amino acids by amino acid analysis
Cavia porcellus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
100
-
NADPH
-
Cavia porcellus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
can substitute for NADPH
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
phosphatidylcholine
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
phosphatidylglycerol
micelles, activation, in presence of Triton X-100, slight stimulation at low concentrations
Cavia porcellus
Triton X-100
destabilizes during solubilization
Cavia porcellus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
can substitute for NADPH
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates
Cavia porcellus
General Stability (protein specific)
General Stability
Organism
DTT stabilizes during storage
Cavia porcellus
freeze-thawing leads to rapid loss of activity
Cavia porcellus
high ionic strength facilitates solubilization with neutral or anionic detergents
Cavia porcellus
NADPH stabilizes during storage and solubilization
Cavia porcellus
Triton X-100, enzyme becomes unstable during solubilization unless NADPH and 1 M KCl are present
Cavia porcellus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
stable to N-ethylmaleimide
Cavia porcellus
N-ethylmaleimide
not inhibitory
Cavia porcellus
NADP+
-
Cavia porcellus
palmitoyl-CoA
competitive to NADPH, not to alkyldihydroxyacetone phosphate
Cavia porcellus
Palmitoyldihydroxyacetone phosphate
competitive to hexadecyldihydroxyacetone phosphate
Cavia porcellus
PCMB
-
Cavia porcellus
phosphatidylcholine
at high concentrations
Cavia porcellus
phosphatidylethanolamine
-
Cavia porcellus
phosphatidylglycerol
at high concentrations
Cavia porcellus
phosphatidylinositol
-
Cavia porcellus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0154
-
Palmitoyldihydroxyacetone phosphate
-
Cavia porcellus
0.02
-
NADPH
-
Cavia porcellus
0.021
-
Hexadecyldihydroxyacetone phosphate
-
Cavia porcellus
1.7
-
NADH
-
Cavia porcellus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Cavia porcellus
70000
75000
gel filtration
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
Glycerol
stabilizes
Cavia porcellus
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography on NADPH-agarose gel matrix
Cavia porcellus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62.5
-
-
Cavia porcellus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, stable in the presence of DTT and NADPH, 1 month
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
enzyme links acyldihydroxyacetone phosphate pathway intermediates to cellular glycerolipid biosynthesis
285690
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285690
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285690
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285690
Cavia porcellus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Cavia porcellus
More
27% hydrophobic amino acids by amino acid analysis
Cavia porcellus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
100
-
NADPH
-
Cavia porcellus
Other publictions for EC 1.1.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285695
Athenstaedt
1-Acyldihydroxyacetone-phospha ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
235-240
2000
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
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2
-
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-
-
-
-
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1
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1
1
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1
1
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-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285696
Hajra
Induction of the peroxisomal g ...
Mus musculus
J. Biol. Chem.
275
9441-9446
2000
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
1
-
4
-
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-
-
-
-
-
-
1
-
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-
-
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-
1
-
-
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-
-
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-
-
-
2
-
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-
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-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285694
Athenstaedt
Redundant systems of phosphati ...
Saccharomyces cerevisiae
J. Bacteriol.
181
1458-1463
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
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-
-
-
-
-
2
-
-
-
-
-
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-
1
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-
1
-
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-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285693
Minskoff
Regulation of phosphatidic aci ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae tpa1
J. Lipid Res.
35
2254-2262
1994
4
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
1
-
8
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285691
Racenis
-
The acyl dihydroxyacetone phos ...
Saccharomyces cerevisiae
J. Bacteriol.
174
5704-5710
1992
-
-
-
-
-
-
4
2
-
-
-
4
-
1
-
-
-
-
-
-
-
2
8
-
1
1
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
-
4
-
-
-
-
-
-
-
2
8
-
1
1
2
-
1
-
-
-
-
-
-
-
-
-
285692
Hajra
Acyl/alkyldihydroxyacetone pho ...
Cavia porcellus
Methods Enzymol.
209
402-407
1992
-
-
-
-
-
2
-
4
-
-
2
-
-
1
-
-
1
-
-
1
1
-
4
1
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
2
-
-
-
4
-
-
2
-
-
-
-
1
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285690
Datta
Purification and properties of ...
Cavia porcellus
J. Biol. Chem.
265
8268-8274
1990
3
-
-
-
-
5
10
4
1
-
2
2
1
2
-
-
1
-
-
2
1
1
6
2
-
-
-
1
-
-
-
2
-
-
-
3
-
-
2
-
-
5
-
10
-
4
1
-
2
2
1
-
-
1
-
2
1
1
6
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
285689
Webber
Properties of the enzymes cata ...
Homo sapiens
Arch. Biochem. Biophys.
254
611-620
1987
-
-
-
-
-
-
-
2
-
-
-
2
-
3
-
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-
-
1
-
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5
-
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1
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1
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1
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2
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2
-
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1
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
285688
Ghosh
Subcellular distribution and p ...
Cavia porcellus, Mus musculus, Rattus norvegicus
Arch. Biochem. Biophys.
245
523-530
1986
1
-
-
-
-
6
6
9
2
-
-
6
-
8
-
-
1
-
-
5
2
-
16
-
-
-
1
-
1
1
-
5
-
-
-
1
-
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5
-
-
6
-
6
-
9
2
-
-
6
-
-
-
1
-
5
2
-
16
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
285687
Opperdoes
Localization of the initial st ...
Trypanosoma brucei
FEBS Lett.
169
35-39
1984
-
-
-
-
-
-
-
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-
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2
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4
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1
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1
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4
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-
-
-
-
-
285686
Ballas
Topography of glycerolipid syn ...
Rattus norvegicus, Rattus norvegicus Charles River CD
Biochim. Biophys. Acta
665
586-595
1981
1
-
-
-
-
2
2
-
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-
5
-
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-
1
1
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8
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1
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1
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1
-
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2
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2
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1
1
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8
-
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-
-
-
-
285685
Hajra
Subcellular localization of ac ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
254
10896-10900
1979
-
-
-
-
-
-
-
-
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3
-
162
-
-
-
-
-
1
1
-
11
-
-
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1
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-
1
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-
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-
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3
-
-
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1
1
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11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285684
LaBelle
Enzymatic reduction of alkyl a ...
Cavia porcellus, Rattus norvegicus
J. Biol. Chem.
247
5825-5834
1972
2
-
-
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2
-
5
-
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4
-
2
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12
-
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12
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2
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2
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4
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2
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4
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2
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5
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4
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12
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12
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2
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2
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