BRENDA - Enzyme Database show
show all sequences of 1.1.1.101

Enzymatic reduction of alkyl and acyl derivatives of dihydroxyacetone phosphate by reduced pyridine nucleotides

LaBelle, E.F.; Hajra, A.K.; J. Biol. Chem. 247, 5825-5834 (1972)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Cavia porcellus
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Rattus norvegicus
General Stability
General Stability
Organism
freeze-thawing, stable after several cycles
Cavia porcellus
freeze-thawing, stable after several cycles
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
cosubstrate hexadecyldihydroxyacetone phosphate, brain
Rattus norvegicus
0.007
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, brain
Rattus norvegicus
0.01
-
NADPH
ascites microsomes
Cavia porcellus
0.01
-
NADPH
ascites microsomes
Rattus norvegicus
0.05
-
NADPH
liver mitochondria
Cavia porcellus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cavia porcellus
-
-
-
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adipose tissue
-
Rattus norvegicus
-
ascites tumor cell
grown in the abdomen of swiss white mice
Cavia porcellus
-
ascites tumor cell
grown in the abdomen of swiss white mice
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver
-
Cavia porcellus
-
lung
-
Cavia porcellus
-
spleen
-
Cavia porcellus
-
testis
-
Cavia porcellus
-
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
complete inactivation after 10 min
Cavia porcellus
100
-
complete inactivation after 10 min
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
broad optimum
Rattus norvegicus
6.5
7.5
broad optimum
Cavia porcellus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
at high concentrations, rat liver
Rattus norvegicus
NADH
-
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Cavia porcellus
EDTA
activation, acyldihydroxyacetone phosphate as substrate
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
at high concentrations, rat liver
Rattus norvegicus
NADH
-
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Cavia porcellus
NADPH
hydrogen from B-side of nicotinamide ring is transferred to reduce keto substrates; specific for NADPH
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
freeze-thawing, stable after several cycles
Cavia porcellus
freeze-thawing, stable after several cycles
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
cosubstrate hexadecyldihydroxyacetone phosphate, brain
Rattus norvegicus
0.007
-
NADPH
cosubstrate palmitoyldihydroxyacetone phosphate, brain
Rattus norvegicus
0.01
-
NADPH
ascites microsomes
Cavia porcellus
0.01
-
NADPH
ascites microsomes
Rattus norvegicus
0.05
-
NADPH
liver mitochondria
Cavia porcellus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acyldihydroxyacetone phosphate + NADPH
Cavia porcellus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
Rattus norvegicus
reaction in non-ether lipids biosynthesis
1-acyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Cavia porcellus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
Rattus norvegicus
reaction in ether lipids biosynthesis
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
Rattus norvegicus
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adipose tissue
-
Rattus norvegicus
-
ascites tumor cell
grown in the abdomen of swiss white mice
Cavia porcellus
-
ascites tumor cell
grown in the abdomen of swiss white mice
Rattus norvegicus
-
brain
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver
-
Cavia porcellus
-
lung
-
Cavia porcellus
-
spleen
-
Cavia porcellus
-
testis
-
Cavia porcellus
-
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Cavia porcellus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
acyldihydroxyacetone phosphate + NADPH
reaction in non-ether lipids biosynthesis
285684
Rattus norvegicus
1-acyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
-
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Cavia porcellus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
alkyldihydroxyacetone phosphate + NADPH + H+
reaction in ether lipids biosynthesis
285684
Rattus norvegicus
1-alkyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Cavia porcellus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
hexadecyldihydroxyacetone phosphate + NADPH
-
285684
Rattus norvegicus
1-hexadecyl-sn-glycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Cavia porcellus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Cavia porcellus
?
palmitoylglycerone phosphate + NADPH
i.e. palmitoyldihydroxyacetone phosphate
285684
Rattus norvegicus
1-palmitoylglycerol 3-phosphate + NADP+
-
285684
Rattus norvegicus
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
complete inactivation after 10 min
Cavia porcellus
100
-
complete inactivation after 10 min
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
broad optimum
Rattus norvegicus
6.5
7.5
broad optimum
Cavia porcellus
Other publictions for EC 1.1.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
285695
Athenstaedt
1-Acyldihydroxyacetone-phospha ...
Saccharomyces cerevisiae
J. Biol. Chem.
275
235-240
2000
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285696
Hajra
Induction of the peroxisomal g ...
Mus musculus
J. Biol. Chem.
275
9441-9446
2000
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285694
Athenstaedt
Redundant systems of phosphati ...
Saccharomyces cerevisiae
J. Bacteriol.
181
1458-1463
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285693
Minskoff
Regulation of phosphatidic aci ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae tpa1
J. Lipid Res.
35
2254-2262
1994
4
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
1
-
8
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
1
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285691
Racenis
-
The acyl dihydroxyacetone phos ...
Saccharomyces cerevisiae
J. Bacteriol.
174
5704-5710
1992
-
-
-
-
-
-
4
2
-
-
-
4
-
1
-
-
-
-
-
-
-
2
8
-
1
1
2
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
-
4
-
-
-
-
-
-
-
2
8
-
1
1
2
-
1
-
-
-
-
-
-
-
-
-
285692
Hajra
Acyl/alkyldihydroxyacetone pho ...
Cavia porcellus
Methods Enzymol.
209
402-407
1992
-
-
-
-
-
2
-
4
-
-
2
-
-
1
-
-
1
-
-
1
1
-
4
1
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
2
-
-
-
4
-
-
2
-
-
-
-
1
-
1
1
-
4
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
285690
Datta
Purification and properties of ...
Cavia porcellus
J. Biol. Chem.
265
8268-8274
1990
3
-
-
-
-
5
10
4
1
-
2
2
1
2
-
-
1
-
-
2
1
1
6
2
-
-
-
1
-
-
-
2
-
-
-
3
-
-
2
-
-
5
-
10
-
4
1
-
2
2
1
-
-
1
-
2
1
1
6
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
285689
Webber
Properties of the enzymes cata ...
Homo sapiens
Arch. Biochem. Biophys.
254
611-620
1987
-
-
-
-
-
-
-
2
-
-
-
2
-
3
-
-
-
-
-
1
-
-
5
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
-
-
5
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
285688
Ghosh
Subcellular distribution and p ...
Cavia porcellus, Mus musculus, Rattus norvegicus
Arch. Biochem. Biophys.
245
523-530
1986
1
-
-
-
-
6
6
9
2
-
-
6
-
8
-
-
1
-
-
5
2
-
16
-
-
-
1
-
1
1
-
5
-
-
-
1
-
-
5
-
-
6
-
6
-
9
2
-
-
6
-
-
-
1
-
5
2
-
16
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
285687
Opperdoes
Localization of the initial st ...
Trypanosoma brucei
FEBS Lett.
169
35-39
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
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-
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4
-
-
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-
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285686
Ballas
Topography of glycerolipid syn ...
Rattus norvegicus, Rattus norvegicus Charles River CD
Biochim. Biophys. Acta
665
586-595
1981
1
-
-
-
-
2
2
-
-
-
-
-
-
5
-
-
-
-
-
1
1
-
8
-
-
-
-
-
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-
1
-
-
-
1
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
1
1
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285685
Hajra
Subcellular localization of ac ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
J. Biol. Chem.
254
10896-10900
1979
-
-
-
-
-
-
-
-
-
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3
-
162
-
-
-
-
-
1
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
1
-
11
-
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285684
LaBelle
Enzymatic reduction of alkyl a ...
Cavia porcellus, Rattus norvegicus
J. Biol. Chem.
247
5825-5834
1972
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