Application | Comment | Organism |
---|---|---|
synthesis | the microalga Chlorella sorokiniana and provide a target for genetic engineering to improve D-xylose utilization for microalgal lipid production | Chlorella sorokiniana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | Chlorella sorokiniana | - |
L-xylulose + NADPH + H+ | - |
? | |
xylitol + NADP+ | Chlorella sorokiniana UTEX 1602 | - |
L-xylulose + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella sorokiniana | - |
- |
- |
Chlorella sorokiniana UTEX 1602 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Chlorella sorokiniana | - |
additional information | D-xylose utilization by the oleaginous microalga Chlorella sorokiniana | Chlorella sorokiniana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.026 | - |
crude enzyme extract, induced cells, pH 8.0, 40°C | Chlorella sorokiniana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | - |
Chlorella sorokiniana | L-xylulose + NADPH + H+ | - |
? | |
xylitol + NADP+ | - |
Chlorella sorokiniana UTEX 1602 | L-xylulose + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADP+-linked xylitol dehydrogenase | - |
Chlorella sorokiniana |
XDH | - |
Chlorella sorokiniana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Chlorella sorokiniana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 40% of maximal activity at 40°C, 7% at 70°C, and maximal activity at 50°C | Chlorella sorokiniana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Chlorella sorokiniana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 92% of maximal activity at pH 6.0, 70% at pH 8.0, 60% at pH 9.0, and maximal activity at pH 8.0 | Chlorella sorokiniana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+ | Chlorella sorokiniana | |
NADP+ | specific for | Chlorella sorokiniana |
Organism | Comment | Expression |
---|---|---|
Chlorella sorokiniana | D-xylose uptake induces the enzyme, together with the D-xylose reductase, EC 1.1.1.307 | up |
General Information | Comment | Organism |
---|---|---|
additional information | the transportation of D-xylose across the cell membrane of Chlorella sorokiniana is realized by an inducible hexose symporter. The uptake of D-xylose subsequently activates the expression of key catalytic enzymes that enables D-xylose entering central metabolism | Chlorella sorokiniana |