Cloned (Comment) | Organism |
---|---|
gene lxr3, phylogenetic analysis, cloning in Escherichia coli strain JM109, expression of N-terminal His-tagged in Saccharomyces cerevisiae strain CEN.PK2-1D | Trichoderma reesei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of gene lxr3 leads to a significant reduction in NADPH specific LXR activity after replacement to both media containing L-arabinose | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NADPH | pH 7.0, 30°C, recombinant enzyme, with L-xylulose | Trichoderma reesei | |
16 | - |
L-xylulose | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
100 | - |
xylitol | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
105 | - |
D-ribulose | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
250 | - |
D-sorbitol | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000 | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NADP+ | Trichoderma reesei | - |
L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | Trichoderma reesei QM9414 | - |
L-xylulose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | Q8NK50 | gene lxr3 or tre60033 | - |
Trichoderma reesei QM9414 | Q8NK50 | gene lxr3 or tre60033 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminal His-tagged enzyme from Saccharomyces cerevisiae strain CEN.PK2-1D by nickel affinity chromatography | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH + H+ | low activity | Trichoderma reesei | ? + NADP+ | - |
? | |
D-ribulose + NADPH + H+ | - |
Trichoderma reesei | ? + NADP+ | - |
? | |
D-sorbitol + NADP+ | - |
Trichoderma reesei | D-sorbose + NADPH + H+ | - |
? | |
D-sorbitol + NADP+ | - |
Trichoderma reesei QM9414 | D-sorbose + NADPH + H+ | - |
? | |
D-xylulose + NADPH + H+ | low activity | Trichoderma reesei | xylitol + NADP+ | - |
? | |
L-sorbose + NADPH + H+ | low activity | Trichoderma reesei | L-sorbitol + NADP+ | - |
? | |
L-sorbose + NADPH + H+ | low activity | Trichoderma reesei QM9414 | L-sorbitol + NADP+ | - |
? | |
L-xylulose + NADPH + H+ | preferred substrate | Trichoderma reesei | xylitol + NADP+ | - |
r | |
L-xylulose + NADPH + H+ | preferred substrate | Trichoderma reesei QM9414 | xylitol + NADP+ | - |
r | |
additional information | substrate specificity, overview. No activity with L-xylo-3-hexulose, D-sorbose, D-ribitol, D-arabitol, or L-arabitol | Trichoderma reesei | ? | - |
? | |
additional information | substrate specificity, overview. No activity with L-xylo-3-hexulose, D-sorbose, D-ribitol, D-arabitol, or L-arabitol | Trichoderma reesei QM9414 | ? | - |
? | |
xylitol + NADP+ | - |
Trichoderma reesei | L-xylulose + NADPH + H+ | - |
r | |
xylitol + NADP+ | - |
Trichoderma reesei QM9414 | L-xylulose + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000 | Trichoderma reesei |
Synonyms | Comment | Organism |
---|---|---|
L-xylulose reductase | - |
Trichoderma reesei |
LXR3 | - |
Trichoderma reesei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
xylitol | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
1.8 | - |
D-sorbitol | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
7.75 | - |
NADPH | pH 7.0, 30°C, recombinant enzyme, with L-xylulose | Trichoderma reesei | |
8.24 | - |
D-ribulose | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei | |
11.4 | - |
L-xylulose | pH 7.0, 30°C, recombinant enzyme | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction reaction | Trichoderma reesei |
9 | - |
oxidation reaction | Trichoderma reesei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Trichoderma reesei | |
NADPH | - |
Trichoderma reesei |
Organism | Comment | Expression |
---|---|---|
Trichoderma reesei | transcription of lxr3 is specifically induced by L-arabinose and L-arabitol | up |
General Information | Comment | Organism |
---|---|---|
evolution | L-xylulose reductases belong to the superfamily of short chain dehydrogenases and reductases (SDRs) | Trichoderma reesei |
malfunction | deletion of lxr3 affects growth on L-arabinose and L-arabitol and reduces total NADPH-dependent LXR activity in cell free extracts | Trichoderma reesei |
metabolism | the enzyme catalyzes the NADPH-dependent reduction of L-xylulose to xylitol in L-arabinose and glucuronic acid catabolism | Trichoderma reesei |
physiological function | the L-xylulose reductase is essential for L-arabinose catabolism in Trichoderma reesei | Trichoderma reesei |