Cloned (Comment) | Organism |
---|---|
construction of a cDNA library, DNA sequence determination and analysis, subcloning in Escherichia coli DH5alpha, overexpression as His-tagged enzyme in Saccharomyces cerevisiae strain CEN.PK2 | Ambrosiozyma monospora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
D-arabinitol | pH 9.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
4.7 | - |
D-ribulose | pH 7.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
7.2 | - |
L-xylitol | pH 9.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora | |
9.6 | - |
L-xylulose | pH 7.0, 30°C, purified recombinant enzyme | Ambrosiozyma monospora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ambrosiozyma monospora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-xylulose + NADH | Ambrosiozyma monospora | part of the L-arabinose catabolism | L-xylitol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ambrosiozyma monospora | Q70FD1 | fragment; yeast, strain NRRL Y-1484 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Saccharomyces cerevisiae by nickel affinity chromatography | Ambrosiozyma monospora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose + NADH | - |
Ambrosiozyma monospora | D-arabinitol + NAD+ | - |
r | |
D-xylulose + NADH + H+ | about 100fold lower activity compared to L-xylulose | Ambrosiozyma monospora | D-arabinitol + NAD+ | - |
r | |
L-xylulose + NADH | - |
Ambrosiozyma monospora | L-xylitol + NAD+ | - |
r | |
L-xylulose + NADH | part of the L-arabinose catabolism | Ambrosiozyma monospora | L-xylitol + NAD+ | - |
r | |
additional information | NADH-linked enzyme form, no activity with NADPH and NADP+, no activity with L-arabinitol, adonitol or ribitol, dulcitol or galactitol, D-mannitol, and D-sorbitol, no activity with D-sorbose, L-sorbose, D-psicose, and D-fructose | Ambrosiozyma monospora | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ambrosiozyma monospora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
forward, reduction reaction | Ambrosiozyma monospora |
9 | - |
reverse, oxidation reaction | Ambrosiozyma monospora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for, no activity with NADP+ | Ambrosiozyma monospora | |
NADH | specific for no activity with NADPH | Ambrosiozyma monospora |