Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.1 extracted from

  • Takeda, M.; Anamizu, S.; Motomatsu, S.; Chen, X.; Thapa Chhetri, R.
    Identification and characterization of a mycobacterial NAD+-dependent alcohol dehydrogenase with superior reduction of diacetyl to (S)-acetoin (2014), Biosci. Biotechnol. Biochem., 78, 1879-1886.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NAD+ cosubstrate butan-1-ol, pH 7.0, 30°C Mycobacterium sp.
0.09
-
NADH cosubstrate butanal, pH 7.0, 30°C Mycobacterium sp.
0.48
-
Butanal cosubstrate NADH, pH 7.0, 30°C Mycobacterium sp.
5.65
-
butan-1-ol cosubstrate NAD+, pH 7.0, 30°C Mycobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
Mycobacterium sp.
150000
-
gel filtration Mycobacterium sp.

Organism

Organism UniProt Comment Textmining
Mycobacterium sp. W8VSK8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-pentanediol + NAD+ 18% of the activity with butan-1-ol Mycobacterium sp. ? + NADH + H+
-
?
butan-1-ol + NAD+ best substrate Mycobacterium sp. butanal + NADH + H+
-
r
butanal + NADH + H+
-
Mycobacterium sp. butan-1-ol + NAD+
-
r
ethanol + NAD+ 12% of the activity with butan-1-ol Mycobacterium sp. acetaldehyde + NADH + H+
-
r
additional information enzyme shows activity as a reductase specific for (S)-acetoin, EC 1.1.1.76, and both diacetyl reductase (EC 1.1.1.304) and NAD+-dependent alcohol dehydrogenase (EC 1.1.1.1) activities Mycobacterium sp. ?
-
?
propan-1-ol + NAD+ 55% of the activity with butan-1-ol Mycobacterium sp. propanal + NADH + H+
-
r
propan-2-ol + NAD+ 65% of the activity with butan-1-ol Mycobacterium sp. acetone + NADH + H+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 36000, SDS-PAGE, 4 * 35971, calculated Mycobacterium sp.

Synonyms

Synonyms Comment Organism
ADS1
-
Mycobacterium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
NAD+ cosubstrate butan-1-ol, pH 7.0, 30°C Mycobacterium sp.
2
-
butan-1-ol cosubstrate NAD+, pH 7.0, 30°C Mycobacterium sp.
102
-
Butanal cosubstrate NADH, pH 7.0, 30°C Mycobacterium sp.
153
-
NADH cosubstrate butanal, pH 7.0, 30°C Mycobacterium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium sp. isoelectric focusing
-
4.8
Mycobacterium sp. calculated
-
5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
butan-1-ol cosubstrate NAD+, pH 7.0, 30°C Mycobacterium sp.
80
-
NAD+ cosubstrate butan-1-ol, pH 7.0, 30°C Mycobacterium sp.
210
-
Butanal cosubstrate NADH, pH 7.0, 30°C Mycobacterium sp.
2000
-
NADH cosubstrate butanal, pH 7.0, 30°C Mycobacterium sp.