Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Saccharomyces cerevisiae |
expressed in Escherichia coli DH5alpha cells | Saccharomyces pastorianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | benzaldehyde | in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces pastorianus | |
2.9 | - |
p-Methoxybenzaldehyde | isozyme ADH1, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
3.4 | - |
p-Methoxybenzaldehyde | in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces pastorianus | |
5.9 | - |
p-Methoxybenzaldehyde | isozyme ADH2, in the presence of 0.1 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
6.9 | - |
p-methoxybenzyl alcohol | isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
11 | - |
p-methoxybenzyl alcohol | isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
14 | - |
benzaldehyde | isozyme ADH2, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
22 | - |
p-methoxybenzyl alcohol | in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces pastorianus | |
24 | - |
benzyl alcohol | in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces pastorianus | |
33 | - |
benzaldehyde | isozyme ADH1, in the presence of 0.25 mM NADH, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
34 | - |
benzyl alcohol | isozyme ADH1, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae | |
49 | - |
benzyl alcohol | isozyme ADH2, in the presence of 2 mM NAD+, in 83 mM potassium phosphate, 40 mM KCl, and 0.25 mM EDTA buffer, pH 7.3, at 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
cytoplasm | - |
Saccharomyces pastorianus | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces pastorianus | B6UQD0 | - |
- |
Saccharomyces pastorianus Y379-50 | B6UQD0 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose CL-4B column chromatograhy and octyl-Sepharose column chromatography | Saccharomyces cerevisiae |
DEAE-Sepharose CL-4B column chromatograhy and octyl-Sepharose column chromatography | Saccharomyces pastorianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NAD+ | - |
Saccharomyces cerevisiae | 2-butanone + NADH + H+ | - |
? | |
(R)-2-butanol + NAD+ | - |
Saccharomyces pastorianus | 2-butanone + NADH + H+ | - |
? | |
(R)-2-butanol + NAD+ | - |
Saccharomyces pastorianus Y379-50 | 2-butanone + NADH + H+ | - |
? | |
(R,S)-2-methylbutan-1-ol + NAD+ | - |
Saccharomyces cerevisiae | (R,S)-2-methyl-butan-1-one + NADH + H+ | - |
? | |
(R,S)-2-methylbutan-1-ol + NAD+ | - |
Saccharomyces pastorianus | (R,S)-2-methyl-butan-1-one + NADH + H+ | - |
? | |
(R,S)-2-methylbutan-1-ol + NAD+ | - |
Saccharomyces pastorianus Y379-50 | (R,S)-2-methyl-butan-1-one + NADH + H+ | - |
? | |
(S)-2-butanol + NAD+ | - |
Saccharomyces cerevisiae | 2-butanone + NADH + H+ | - |
? | |
(S)-2-butanol + NAD+ | - |
Saccharomyces pastorianus | 2-butanone + NADH + H+ | - |
? | |
(S)-2-butanol + NAD+ | - |
Saccharomyces pastorianus Y379-50 | 2-butanone + NADH + H+ | - |
? | |
(S)-2-methylbutan-1-ol + NAD+ | - |
Saccharomyces cerevisiae | (S)-2-methyl-butanal + NADH + H+ | - |
? | |
(S)-2-methylbutan-1-ol + NAD+ | - |
Saccharomyces pastorianus | (S)-2-methyl-butanal + NADH + H+ | - |
? | |
2-methylpropan-1-ol + NAD+ | - |
Saccharomyces cerevisiae | 2-methyl-propan-1-one + NADH + H+ | - |
? | |
2-methylpropan-1-ol + NAD+ | - |
Saccharomyces pastorianus | 2-methyl-propan-1-one + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Saccharomyces cerevisiae | acetone + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Saccharomyces pastorianus | acetone + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Saccharomyces pastorianus Y379-50 | acetone + NADH + H+ | - |
? | |
3-methylbutan-1-ol + NAD+ | - |
Saccharomyces cerevisiae | 3-methyl-butan-1-one + NADH + H+ | - |
? | |
3-methylbutan-1-ol + NAD+ | - |
Saccharomyces pastorianus | 3-methyl-butan-1-one + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | - |
Saccharomyces cerevisiae | benzaldehyde + NADH + H+ | - |
r | |
benzyl alcohol + NAD+ | - |
Saccharomyces pastorianus | benzaldehyde + NADH + H+ | - |
r | |
benzyl alcohol + NAD+ | - |
Saccharomyces pastorianus Y379-50 | benzaldehyde + NADH + H+ | - |
r | |
octanol + NAD+ | - |
Saccharomyces cerevisiae | octanal + NADH + H+ | - |
? | |
octanol + NAD+ | - |
Saccharomyces pastorianus | octanal + NADH + H+ | - |
? | |
p-methoxybenzyl alcohol + NAD+ | - |
Saccharomyces cerevisiae | p-methoxybenzaldehyde + NADH + H+ | - |
r | |
p-methoxybenzyl alcohol + NAD+ | - |
Saccharomyces pastorianus | p-methoxybenzaldehyde + NADH + H+ | - |
r | |
pentanol + NAD+ | - |
Saccharomyces cerevisiae | pentanal + NADH + H+ | - |
? | |
pentanol + NAD+ | - |
Saccharomyces pastorianus | pentanal + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH1 | - |
Saccharomyces pastorianus |
ADH1 | isozyme | Saccharomyces cerevisiae |
ADH2 | isozyme | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae | |
NAD+ | - |
Saccharomyces pastorianus |