Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109(DE3) cells | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
E97C | shows the same activity but a reduced thermostability with respect to the wild type recombinant protein | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl Butyl 650 S column chromatography, Q Sepharose fast flow column chromatography, Superose 12 prep-grade gel filtration, and TosoHaas G 2000 SWXL gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
wild type recombinant enzyme, from crude extract, at 65°C | Saccharolobus solfataricus |
0.02 | - |
mutant recombinant enzyme E97C, from crude extract, at 65°C | Saccharolobus solfataricus |
4.5 | - |
wild type recombinant enzyme, after 450fold purification, at 65°C | Saccharolobus solfataricus |
5.2 | - |
mutant recombinant enzyme E97C, after 260fold purification, at 65°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | - |
Saccharolobus solfataricus | benzaldehyde + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SSADH | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the activity of the recombinant wild type enzyme slowly decreases to 50% after 16 h at 65°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharolobus solfataricus |