Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 1 mM, 95% inhibition | Brevibacterium sp. | |
CuCl2 | 1 mM, complete inhibition | Brevibacterium sp. | |
HgCl2 | 1 mM, complete inhibition | Brevibacterium sp. | |
NiCl2 | 1 mM, 93% inhibition | Brevibacterium sp. | |
PbCl2 | 1 mM, 24% inhibition | Brevibacterium sp. | |
PCMB | 1 mM, complete inhibition | Brevibacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
benzyl alcohol | - |
Brevibacterium sp. | |
0.02 | - |
(R)-2-phenylpropanol | - |
Brevibacterium sp. | |
0.025 | - |
1-phenylethanol | - |
Brevibacterium sp. | |
0.033 | - |
3-phenylpropanol | - |
Brevibacterium sp. | |
0.157 | - |
(S)-2-phenylpropanol | - |
Brevibacterium sp. | |
0.26 | - |
phenylacetaldehyde | - |
Brevibacterium sp. | |
0.864 | - |
2-phenylpropionaldehyde | - |
Brevibacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Brevibacterium sp. |
39000 | - |
1 * 39000, SDS-PAGE | Brevibacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacterium sp. | - |
KU 1309 | - |
Brevibacterium sp. KU 1309 | - |
KU 1309 | - |
Purification (Comment) | Organism |
---|---|
- |
Brevibacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-phenylpropanol + NAD+ | 63% of the activity with 2-phenylethanol | Brevibacterium sp. | (R)-2-phenylpropanal + NADH + H+ | - |
? | |
(R)-2-phenylpropanol + NAD+ | 63% of the activity with 2-phenylethanol | Brevibacterium sp. KU 1309 | (R)-2-phenylpropanal + NADH + H+ | - |
? | |
(S)-2-phenylpropanol + NAD+ | 156% of the activity with 2-phenylethanol | Brevibacterium sp. | (S)-2-phenylpropanal + NADH + H+ | - |
? | |
1-butanol + NAD+ | 111% of the activity with 2-phenylethanol | Brevibacterium sp. | butanal + NADH | - |
? | |
1-butanol + NAD+ | 111% of the activity with 2-phenylethanol | Brevibacterium sp. KU 1309 | butanal + NADH | - |
? | |
1-dodecanol + NAD+ | 68% of the activity with 2-phenylethanol | Brevibacterium sp. | dodecanal + NADH | - |
? | |
1-dodecanol + NAD+ | 68% of the activity with 2-phenylethanol | Brevibacterium sp. KU 1309 | dodecanal + NADH | - |
? | |
1-octanol + NAD+ | 101% of the activity with 2-phenylethanol | Brevibacterium sp. | octanal + NADH | - |
? | |
1-octanol + NAD+ | 101% of the activity with 2-phenylethanol | Brevibacterium sp. KU 1309 | octanal + NADH | - |
? | |
1-phenylethanol + NAD+ | 46% of the activity with 2-phenylethanol | Brevibacterium sp. | 1-phenylethanone + NADH | - |
? | |
2-phenylethanol + NAD+ | - |
Brevibacterium sp. | phenylacetaldehyde + NADH + H+ | - |
r | |
2-phenylpropionaldehyde + NADH + H+ | - |
Brevibacterium sp. | 2-phenylpropanol + NAD+ | - |
r | |
2-propanol + NAD+ | 54% of the activity with 2-phenylethanol | Brevibacterium sp. | 2-propanone + NADH | - |
? | |
3-phenylpropanol + NAD+ | 135% of the activity with 2-phenylethanol | Brevibacterium sp. | 3-phenylpropanal + NADH + H+ | - |
? | |
benzyl alcohol + NAD+ | 199% of the activity with 2-phenylethanol | Brevibacterium sp. | benzaldehyde + NADH + H+ | - |
? | |
ethanol + NAD+ | 76% of the activity with 2-phenylethanol | Brevibacterium sp. | acetaldehyde + NADH | - |
? | |
ethanol + NAD+ | 76% of the activity with 2-phenylethanol | Brevibacterium sp. KU 1309 | acetaldehyde + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Brevibacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Brevibacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, no significant loss of activity | Brevibacterium sp. |
50 | - |
30 min, 50% loss of activity | Brevibacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of phenylacetaldehyde | Brevibacterium sp. |
10.4 | - |
oxidation of 2-phenylethanol | Brevibacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5.0: about 50% of maximal activity, pH 8.0: about 40% of maximal activity, reduction of phenylacetaldehyde | Brevibacterium sp. |
8 | 12 | pH 8.0: about 50% of maximal activity, pH 12.0: about 50% of maximal activity, oxidation of 2-phenylethanol | Brevibacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADP+ shows less than 0.1% of the activity with NAD+ | Brevibacterium sp. | |
NAD+ | - |
Brevibacterium sp. | |
NADH | - |
Brevibacterium sp. |