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Literature summary for 1.1.1.1 extracted from

  • Hirano, J.; Miyamoto, K.; Ohta, H.
    Purification and characterization of the alcohol dehydrogenase with a broad substrate specificity originated from 2-phenylethanol-assimilating Brevibacterium sp. KU 1309 (2005), J. Biosci. Bioeng., 100, 318-322.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 1 mM, 95% inhibition Brevibacterium sp.
CuCl2 1 mM, complete inhibition Brevibacterium sp.
HgCl2 1 mM, complete inhibition Brevibacterium sp.
NiCl2 1 mM, 93% inhibition Brevibacterium sp.
PbCl2 1 mM, 24% inhibition Brevibacterium sp.
PCMB 1 mM, complete inhibition Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
benzyl alcohol
-
Brevibacterium sp.
0.02
-
(R)-2-phenylpropanol
-
Brevibacterium sp.
0.025
-
1-phenylethanol
-
Brevibacterium sp.
0.033
-
3-phenylpropanol
-
Brevibacterium sp.
0.157
-
(S)-2-phenylpropanol
-
Brevibacterium sp.
0.26
-
phenylacetaldehyde
-
Brevibacterium sp.
0.864
-
2-phenylpropionaldehyde
-
Brevibacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Brevibacterium sp.
39000
-
1 * 39000, SDS-PAGE Brevibacterium sp.

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
KU 1309
-
Brevibacterium sp. KU 1309
-
KU 1309
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-phenylpropanol + NAD+ 63% of the activity with 2-phenylethanol Brevibacterium sp. (R)-2-phenylpropanal + NADH + H+
-
?
(R)-2-phenylpropanol + NAD+ 63% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 (R)-2-phenylpropanal + NADH + H+
-
?
(S)-2-phenylpropanol + NAD+ 156% of the activity with 2-phenylethanol Brevibacterium sp. (S)-2-phenylpropanal + NADH + H+
-
?
1-butanol + NAD+ 111% of the activity with 2-phenylethanol Brevibacterium sp. butanal + NADH
-
?
1-butanol + NAD+ 111% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 butanal + NADH
-
?
1-dodecanol + NAD+ 68% of the activity with 2-phenylethanol Brevibacterium sp. dodecanal + NADH
-
?
1-dodecanol + NAD+ 68% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 dodecanal + NADH
-
?
1-octanol + NAD+ 101% of the activity with 2-phenylethanol Brevibacterium sp. octanal + NADH
-
?
1-octanol + NAD+ 101% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 octanal + NADH
-
?
1-phenylethanol + NAD+ 46% of the activity with 2-phenylethanol Brevibacterium sp. 1-phenylethanone + NADH
-
?
2-phenylethanol + NAD+
-
Brevibacterium sp. phenylacetaldehyde + NADH + H+
-
r
2-phenylpropionaldehyde + NADH + H+
-
Brevibacterium sp. 2-phenylpropanol + NAD+
-
r
2-propanol + NAD+ 54% of the activity with 2-phenylethanol Brevibacterium sp. 2-propanone + NADH
-
?
3-phenylpropanol + NAD+ 135% of the activity with 2-phenylethanol Brevibacterium sp. 3-phenylpropanal + NADH + H+
-
?
benzyl alcohol + NAD+ 199% of the activity with 2-phenylethanol Brevibacterium sp. benzaldehyde + NADH + H+
-
?
ethanol + NAD+ 76% of the activity with 2-phenylethanol Brevibacterium sp. acetaldehyde + NADH
-
?
ethanol + NAD+ 76% of the activity with 2-phenylethanol Brevibacterium sp. KU 1309 acetaldehyde + NADH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 39000, SDS-PAGE Brevibacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
-
Brevibacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
30 min, no significant loss of activity Brevibacterium sp.
50
-
30 min, 50% loss of activity Brevibacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of phenylacetaldehyde Brevibacterium sp.
10.4
-
oxidation of 2-phenylethanol Brevibacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 40% of maximal activity, reduction of phenylacetaldehyde Brevibacterium sp.
8 12 pH 8.0: about 50% of maximal activity, pH 12.0: about 50% of maximal activity, oxidation of 2-phenylethanol Brevibacterium sp.

Cofactor

Cofactor Comment Organism Structure
additional information NADP+ shows less than 0.1% of the activity with NAD+ Brevibacterium sp.
NAD+
-
Brevibacterium sp.
NADH
-
Brevibacterium sp.