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Literature summary for 1.1.1.1 extracted from
- Purification and characterization of two NAD-dependent alcohol dehydrogenases (ADHs) induced in the quinoprotein ADH-deficient mutant of Acetobacter pasteurianus SKU1108 (2003), Biosci. Biotechnol. Biochem., 67, 958-965.
General Stability
| General Stability | Organism |
|---|---|
| isozymes are stabilized by MgCl2 and DTT during purification | Acetobacter pasteurianus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 67% inhibition of ADH II at 5 mM, 45% inhibition of ADH I at 1 mM, irreversible inhibition, addition of Mg2+ and Zn2+ increase the inhibitory effect | Acetobacter pasteurianus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
NADH | isozyme ADH II, pH 6.0, 25°C | Acetobacter pasteurianus | |
| 0.04 | - |
NAD+ | isozyme ADH II, pH 8.5, 25°C | Acetobacter pasteurianus | |
| 0.2 | - |
NADH | isozyme ADH I, pH 5.5, 25°C | Acetobacter pasteurianus | |
| 0.3 | - |
acetaldehyde | isozyme ADH I, pH 5.5, 25°C | Acetobacter pasteurianus | |
| 1.2 | - |
NAD+ | isozyme ADH I, pH 8.5, 25°C | Acetobacter pasteurianus | |
| 2.3 | - |
ethanol | isozyme ADH I, pH 8.5, 25°C | Acetobacter pasteurianus | |
| 7.3 | - |
ethanol | isozyme ADH II, pH 8.5, 25°C | Acetobacter pasteurianus | |
| 24 | - |
acetaldehyde | isozyme ADH II, pH 6.0, 25°C | Acetobacter pasteurianus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | 2 isozymes | Acetobacter pasteurianus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | ADH I contains 1 Zn2+ per subunit, while ADH II does not contains any metal ions | Acetobacter pasteurianus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
2 * 31000, ADH II, SDS-PAGE | Acetobacter pasteurianus |
| 42000 | - |
3 * 42000, ADH I, SDS-PAGE | Acetobacter pasteurianus |
| 70000 | - |
ADH II, grl filtration | Acetobacter pasteurianus |
| 130000 | - |
ADH I, gel filtration | Acetobacter pasteurianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter pasteurianus | - |
2 soluble isozymes | - |
| Acetobacter pasteurianus SKU1108 | - |
2 soluble isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes from cytosol by DEAE ion exchange, hydroxyl apatite, and gel filtration chromatography, ADH I 35fold, ADH II 21fold | Acetobacter pasteurianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of the 2 isozmyes with various substrates, overview | Acetobacter pasteurianus |
| 361 | - |
purified ADH II | Acetobacter pasteurianus |
| 597 | - |
purified ADH I | Acetobacter pasteurianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| butyraldehyde + NADH + H+ | best substrate for isozyme ADH II | Acetobacter pasteurianus | n-butanol + NAD+ | - |
r | |
| butyraldehyde + NADH + H+ | best substrate for isozyme ADH II | Acetobacter pasteurianus SKU1108 | n-butanol + NAD+ | - |
r | |
| ethanol + NAD+ | - |
Acetobacter pasteurianus | acetaldehyde + NADH | acetaldehyde is the best substrate for isozyme ADH I | r | |
| ethanol + NAD+ | - |
Acetobacter pasteurianus SKU1108 | acetaldehyde + NADH | acetaldehyde is the best substrate for isozyme ADH I | r | |
| additional information | substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers | Acetobacter pasteurianus | ? | - |
? | |
| additional information | substrate specificity of the 2 isozmyes with various substrates, overview, isozymes are highly specific for the (R)-stereoisomers and enantioselctive for the R(-)isomers | Acetobacter pasteurianus SKU1108 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 31000, ADH II, SDS-PAGE | Acetobacter pasteurianus |
| trimer | 3 * 42000, ADH I, SDS-PAGE | Acetobacter pasteurianus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH | - |
Acetobacter pasteurianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at, forward and reverse reaction | Acetobacter pasteurianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at, reverse reaction, ADH I | Acetobacter pasteurianus |
| 6 | - |
assay at, reverse reaction, ADH II | Acetobacter pasteurianus |
| 8.5 | - |
assay at, forward reaction, ADH I and ADH II | Acetobacter pasteurianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | dependent on | Acetobacter pasteurianus | |
| NADH | dependent on | Acetobacter pasteurianus | |
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