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Literature summary for 1.1.1.1 extracted from

  • Weinhold, E.G.; Benner, S.A.
    Engineering yeast alcohol dehydrogenase. Replacing Trp54 by Leu broadens substrate specificity (1995), Protein Eng., 8, 457-461.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
W54L less active than the wild-type enzyme with ethanol, 1-propanol and 1-butanol. With 1-pentanol and 1-hexanol the mutant enzyme is a better catalyst than the wild-type enzyme Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
cinnamyl alcohol mutant enzyme W54L Equus caballus
4
-
ethanol wild-type enzyme Adh1 Equus caballus
4.58
-
cinnamyl alcohol wilde-type enzyme ADh1 Equus caballus
6.5
-
Hexanol mutant enzyme W54L Equus caballus
6.7
-
4-Methyl-1-pentanol mutant enzyme W54L Equus caballus
11.8
-
Pentanol mutant enzyme W54L Equus caballus
13.4
-
ethanol mutant enzyme W54L Equus caballus
13.6
-
Propanol wilde-type enzyme ADh1 Equus caballus
13.8
-
Hexanol wilde-type enzyme ADh1 Equus caballus
29.4
-
butanol mutant enzyme W54L Equus caballus
43.9
-
Propanol mutant enzyme W54L Equus caballus
53.8
-
4-Methyl-1-pentanol wilde-type enzyme ADh1 Equus caballus
53.9
-
butanol wilde-type enzyme ADh1 Equus caballus
56.2
-
Pentanol wilde-type enzyme ADh1 Equus caballus
93.3
-
butan-2-ol wilde-type enzyme ADh1 Equus caballus
193
-
propan-2-ol mutant enzyme W54L Equus caballus
268
-
2-propanol wilde-type enzyme ADh1 Equus caballus

Organism

Organism UniProt Comment Textmining
Equus caballus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Equus caballus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Equus caballus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methyl-1-butanol + NAD+
-
Equus caballus ? + NADH
-
?
4-methyl-1-pentanol + NAD+
-
Equus caballus 4-methyl-1-pentanal + NADH
-
?
butan-2-ol + NAD+
-
Equus caballus butan-2-one + NADH
-
?
cinnamyl alcohol + NAD+
-
Equus caballus cinnamaldehyde + NADH
-
?
ethanol + NAD+
-
Equus caballus acetaldehyde + NADH
-
?
hexanol + NAD+
-
Equus caballus n-hexanal + NADH
-
?
pentanal + NAD+
-
Equus caballus pentanone + NADH
-
?
propan-2-ol + NAD+
-
Equus caballus acetone + NADH
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
3-methyl-1-butanol mutant enzyme W54L Equus caballus
0.27
-
3-methyl-1-butanol wild-type enzyme Adh 1 Equus caballus
0.31
-
butan-2-ol mutant enzyme W54L Equus caballus
5.8
-
propan-2-ol mutant enzyme W54L Equus caballus
6.7
-
4-Methyl-1-pentanol wild-type enzyme Adh 1 Equus caballus
11.9
-
butan-2-ol wild-type enzyme Adh 1 Equus caballus
18.4
-
Pentanol mutant enzyme W54L Equus caballus
18.6
-
4-Methyl-1-pentanol mutant enzyme W54L Equus caballus
23.9
-
Hexanol mutant enzyme W54L Equus caballus
28.7
-
Propanol mutant enzyme W54L Equus caballus
29.6
-
Hexanol wild-type enzyme Adh 1 Equus caballus
32.7
-
butanol mutant enzyme W54L Equus caballus
53.1
-
propan-2-ol wild-type enzyme Adh 1 Equus caballus
56.2
-
Pentanol wild-type enzyme Adh 1 Equus caballus
98
-
cinnamyl alcohol mutant enzyme W54L Equus caballus
102
-
ethanol mutant enzyme W54L Equus caballus
118
-
butanol wild-type enzyme Adh 1 Equus caballus
123
-
ethanol wild-type enzyme Adh 1 Equus caballus
133
-
cinnamyl alcohol wild-type enzyme Adh 1 Equus caballus
308
-
ethanol wild-type enzyme Adh 1 Equus caballus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Equus caballus
NADH
-
Equus caballus