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Literature summary for 1.1.1.1 extracted from

  • Rudge, J.; Bickerstaff, G.F.
    Purification and properties of an alcohol dehydrogenase from Sporotrichum pulverulentum (1986), Enzyme Microb. Technol., 8, 120-124.
No PubMed abstract available
Search for more literature for 1.1.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
 acetaldehyde
-
 Sporotrichum pulverulentum
0.021
-
 NADH
-
 Sporotrichum pulverulentum
0.17
-
 NAD+
-
 Sporotrichum pulverulentum
2.5
-
 ethanol
-
 Sporotrichum pulverulentum
64
-
 ethanol
-
 Sporotrichum pulverulentum
64
-
 propan-2-ol
-
 Sporotrichum pulverulentum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, SDS-PAGE Sporotrichum pulverulentum
176000
-
 non-denaturing PAGE Sporotrichum pulverulentum

Organism

Organism UniProt Comment Textmining
Sporotrichum pulverulentum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sporotrichum pulverulentum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
196.3
-
-
 Sporotrichum pulverulentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ethylhexan-1-ol + NAD+ weak activity Sporotrichum pulverulentum 2-ethylhexanal + NADH
-
 ?
2-methylpropan-1-ol + NAD+
-
 Sporotrichum pulverulentum 2-methyl-propan-1-one + NADH
-
 ?
acetaldehyde + NADH + H+
-
 Sporotrichum pulverulentum ethanol + NAD+
-
 r
butan-2-ol + NAD+
-
 Sporotrichum pulverulentum butan-2-one + NADH
-
 ?
ethanol + NAD+
-
 Sporotrichum pulverulentum acetaldehyde + NADH
-
 r
hexanol + NAD+
-
 Sporotrichum pulverulentum n-hexanal + NADH
-
 ?
methanol + NAD+ no activity Sporotrichum pulverulentum formaldehyde + NADH + H+
-
 ?
octan-1-ol + NAD+ weak activity Sporotrichum pulverulentum n-octanal + NADH
-
 ?
octan-2-ol + NAD+ weak activity Sporotrichum pulverulentum octan-2-one + NADH
-
 ?
pentan-2-ol + NAD+
-
 Sporotrichum pulverulentum 2-pentanone + NADH
-
 ?
pentanal + NAD+
-
 Sporotrichum pulverulentum pentanone + NADH
-
 ?
propan-2-ol + NAD+
-
 Sporotrichum pulverulentum acetone + NADH
-
 ?
propanol + NAD+
-
 Sporotrichum pulverulentum propionaldehyde + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, SDS-PAGE Sporotrichum pulverulentum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Sporotrichum pulverulentum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 10°C: about 65% of maximal activity, 50°C: about 40% of maximal activity Sporotrichum pulverulentum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6 6.5 oxidation of ethanol Sporotrichum pulverulentum
8 8.4 reduction of aldehyde Sporotrichum pulverulentum

pH Range

pH Minimum pH Maximum Comment Organism
5.2 6.8 pH 5.2: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, oxidation of ethanol Sporotrichum pulverulentum
7.2 9.2 pH 7.2: about 50% of maximal activity, pH 9.2: about 60% of maximal activity Sporotrichum pulverulentum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sporotrichum pulverulentum
NADH
-
 Sporotrichum pulverulentum