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NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
ATP + 5'-phosphate terminus of deoxyribonucleotides
?
-
-
-
-
?
ATP + nicked DNA
?
-
-
-
-
?
ATP + nicked DNA
AMP + diphosphate + ?
-
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)n+m
-
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
(deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide
-
-
-
-
?
NADH + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
-
NADH has a significantly higher Km as NAD+
-
-
?
Thionicotinamide derivative of NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
-
significantly higher Km as NAD+
-
-
?
additional information
?
-
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
-
DNA ligase plays the key role in DNA replication of joining the nascent small pieces of DNA at the replication fork. The enzyme participates in the synthesis and repair of DNA
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
-
the enzyme is indispensable for normal cell growth and inviability of mutants seems to be primarily the result of an inability to seal Okazaki fragments
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
?
-
joining of short DNA fragments formed during DNA replication and so enabling DNA synthesis to progress in an overall 3'-5' direction on the antiparallel strand of the double helix, while continual 5'-3' synthesis proceeds on the other strand. Plays a role during genetic recombination and in the repair of UV-damaged DNA
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
-
-
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
joining of the 5'-phosphoryl of a DNA chain to the 3'-hydroxyl of an RNA molecule in two ways: 1. Oligo(A) is joined to the 5'-phosphoryl of oligo(dA) on a poly(dT) template strand. 2. the enzyme can circularize poly[d(A-T)]pU in which UMP occupies the 3'-hydroxyl terminal position
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
blunt-end ligation in presence of macromolecular solutes, inactive in absence
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
oligonucleotides as short as six or seven in length can be joined if annealed to long complementary deoxyribonucleotides
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
joining of 5'-phosphoryl terminus of DNA chain to the 3'-hydroxyl terminus of RNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA ligase requires two DNA termini, the 5'-terminus, carrying a phosphate group, and the 3'-terminus, a hydroxyl group. These termini must reside on a double-stranded molecule, DNA:DNA or DNA:RNA. Both strands of the duplex may terminate, in the form of a staggered end or a blunt end, and the ligase then requires a second similar double-stranded terminus to join the two in an intermolecular reaction. Alternatively, the two termini may be provided by a nick in just one strand of a duplex, which the enzyme will then seal
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
formation of linear oligomers by intermolecular cohesive-end ligation
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
the self-complementary polymer, poly(dA-dT), forms a looped-back structure that DNA ligase can join to yield a circular molecule
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
joins oligo(dT) that is base-paired to poly(dA) or oligo(dA) base-paired to poly(dT)
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
PvuII endonuclease-digested pBR322 DNA, formation of linear oligomers ranging from dimer through pentamer
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
formation of linear oligomers by intermolecular blunt-end ligation in presence of high concentrations of polymer polyethylene glycol
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
catalyzes the joining of polynucleotide strands provided they have juxtaposed 3'-hydroxyl and 5'-phosphoryl end groups aligned in a duplex structure: e.g. annealed ends of lamdda DNA, endogenous nicks in T5 DNA, interruptions created by the action of pancreatic DNAse, annealed fragments generated by the staggered cutting action of some restriction endonucleases
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
no ligation of blunt-ended or flush-ended DNAs
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA
-
?
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
in addition to the unique N-terminal domain Ia that stimulates ligase-AMP formation, there are three C-terminal domains that extend from the OB domain: a small zinc-binding (Zn) domain, a helix-hairpin-helix domain, and a BRCA1 C-terminal domain
-
-
?
additional information
?
-
-
NAD+/nicotinamide nucleotide exchange reaction
-
-
?
additional information
?
-
-
no activity with NADH, NADP, NADPH, ATP and AMP
-
-
?
additional information
?
-
-
the enzyme is relatively tolerant of 5'-phosphate base mispairs and 5' N:abasic lesions
-
-
?
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(2R,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl dihydrogen phosphate
-
-
(3aR,5R,6R,6aS)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-6-hydroxy-2-sulfanyltetrahydro-2H-2lambda-5-furo[2,3-d][1,3,2]dioxaphosphol-2-one
-
-
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxamide
-
-
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxylic acid
-
-
(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)acetonitrile
-
-
(4R)-N4-(6-chloro-2-methoxyacridin-9-yl)-N1,N1-diethylpentane-1,4-diamine
-
-
2,2'-([4-[(6-chloro-2-methoxyacridin-9-yl)amino]pentyl]imino)diethanol
-
-
2,4-diamino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrile
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2,4-diamino-7-tert-butyl-4a,8a-dihydro-1,8-naphthyridine-3-carbonitrile
-
-
2-(butylsulfanyl)-5'-deoxyadenosine
-
-
2-(butylsulfanyl)adenosine
-
-
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
-
-
2-(cyclopentyloxy)-3',5'-dideoxy-3'-[(3,5-dichlorophenyl)methyl]adenosine
-
-
2-(cyclopentyloxy)-3',5'-dideoxy-5'-fluoro-3'-(1H-triaziren-1-yl)adenosine
-
-
2-(cyclopentyloxy)-5'-deoxy-5'-fluoroadenosine
-
-
2-(cyclopentyloxy)-5'-deoxyadenosine
-
-
2-(cyclopentyloxy)adenosine
-
-
2-(cyclopentylsulfanyl)adenosine
-
-
2-amino-5-(2-hydroxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-(2-methoxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-(aminomethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2-amino-5-oxo-5H-[1]benzopyrano[2,3-b]pyridine-3-carboxamide
-
-
2-amino-5-phenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5-[(3R)-3-aminopyrrolidin-1-yl]-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
2-amino-6-(2-phenylethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-(hydroxymethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-(propan-2-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-benzyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-benzyl-7-chloro-5-methyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-(3-hydroxyazetidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-(4-hydroxypiperidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-[(2-hydroxyethyl)(methyl)amino]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-5-[(3R)-3-hydroxypyrrolidin-1-yl]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-cyclopentyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-bromo-7-tert-butyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-chloro-5-ethyl-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-cyclopentyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-cyclopropyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-ethenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-ethyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-6-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7,8-difluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations, worse inhibitor than 2-amino-7-fluoro-5-oxo-4a,10a-dihydro-5H-chromeno[2,3-b]pyridine-3-carboxamide
2-amino-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-chloro-5-methyl-6-(prop-2-en-1-yl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-chloro-5-methyl-6-(pyridin-4-ylmethyl)-1,8-naphthyridine-3-carboxamide
-
-
2-amino-7-fluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations, better inhibitor than 2-amino-7,8-difluoro-5-oxo-4a,10a-dihydro-5H-chromeno[2,3-b]pyridine-3-carboxamide
2-amino-7-[2-(benzyloxy)propan-2-yl]-6-bromo-1,8-naphthyridine-3-carboxamide
-
-
2-[(bicyclo[3.1.0]hexan-3-yl)oxy]-5'-deoxyadenosine
-
-
2-[(decahydronaphthalen-2-yl)oxy]-5'-deoxyadenosine
-
-
2-[(decahydronaphthalen-2-yl)oxy]adenosine
-
-
3'-chloro-2-(cyclopentyloxy)-3',5'-dideoxyadenosine
-
-
3'-chloro-2-(cyclopentyloxy)-3'-deoxyadenosine
-
-
3'-chloro-3',5'-dideoxy-2-[(spiro[2.2]pentan-1-yl)methoxy]adenosine
-
-
3-(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)propanenitrile
-
-
3-amino-6-[(6-chloro-2-methoxyacridin-9-yl)amino]heptan-1-ol
-
-
3-chloro-9-[[5-(diethylammonio)pentan-2-yl]amino]-7-methoxyacridin-2-aminium
-
-
3-chloro-N9-[5-(diethylamino)pentan-2-yl]-7-methoxyacridine-2,9-diamine
-
-
3-[[[[(2R,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]oxy](hydroxy)phosphoryl]carbamoyl]-1-[5-O-(hydroxyphosphinato)-beta-D-ribofuranosyl]pyridin-1-ium
-
-
3-[[[[(2R,3S,4R,5R)-5-(6-amino-2-phenyl-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]oxy](hydroxy)phosphoryl]carbamoyl]-1-[5-O-(hydroxyphosphinato)-beta-D-ribofuranosyl]pyridin-1-ium
-
-
4-([4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)benzoic acid
-
-
4-amino-2-(1,4-dioxaspiro[4.5]dec-8-yloxy)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(2-fluoroethoxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylamino)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentyloxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylsulfanyl)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-(cyclopentylsulfanyl)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(1,3-difluoropropan-2-yl)oxy]-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2,4-dimethylcyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2-methylcyclohexyl)oxy]-8-(1H-pyrazol-4-ylmethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(2-methylcyclohexyl)oxy]-8-[(1R,5R)-7-oxo-6-oxabicyclo[3.2.1]oct-4-yl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-2-[(4-chlorocyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-6-(1H-benzimidazol-2-yl)pyridazin-3(2H)-one
-
-
4-amino-8-(2,3-dihydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(2-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(4-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(2-hydroxyethyl)-2-(propan-2-yloxy)pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(3-fluoro-2-hydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-(cyclopent-3-en-1-ylmethyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1R)-2-hydroxycyclopentyl]-2-[[4-(trifluoromethyl)cyclohexyl]oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1S)-2,3-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(1S,2S)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2R)-2-hydroxycyclohexyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2R)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(2S,5S)-2,5-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[(3,4-dihydroxycyclopentyl)methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[[(2R)-2-hydroxycyclopentyl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
4-amino-8-[[(4S)-4-hydroxytetrahydrofuran-3-yl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
-
-
5'-deoxy-2-(spiro[2.2]pent-1-ylmethoxy)adenosine
-
-
5'-deoxy-2-phenoxyadenosine
-
-
5'-deoxy-2-[(spiro[2.2]pentan-1-yl)methoxy]adenosine
-
-
5'-deoxy-2-[(trans-4-methylcyclohexyl)oxy]adenosine
-
-
5'-deoxy-2-[(tricyclo[3.3.1.1~3,7~]decan-2-yl)methoxy]adenosine
-
-
5'-deoxy-5'-fluoro-2-[(1,3-thiazol-2-yl)methoxy]adenosine
-
-
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
-
-
5'-deoxy-5'-fluoro-2-[[1-(hydroxymethyl)cyclopropyl]methoxy]adenosine
-
-
5,7-diamino-2-(2-methylpropyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-(cyclohexylmethyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-(propan-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-benzyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-cyclobutyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-cyclohexyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-cyclopentyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-cyclopropyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-methyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-phenyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(1,3-thiazol-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(1H-imidazol-1-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(3H-1,2,4-triazol-3-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(3H-pyrazol-3-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(4,5-dihydro-1,3-oxazol-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(4-cyanobutyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(4-methyl-1H-imidazol-1-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(4H-1,2,3-triazol-4-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(5-cyanopentyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(5-cyclohexylpentyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(methylsulfanyl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(pyridin-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(pyridin-3-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(pyridin-4-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(pyrimidin-4-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(thiophen-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-(thiophen-3-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-cyclopentyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-methyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-phenyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-propyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-[4-(hydroxymethyl)-1,3-thiazol-2-yl]-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4-[4-(methoxymethyl)-1,3-thiazol-2-yl]-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-2-tert-butyl-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5,7-diamino-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carbonitrile
-
-
5-(5,7-diamino-2-tert-butyl-6-cyano-4a,8a-dihydropyrido[2,3-d]pyrimidin-4-yl)thiophene-2-carboxylic acid
-
-
5-([4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)thiophene-2-carboxylic acid
-
-
6-chloro-2-methoxy-N-[4-(piperidin-1-yl)pentyl]acridin-9-amine
-
-
6-chloro-9-[[5-(diethylammonio)pentan-2-yl]amino]-2-methoxyacridinium
-
-
7-amino-2-tert-butyl-4-(1H-pyrrol-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carboxamide
-
-
7-methoxy-6-methylpteridine-2,4-diamine
-
-
ADP
-
inhibits adenylation reaction
AMP
-
inhibits adenylation reaction
ATP
-
inhibits adenylation reaction
cAMP
-
inhibits adenylation reaction
doxorubicin
-
doxorubicin inhibits the ATP-dependent DNA ligase of bacteriophage T4 and the Escherichia coli NAD+-dependent ligase with a similar potency
ethyl 2-(5,7-diamino-2-tert-butyl-6-cyano-4a,8a-dihydropyrido[2,3-d]pyrimidin-4-yl)-1,3-thiazole-4-carboxylate
-
-
ethyl 2-(5,7-diamino-2-tert-butyl-6-cyano-4a,8a-dihydropyrido[2,3-d]pyrimidin-4-yl)-1,3-thiazole-5-carboxylate
-
-
hydroxychloroquine
-
specific inhibitor of the Escherichia coli enzyme
Mn2+
-
activation at 0.2-1.0 mM, inhibition at higher concentration
N'-(6-chloro-2-methoxyacridin-9-yl)-N,N-diethylbutane-1,4-diamine
-
-
N'-(6-chloro-2-methoxyacridin-9-yl)-N,N-dimethylbutane-1,4-diamine
-
-
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-6-yl]sebacamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-8-yl]adipamide
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
N,N-bis(4-aminobutyl)-N'-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N,N-bis(4-aminobutyl)-N'-(6-chloro-2-methoxyacridin-9-yl)pentane-1,5-diamine
-
-
N-(4-aminobutyl)-N-(3-aminopropyl)-N'-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N-(4-aminobutyl)-N-(3-aminopropyl)-N'-(6-chloro-2-methoxyacridin-9-yl)pentane-1,5-diamine
-
-
N-(4-aminobutyl)-N-[3-[(6-chloro-2-methoxyacridin-9-yl)amino]propyl]butane-1,4-diamine
-
-
N-(6-chloro-2-methoxyacridin-9-yl)butane-1,4-diamine
-
-
N1,N1-bis(2-chloroethyl)-N4-(6-chloro-2-methoxyacridin-9-yl)pentane-1,4-diamine
-
-
NAD+
-
inhibitory above 0.1 mM, activity is abolished at 0.5 mM
pyridochromanones
-
differently substituted compounds tested and all found to be inhibitory, competitive with NAD+, inhibitory in nano-molar rage without affecting human DNA-ligase (ATP-depentend) activity
-
tert-butyl 3-(7-amino-3-bromo-6-carbamoyl-1,8-naphthyridin-2-yl)azetidine-1-carboxylate
-
-
additional information
-
not inhibited by cinchonidine, quinine, and N,N'-bis[4chloroquinolin-8-yl]succinamide
-
Chloroquine
-
-
Chloroquine
-
effective inhibitor
Quinacrine
-
-
Quinacrine
-
powerful inhibitor, exhibits good inhibition at nanomolar concentrations
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0.12
(2R,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl dihydrogen phosphate
Escherichia coli
-
pH and temperature not specified in the publication
0.0015
(3aR,5R,6R,6aS)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-6-hydroxy-2-sulfanyltetrahydro-2H-2lambda-5-furo[2,3-d][1,3,2]dioxaphosphol-2-one
Escherichia coli
-
pH and temperature not specified in the publication
0.0000488
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.000051
(3S)-3-[4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]-4-hydroxycyclopentanecarboxylic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.00002
(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)acetonitrile
Escherichia coli
-
pH and temperature not specified in the publication
0.12
(Rp)-3',5'-cyclic adenosine monophosphothiolate
Escherichia coli
-
37°C, pH 7.4, DNA ligation
0.0009
2,4-diamino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrile
Escherichia coli
-
-
0.00023
2,4-diamino-7-tert-butyl-4a,8a-dihydro-1,8-naphthyridine-3-carbonitrile
Escherichia coli
-
at pH 7.4 and 20°C
0.00127
2-(butylsulfanyl)adenosine
0.000158 - 0.00016
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
0.00037
2-(cyclopentyloxy)-5'-deoxyadenosine
0.01
2-amino-5-(2-hydroxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.01
2-amino-5-(2-methoxyethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0025
2-amino-5-(aminomethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-5-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0001
2-amino-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.0025
2-amino-5-phenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.001
2-amino-5-[(3R)-3-aminopyrrolidin-1-yl]-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.001
2-amino-6-(2-phenylethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.0005
2-amino-6-(hydroxymethyl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0008
2-amino-6-(propan-2-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0008
2-amino-6-benzyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-benzyl-7-chloro-5-methyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-bromo-5-(3-hydroxyazetidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0007
2-amino-6-bromo-5-(4-hydroxypiperidin-1-yl)-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0007
2-amino-6-bromo-5-[(2-hydroxyethyl)(methyl)amino]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0007
2-amino-6-bromo-5-[(3R)-3-hydroxypyrrolidin-1-yl]-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0003
2-amino-6-bromo-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-bromo-7-cyclopentyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0003
2-amino-6-bromo-7-tert-butyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.008
2-amino-6-chloro-5-ethyl-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0025
2-amino-6-cyclopentyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0004
2-amino-6-cyclopropyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0004
2-amino-6-ethenyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0006
2-amino-6-ethyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0005
2-amino-6-methyl-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.00008
2-amino-7,8-difluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.0005
2-amino-7-(trifluoromethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.0008
2-amino-7-chloro-5-methyl-6-(prop-2-en-1-yl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.001
2-amino-7-chloro-5-methyl-6-(pyridin-4-ylmethyl)-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
pH and temperature not specified in the publication
0.00004
2-amino-7-fluoro-5-oxo-5H-chromeno[2,3-b]pyridine-3-carboxamide
Escherichia coli
-
-
0.005
2-amino-7-[2-(benzyloxy)propan-2-yl]-6-bromo-1,8-naphthyridine-3-carboxamide
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.0041
2-methylthio ADP
Escherichia coli
-
37°C, pH 7.4, DNA ligation
0.0011
2-methylthio ATP
Escherichia coli
-
37°C, pH 7.4, DNA ligation
0.000036
3-(4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl)propanenitrile
Escherichia coli
-
pH and temperature not specified in the publication
0.138
3-[[[[(2R,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]oxy](hydroxy)phosphoryl]carbamoyl]-1-[5-O-(hydroxyphosphinato)-beta-D-ribofuranosyl]pyridin-1-ium
Escherichia coli
-
pH and temperature not specified in the publication
0.000019
4-([4-amino-2-[(2-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)benzoic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.00009
4-amino-2-(1,4-dioxaspiro[4.5]dec-8-yloxy)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00195
4-amino-2-(2-fluoroethoxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.01
4-amino-2-(cyclopentylamino)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.0051
4-amino-2-(cyclopentyloxy)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.0035
4-amino-2-(cyclopentylsulfanyl)-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000063
4-amino-2-(cyclopentylsulfanyl)-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00031
4-amino-2-[(1,3-difluoropropan-2-yl)oxy]-8-(2-hydroxyethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00017
4-amino-2-[(2,4-dimethylcyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000036
4-amino-2-[(2-methylcyclohexyl)oxy]-8-(1H-pyrazol-4-ylmethyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000028
4-amino-2-[(2-methylcyclohexyl)oxy]-8-[(1R,5R)-7-oxo-6-oxabicyclo[3.2.1]oct-4-yl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000041
4-amino-2-[(4-chlorocyclohexyl)oxy]-8-[(1R)-2-hydroxycyclopentyl]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.025
4-amino-6-(1H-benzimidazol-2-yl)pyridazin-3(2H)-one
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.000079
4-amino-8-(2,3-dihydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000057
4-amino-8-(2-hydroxyethyl)-2-(2-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00042
4-amino-8-(2-hydroxyethyl)-2-(4-methylcyclohexyl)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00106
4-amino-8-(2-hydroxyethyl)-2-(propan-2-yloxy)pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000028
4-amino-8-(3-fluoro-2-hydroxypropyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00002
4-amino-8-(cyclopent-3-en-1-ylmethyl)-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00035
4-amino-8-[(1R)-2-hydroxycyclopentyl]-2-[[4-(trifluoromethyl)cyclohexyl]oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000225
4-amino-8-[(1S)-2,3-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000039
4-amino-8-[(1S,2S)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00011
4-amino-8-[(2R)-2-hydroxycyclohexyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00019
4-amino-8-[(2R)-2-hydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000076
4-amino-8-[(2S,5S)-2,5-dihydroxycyclopentyl]-2-[(4-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000068
4-amino-8-[(3,4-dihydroxycyclopentyl)methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00004
4-amino-8-[[(2R)-2-hydroxycyclopentyl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.000038
4-amino-8-[[(4S)-4-hydroxytetrahydrofuran-3-yl]methyl]-2-[(2-methylcyclohexyl)oxy]pyrido[2,3-d]pyrimidin-5(8H)-one
Escherichia coli
-
pH and temperature not specified in the publication
0.00018
5'-deoxy-2-(spiro[2.2]pent-1-ylmethoxy)adenosine
Escherichia coli
-
pH 7.5, 22°C
0.00018
5'-deoxy-2-[(spiro[2.2]pentan-1-yl)methoxy]adenosine
Escherichia coli
-
pH and temperature not specified in the publication
0.00032
5'-deoxy-2-[(trans-4-methylcyclohexyl)oxy]adenosine
Escherichia coli
-
pH and temperature not specified in the publication
0.00032
5'-deoxy-5'-fluoro-2-[(trans-4-methylcyclohexyl)oxy]adenosine
Escherichia coli
-
pH 7.5, 22°C
0.00049
5-([4-amino-2-[(4-methylcyclohexyl)oxy]-5-oxopyrido[2,3-d]pyrimidin-8(5H)-yl]methyl)thiophene-2-carboxylic acid
Escherichia coli
-
pH and temperature not specified in the publication
0.000077
7-amino-2-tert-butyl-4-(1H-pyrrol-2-yl)-4a,8a-dihydropyrido[2,3-d]pyrimidine-6-carboxamide
Escherichia coli
-
at pH 7.4 and 20°C
0.025
7-methoxy-6-methylpteridine-2,4-diamine
Escherichia coli
-
pH and temperature not specified in the publication
0.053
Chloroquine
Escherichia coli
-
-
0.063
hydroxychloroquine
Escherichia coli
-
-
0.0018
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-6-yl]sebacamide
Escherichia coli
-
-
0.009
N,N'-bis[4-((4-(diethylamino)-1-methylbutyl)amino)-quinoline-8-yl]adipamide
Escherichia coli
-
-
0.0015
Quinacrine
Escherichia coli
-
-
0.0015
Sp-3',5'-cyclic adenosine monophosphothiolate
Escherichia coli
-
37°C, pH 7.4, DNA ligation
0.005
tert-butyl 3-(7-amino-3-bromo-6-carbamoyl-1,8-naphthyridin-2-yl)azetidine-1-carboxylate
Escherichia coli
-
value above, pH and temperature not specified in the publication
0.00127
2-(butylsulfanyl)adenosine
Escherichia coli
-
pH 7.5, 22°C
0.00127
2-(butylsulfanyl)adenosine
Escherichia coli
-
pH and temperature not specified in the publication
0.000158
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
Escherichia coli
-
pH 7.5, 22°C
0.00016
2-(cyclobutylmethoxy)-5'-deoxy-5'-fluoroadenosine
Escherichia coli
-
pH and temperature not specified in the publication
0.00037
2-(cyclopentyloxy)-5'-deoxyadenosine
Escherichia coli
-
pH 7.5, 22°C
0.00037
2-(cyclopentyloxy)-5'-deoxyadenosine
Escherichia coli
-
pH and temperature not specified in the publication
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D450A
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks
D452A
mutant with 68% of wild type activity
G489A
the mutant retains 25% of wild type activity
G489D
mutant with 0.8% of wild type activity
G489V
mutant with 3.2% of wild type activity
G521A
the mutant is 50fold less active than wild type LigA
G521D
mutant with less than 0.1% of wild type function
G521V
mutant with less than 0.1% of wild type function
G553A
mutant with 38% of wild type activity
G553D
mutant with 2.6% of wild type activity
G553V
mutant with 0.9% of wild type activity
I384A
the mutant is defective for nick sealing, retaining 4% of wild type activity
K627A
mutant with 40% of wild type activity
K634A/K635A
mutant with 44% of wild type activity
K648A
mutant with 37% of wild type activity
K651A
mutant with 35% of wild type activity
N355A
mutant with 30% of wild type activity
Q330A
the mutation has no apparent effect on nick sealing acivity
Q386A
mutant with 30% of wild type activity
R333A
the oligonucleotide-binding domain mutation strongly suppresses DNA relaxation
R379A
the oligonucleotide-binding domain mutation strongly suppresses DNA relaxation
R487A
mutant with 6% of wild type activity
R510A
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks
R614A
mutant with 14% of wild type activity
V383A
the mutant is defective for nick sealing, retaining 4% of wild type activity
C408A
-
inactive, unable to complement DNA-ligase-deficient yeast
C411A
-
inactive, unable to complement DNA-ligase-deficient yeast
C426A
-
no effect in vivo, 7% of wild type activity
C432A
-
inactive, unable to complement DNA-ligase-deficient yeast
D117A
-
inactive in nick-joining, unable to complement DNA-ligase-deficient yeast
D117E
-
3% of ligase activity of wild-type enzyme, lethal mutation
D117N
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
D138A
-
37% of ligase activity of wild-type enzyme
D283A
-
57% of ligase activity of wild-type enzyme
D285E
-
4% of ligase activity of wild-type enzyme, lethal mutation
D285N
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
D32A
-
traces of nick joining activity
D32E
-
traces of nick joining activity
D32N
-
9% of nick joining activity, strongly reduced self-adenylation
D36A
-
traces of nick joining activity
D36E
-
4% of nick joining activity, strongly reduced self-adenylation
D36N
-
12% of nick joining activity, strongly reduced self-adenylation
DELTA1-592
-
fragment corresponding to BRCT domain binds to a wider range of substrates compared to full-length LigA
DELTA593-671
-
mutant lacking the BRCT domain, 3fold reduced in vitro ligation activity and reduced DNA binding
E10A
-
90% of nick joining activity
E113A
-
no effect in vivo, increased Km for NAD+, 40% of wild type activity
E143A
-
52% of ligase activity of wild-type enzyme
E173A
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
E173D
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
E173Q
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
E319A
-
no effect in vivo, 69% of wild type activity
G118A
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
G172A
-
36% of ligase activity of wild-type enzyme
G286A
-
14% of ligase activity of wild-type enzyme
H23A
-
10% of nick joining activity
H23Y
-
88% of nick joining activity
K115A
-
inactive in nick-joining, unable to complement DNA-ligase-deficient yeast
K115Q
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
K115R
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
K290A
-
13% of ligase activity of wild-type enzyme, lethal mutation
K314A [DV1]
-
accumulation of the DNA-adenylate intermediate, loss of function in vivo, strongly reduced activity
K314Q
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
K314R
-
5% of ligase activity of wild-type enzyme, lethal mutation
L119A
-
110% of ligase activity of wild-type enzyme
L15F
-
at 20°C mutant enzyme L15F has 20fold lower ligation activity in vitro, and its activity is reduced at 42°C, resulting in 60fold lower ligation activity than wild-type LigA
N198A
-
26% of ligase activity of wild-type enzyme
Q318A
-
no effect in vivo, 80% of wild type activity
R200A
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
R200K
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
R200Q
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
R208A
-
2% of ligase activity of wild-type enzyme, lethal mutation
R208K
-
1.2% of ligase activity of wild-type enzyme, lethal mutation
R208Q
-
less than 0.1% of ligase activity of wild-type enzyme, lethal mutation
R277K
-
5.6% of ligase activity of wild-type enzyme, themperature-sensitive mutation, rate of isolated sealing step is 4% of the activity of wild-type enzyme
R277Q
-
1.8% of ligase activity of wild-type enzyme, lethal mutation, rate of isolated sealing step is 10% of the activity of wild-type enzyme
V288A
-
74% of ligase activity of wild-type enzyme
Y225A
-
no effect in vivo, increased Km for NAD+, 25% of wild type activity
Y22A
-
traces of nick joining activity, strongly reduced self-adenylation
Y22S
-
9% of nick joining activity
Y35A
-
2% of nick joining activity, strongly reduced self-adenylation
Y35S
-
23% of nick joining activity
D551A
mutant with 120% of wild type activity
D551A
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks
E519A
mutant with 71% of wild type activity
E519A
the helix-hairpin-helix domain mutant retains DNA relaxation function, concordant with the ability to effectively ligate nicks
DELTA1-38
-
inactive, unable to complement DNA-ligase-deficient yeast
DELTA1-38
-
nick joining activity abolished, no self adenylation, active with pre-adenylated nick
DELTA1-78
-
inactive, unable to complement DNA-ligase-deficient yeast
DELTA1-78
-
nick joining activity abolished, no self adenylation, active with pre-adenylated nick
R277A
-
1.1% of ligase activity of wild-type enzyme, lethal mutation
R277A
-
rate of isolated sealing step is 20% of the activity of wild-type enzyme
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DNA ligases
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1982
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A DNA ligase from the psychrophile Pseudoalteromonas haloplanktis gives insights into the adaptation of proteins to low temperatures
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Molecular cloning and characterization of thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic bacterium
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Conserved residues in domain Ia are required for the reaction of Escherichia coli DNA ligase with NAD+
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Specific and potent inhibition of NAD+-dependent DNA ligase by pyridochromanones
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2003
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Mutational analysis of Escherichia coli DNA ligase identifies amino acids required for nick-ligation in vitro and for in vivo complementation of the growth of yeast cells deleted for CDC9 and LIG4
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1999
Escherichia coli
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A second NAD+-dependent DNA ligase (LigB) in Escherichia coli
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2001
Escherichia coli
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NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor
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2003
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Tethered DNA hairpins facilitate electrochemical detection of DNA ligation
Analyst
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Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA)
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Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA)
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Characterization of a temperature-sensitive DNA ligase from Escherichia coli
Microbiology
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A high-throughput assay for the adenylation reaction of bacterial DNA ligase
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Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate
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26
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Label-free electrochemical monitoring of DNA ligase activity
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Simultaneous identification of point mutations via DNA ligase-mediated gold nanoparticle assembly
Analyst
133
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DNA and RNA ligases: structural variations and shared mechanisms
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NAD+-dependent DNA ligase: a novel target waiting for the right inhibitor
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Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA and E. coli DNA ligase
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22
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25
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Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA)
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44
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Escherichia coli
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45
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13
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