Information on EC 6.4.1.8 - acetophenone carboxylase

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The expected taxonomic range for this enzyme is: Aromatoleum aromaticum

EC NUMBER
COMMENTARY hide
6.4.1.8
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RECOMMENDED NAME
GeneOntology No.
acetophenone carboxylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 ATP + acetophenone + HCO3- + H2O + H+ = 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ethylbenzene degradation
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ethylbenzene degradation (anaerobic)
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
acetophenone:carbon-dioxide ligase (ADP-forming)
The enzyme is involved in anaerobic degradation of ethylbenzene. No activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain EbN1
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Manually annotated by BRENDA team
strain EbN1
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ATP + 4-acetylpyridine + HCO3- + H2O + H+
2 ADP + 2 phosphate + 2-oxo-3-(pyridin-4-yl)propanoate
show the reaction diagram
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
show the reaction diagram
2 ATP + propiophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 4-oxo-4-phenylbutanoate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ATP + acetophenone + HCO3- + H2O + H+
2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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20 mM, required for optimal activity
Mg2+
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the enzyme is dependent on the presence of Mg2+ or Mn2+
Mn2+
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the enzyme is dependent on the presence of Mg2+ or Mn2+
NH4+
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40 mM, required for optimal activity
Zn2+
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the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta,gamma-Imido-ATP
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2 mM, complete inhibition of acetophenone carboxylase activity, non-competitive inhibition
Carbamoyl phosphate
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2 mM: 50% inhibition of ATPase activity. 8 mM: 50% inhibition of carboxylating activity
Mg2+
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20 mM: 50% inhibition
Mn2+
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10 mM: 50% inhibition
Ni2+
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1 mM: 50% inhibition of ATPase activity. 2.5 mM: 50% inhibition of carboxylation activity
Zn2+
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the enzyme contains 1 Zn2+ ion per alphabetagammadelta core complex. In spite of the presence of Zn in the enzyme, it is strongly inhibited by Zn2+ ions. 0.2 mM ZnCl2: 50% inhibition of ATPase activity and carboxylation activity
additional information
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acetophenone carboxylation activity in cell extracts is not sensitive to avidin, indicating that the enzyme is independent of biotin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
acetophenone
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pH 6.5, 30°C
0.5
ATP
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pH 6.5, 30°C
0.54
HCO3-
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pH 6.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.051
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pH 6.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aromatoleum aromaticum (strain EbN1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
34000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
40000
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apcE gene product, gel filtration
70000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
75000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
87000
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1 * 70000 (alpha) + 1 * 15000 (beta) + 1 * 87000 (gamma) + 1 * 75000 (delta) + 1 * 34000 (epsilon), the genes (apcABCDE) of the five subunits form an apparent operon
485000
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apcABCD gene products, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
during purification, acetophenone carboxylase dissociates into inactive subcomplexes consisting of the 70-, 15-, 87-, and 75-kDa subunits (apcABCD gene products) and the 34-kDa subunit (apcE gene product), respectively. Acetophenone carboxylase activity is restored by mixing the purified subcomplexes
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
heterologous expression of the apcEC-His6 subunit in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells
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acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells; the enzyme is synthesized at high levels in cells grown on ethylbenzene or acetophenone, but not in cells grown on benzoate
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the enzyme is synthesized at high levels in cells grown on ethylbenzene or acetophenone, but not in cells grown on benzoate
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