Information on EC 6.4.1.5 - geranoyl-CoA carboxylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
6.4.1.5
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RECOMMENDED NAME
GeneOntology No.
geranoyl-CoA carboxylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + geranoyl-CoA + HCO3- = ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cis-genanyl-CoA degradation
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Geraniol degradation
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SYSTEMATIC NAME
IUBMB Comments
geranoyl-CoA:carbon-dioxide ligase (ADP-forming)
A biotinyl-protein. Also carboxylates dimethylpropenoyl-CoA and farnesoyl-CoA.
CAS REGISTRY NUMBER
COMMENTARY hide
37324-35-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
inducible enzyme
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-methylcrotonyl-CoA + HCO3-
?
show the reaction diagram
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4-8% of the activity relative to (Z)-geranoyl-CoA
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ATP + 3-methylcrotonyl-CoA + HCO3-
ADP + phosphate + 3-methylglutaconyl-CoA
show the reaction diagram
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geranoyl-CoA is preferred over 3-methylcrotonyl-CoA as substrate
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?
ATP + dimethylacryl-CoA + HCO3-
?
show the reaction diagram
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ATP + ethylcrotonyl-CoA + HCO3-
?
show the reaction diagram
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cis-ethylcrotonyl-CoA and trans-ethylcrotonyl-CoA
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ATP + farnesyl-CoA + HCO3-
?
show the reaction diagram
ATP + geranoyl-CoA + HCO3-
?
show the reaction diagram
ATP + geranoyl-CoA + HCO3-
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + geranoyl-CoA + HCO3-
?
show the reaction diagram
ATP + geranoyl-CoA + HCO3-
ADP + phosphate + 3-(4-methylpent-3-en-1-yl)pent-2-enedioyl-CoA
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
biotin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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absolute requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Avidin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
3-methylcrotonyl-CoA
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pH 8.5, 37C
0.008 - 0.01
ATP
0.064
geranoyl-CoA
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0.58 - 0.6
HCO3-
additional information
additional information
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GCCase shows sigmoidal kinetic behavior for geranoyl-CoA and bicarbonate but shows Michaelis-Menten kinetics for 3-methylcrotonyl-CoA and the cosubstrate ATP. The apparent K0.5 values of GCCase are 8.8 microM and 1.2 microM for geranoyl-CoA and bicarbonate, respectively
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.138
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additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.3
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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active over a broad pH-range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74000
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x * 74000, biotinylated subunit AtuF, x * 63000, subunit AtuC
122000
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x * 122000, biotin-containing subunit, SDS-PAGE, Western blot analysis
520000 - 580000
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gel filtration, native PAGE
550000
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about
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
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4 * 63000 + 4 * 75000, biotin-containing subunits, SDS-PAGE
additional information
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biotin-containing subunit of 122 kDa
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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50% loss of activity after 1 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, protein suspension with 60% saturation of NH4+, stable for several weeks
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-80C, stable for several months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
184fold, from leaves
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reconstitution of functional enzyme after coexpression and copurification of the subunit genes AtuC and AtuF
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
reconstitution of functional enzyme after coexpression and copurification of the subunit genes AtuC and AtuF
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