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EC Tree
IUBMB Comments A biotinyl-protein. The yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3. Previously also listed as EC 3.5.1.45. The enzyme from the prokaryotic bacterium Oleomonas sagaranensis can also use acetamide and formamide as substrates .
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
urea amidolyase, dur1,2, urea carboxylase, urea amido-lyase, atp-urea amidolyase,
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DUR1,2
a 1800 amino acid protein with a 600 amino acid amidase domain fused at the N-terminus
ATP:urea amidolyase
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Urea carboxylase (hydrolysing)
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Urease (ATP-hydrolysing)
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ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate
a biotinyl-protein, the yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3, previously also listed as EC 3.5.1.45
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urea:carbon-dioxide ligase (ADP-forming)
A biotinyl-protein. The yeast enzyme (but not that from green algae) also catalyses the reaction of EC 3.5.1.54 allophanate hydrolase, thus bringing about the hydrolysis of urea to CO2 and NH3. Previously also listed as EC 3.5.1.45. The enzyme from the prokaryotic bacterium Oleomonas sagaranensis can also use acetamide and formamide as substrates [4].
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ATP + urea + CO2
ADP + phosphate + urea-1-carboxylate
multifunctional enzyme, catalyzes carboxylation of urea with urea carboxylase, EC 6.3.4.6, and hydrolysis of allophanate with allophanate hydrolase, EC 3.5.1.54
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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urea + H2O + ATP
CO2 + NH3 + ADP + phosphate
in the presence of enzyme, ATP, bicarbonate, magnesium, and potassium ions, ammonium ion is produced from human serum urea
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ATP + 2-chloroacetamide + HCO3-
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ATP + acetamide + HCO3-
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ATP + formamide + HCO3-
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ATP + formylurea + HCO3-
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ATP + hydantoic acid + HCO3-
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ATP + hydroxyurea + HCO3-
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ATP + N-methylurea + HCO3-
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ATP + urea + HCO3-
ADP + phosphate + allophanate
ATP + urethane + HCO3-
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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ATP + urea + HCO3-
ADP + phosphate + allophanate
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HCO3-
requirement, hydrolysis of urea
K+
requirement, hydrolysis of urea
Mg2+
requirement, hydrolysis of urea
additional information
requires divalent and monovalent ions
additional information
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requires divalent and monovalent ions
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Ca2+
strong, Ca2+ affects the enzyme-ATP-Mg complex by the time of urea carboxylation
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ATP
ATP-dependent cleavage of urea
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additional information
additional information
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0.1
Urea
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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7.5 - 8
hydrolysis of urea
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SwissProt
brenda
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201665
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x * 201665, calculation from nucleotide sequence
204000
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x * 204000, SDS-PAGE, amino acid composition
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x * 204000, SDS-PAGE, amino acid composition
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x * 201665, calculation from nucleotide sequence
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diagnostics
enzymatic assay for measurement of serum urea nitrogen using urea amidolyase
analysis
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enzymatic assay with urea amidolyase for determination of potassium in serum
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Whitney, P.A.; Cooper, T.G.
Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae
J. Biol. Chem.
247
1349-1353
1972
Saccharomyces cerevisiae
brenda
Roon, R.J.; Levenberg, B.
Urea amidolyase. I. Properties of the enzyme from Candida utilis
J. Biol. Chem.
247
4107-4113
1972
Saccharomyces cerevisiae, Debaryomyces subglobosus, Cyberlindnera jadinii, Chlamydomonas reinhardtii, Pseudochlorella pringsheimii, Auxenochlorella pyrenoidosa
brenda
Whitney, P.A.; Cooper, T.G.
Urea carboxylase and allophanate hydrolase: two components of a multienzyme complex in Saccharomyces cerevisiae
Biochem. Biophys. Res. Commun.
49
45-51
1972
Saccharomyces cerevisiae
brenda
Roon, R.J.; Hampshire, J.; Levenberg, B.
Urea amidolyase. The involvement of biotin in urea cleavage
J. Biol. Chem.
247
7539-7545
1972
Saccharomyces cerevisiae, Cyberlindnera jadinii
brenda
Whitney, P.A.; Cooper, T.
Urea carboxylase from Saccharomyces cerevisiae. Evidence for a minimal two-step reaction sequence
J. Biol. Chem.
248
325-330
1973
Saccharomyces cerevisiae
brenda
Sumrada, R.A.; Cooper, T.G.
Urea carboxylase and allophanate hydrolase are components of a multifunctional protein in yeast
J. Biol. Chem.
257
9119-9127
1982
Saccharomyces cerevisiae
brenda
Kimura, S.; Iyama, S.; Yamaguchi, Y.; Hayashi, S.; Fushimi, R.; Amino, N.
New enzymatic assay with urea amidolyase for determining potassium in serum
Ann. Clin. Biochem.
34
384-388
1997
Saccharomyces cerevisiae
brenda
Genbauffe, F.S.; Cooper, T.G.
The urea amidolyase (DUR1,2) gene of Saccharomyces cerevisiae
DNA Seq.
2
19-32
1991
Saccharomyces cerevisiae
brenda
Kimura, S.; Iyama, S.; Yamaguchi, Y.; Kanakura, Y.
New enzymatic assay for serum urea nitrogen using urea amidolyase
J. Clin. Lab. Anal.
17
52-56
2003
Saccharomyces cerevisiae (P32528), Saccharomyces cerevisiae
brenda
Navarathna, D.H.; Harris, S.D.; Roberts, D.D.; Nickerson, K.W.
Evolutionary aspects of urea utilization by fungi
FEMS Yeast Res.
10
209-213
2010
Eremothecium gossypii, Aspergillus fumigatus, Aspergillus nidulans, Candida albicans, [Candida] glabrata, Fusarium graminearum, Kluyveromyces lactis, Pyricularia grisea, Clavispora lusitaniae, Parastagonospora nodorum, Saccharomyces cerevisiae (P32528)
brenda