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Information on EC 6.3.4.3 - formate-tetrahydrofolate ligase and Organism(s) Methylorubrum extorquens and UniProt Accession Q83WS0

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EC Tree
     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.3 formate-tetrahydrofolate ligase
IUBMB Comments
In eukaryotes occurs as a trifunctional enzyme also having methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) and methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) activity.
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This record set is specific for:
Methylorubrum extorquens
UNIPROT: Q83WS0
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Word Map
The taxonomic range for the selected organisms is: Methylorubrum extorquens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
fthfs, formyltetrahydrofolate synthetase, c1-thf synthase, mthfd, 10-formyltetrahydrofolate synthetase, c1-tetrahydrofolate synthase, 10-formyl-thf synthetase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase 1-like, n10-formyltetrahydrofolate synthetase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10-Formyltetrahydrofolate synthetase
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FHS
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formate-tetrahydrofolate ligase
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formate:tetrahydrofolate ligase (ADP-forming)
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-
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Formyl-THF synthetase
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Formyltetrahydrofolate synthetase
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FTHFS
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Synthetase, formyl tetrahydrofolate
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Tetrahydrofolate formylase
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Tetrahydrofolic formylase
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THFS
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formylation
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
formate:tetrahydrofolate ligase (ADP-forming)
In eukaryotes occurs as a trifunctional enzyme also having methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) and methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) activity.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-66-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + formate + tetrahydrofolate
ADP + phosphate + 10-formyltetrahydrofolate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + formate + tetrahydrofolate
ADP + phosphate + 10-formyltetrahydrofolate
show the reaction diagram
FtfL activity is required for methylotrophic growth
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
ATP
30°C, pH 8.0
22
formate
30°C, pH 8.0
0.8
tetrahydrofolate
30°C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
gel filtration
60000
4 * 60000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 60000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Phenyl Sepharose, Source15Q, hydroxyapatite, Resource Q
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marx, C.J.; Laukel, M.; Vorholt, J.A.; Lidstrom, M.E.
Purification of the formate-tetrahydrofolate ligase from Methylobacterium extorquens AM1 and demonstration of its requirement for methylotrophic growth
J. Bacteriol.
185
7169-7175
2003
Methylorubrum extorquens (Q83WS0), Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 (Q83WS0)
Manually annotated by BRENDA team