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EC Tree
The taxonomic range for the selected organisms is: Escherichia coli The enzyme appears in selected viruses and cellular organisms
Synonyms
glycinamide ribonucleotide synthetase, gar synthetase, gar-syn, phosphoribosylglycinamide synthetase, phosphoribosylamine-glycine ligase, glycinamide ribotide synthetase, phosphoribosylglycineamide synthetase, 5'-phosphoribosylglycinamide synthetase, prg synthetase, trifunctional purine biosynthetic protein adenosine-3,
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2-Amino-N-ribosylacetamide 5'-phosphate kinosynthase
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5'-Phosphoribosylglycinamide synthetase
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Glycinamide ribonucleotide synthetase
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Glycineamide ribonucleotide synthetase
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Phosphoribosylglycinamide synthetase
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Phosphoribosylglycineamide synthetase
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Synthetase, phosphoribosylglycinamide
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ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
mechanism
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5-phospho-D-ribosylamine:glycine ligase (ADP-forming)
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ATP + 5-phospho-D-ribosylamine + Gly
ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
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ATP + 5-phospho-D-ribosylamine + glycine
ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
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ATP in form of MgATP2-
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ATP + 5-phosphoribosylamine + Gly
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sequential ordered mechanism of substrate binding and product release in which 5-phosphoribosylamine binds first followed by MgATP2- and then Gly. Phosphate leaves first followed by loss of MgADP- and finally phosphoribosylglycinamide
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ATP + 5-phosphoribosylamine + Gly
ADP + phosphate + 5-phosphoribosylglycinamide
ATP + carbocyclic 5-phosphoribosylamine + Gly
ADP + phosphate + carbocyclic glycinamide ribonucleotide
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r
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additional information
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ATP + 5-phosphoribosylamine + Gly
ADP + phosphate + 5-phosphoribosylglycinamide
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ATP + 5-phosphoribosylamine + Gly
ADP + phosphate + 5-phosphoribosylglycinamide
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r
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ATP + 5-phosphoribosylamine + Gly
ADP + phosphate + 5-phosphoribosylglycinamide
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additional information
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second step in de novo purine biosynthesis pathway
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additional information
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second step in de novo purine biosynthesis pathway
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additional information
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requirement for ATP and a substrate with a carboxyl group or its relatives, transfer of a phosphate group from ATP to the carboxyl group or its relatives
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additional information
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second step in de novo purine biosynthesis pathway
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ATP + 5-phosphoribosylamine + Gly
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sequential ordered mechanism of substrate binding and product release in which 5-phosphoribosylamine binds first followed by MgATP2- and then Gly. Phosphate leaves first followed by loss of MgADP- and finally phosphoribosylglycinamide
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additional information
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additional information
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second step in de novo purine biosynthesis pathway
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additional information
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second step in de novo purine biosynthesis pathway
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additional information
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second step in de novo purine biosynthesis pathway
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0.07
5'-phosphoribosylamine
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19
methionine-substituted enzyme
2.3
selenomethionine-substituted enzyme
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18
no detectable activity at this temperature
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Uniprot
brenda
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48240
calculated from amino acid sequence
45945
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x * 45945, calculation from nucleotide sequence
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x * 45945, calculation from nucleotide sequence
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selenomethionine incorporated enzyme, hanging drop vapor diffusion method
selenomethionine incorporated enzyme, hanging drop vapor diffusion method
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expressed as the selenomethionine incorporated protein
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Cheng, Y.S.; Murray, M.; Schendel, F.; Otvos, J.; Wehrli, S.; Stubbe, J.
Chemical characterization of phosphoribosylamine, a substrate for newly discovered trifunctional protein containing glycinamide ribonucleotide synthetase activity
Adv. Enzyme Regul.
26
319-333
1987
Gallus gallus, Drosophila melanogaster, Escherichia coli, Schizosaccharomyces pombe
brenda
Shen, Y.; Rudolph, J.M.; Stern, J.R.; Stubbe, J.
Glycinamide ribonucleotide synthetase from Escherichia coli: cloning, overproduction, sequencing, isolation, and characterization
Biochemistry
29
218-227
1990
Escherichia coli
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Liu, D.S.; Caperelli, C.A.
Carbocyclic substrates for de novo purine biosynthesis
J. Biol. Chem.
266
16699-16702
1991
Escherichia coli, eukaryota
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Weaver, T.M.; Wang, W.; Ealick, S.E.
Purification, crystallization and preliminary X-ray diffraction data from selenomethionine glycinamide ribonucleotide synthetase
Acta Crystallogr. Sect. D
55
518-521
1999
Escherichia coli
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Wang, W.; Kappock, T.J.; Stubbe, J.; Ealick, S.E.
X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli
Biochemistry
37
15647-15662
1998
Escherichia coli (P15640), Escherichia coli
brenda
Kanai, S.; Toh, H.
Identification of new members of the GS ADP-forming family from the de novo purine biosynthesis pathway
J. Mol. Evol.
48
482-492
1999
Synechocystis sp., Arabidopsis thaliana, Archaeoglobus fulgidus, Bacillus subtilis, Saccharomyces cerevisiae, Yarrowia lipolytica, Gallus gallus, Drosophila melanogaster, Drosophila pseudoobscura, Escherichia coli, Haemophilus influenzae, Homo sapiens, Methanothermobacter thermautotrophicus, Methanocaldococcus jannaschii, Mus musculus, Pyrococcus horikoshii, Salmonella enterica subsp. enterica serovar Typhimurium, Schizosaccharomyces pombe, Chironomus tentans, Pyrococcus horikoshii OT-3
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