Information on EC 6.3.3.5 - O-ureido-D-serine cyclo-ligase

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The expected taxonomic range for this enzyme is: Streptomyces lavendulae

EC NUMBER
COMMENTARY hide
6.3.3.5
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RECOMMENDED NAME
GeneOntology No.
O-ureido-D-serine cyclo-ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
O-ureido-D-serine + ATP + H2O = D-cycloserine + CO2 + NH3 + ADP + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-cycloserine biosynthesis
Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
O-ureido-D-serine cyclo-ligase (D-cycloserine-forming)
The enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-aminooxy-D-alanine + ATP + H2O
D-cycloserine + ?
show the reaction diagram
D-2,4-diaminobutyrate + ATP + H2O
?
show the reaction diagram
D-homocysteine + ATP + H2O
?
show the reaction diagram
D-homoserine + ATP + H2O
?
show the reaction diagram
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-
-
-
?
D-ornithine + ATP + H2O
?
show the reaction diagram
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-
-
-
?
O-ureido-D-serine + ATP + H2O
D-cycloserine + CO2 + ammonia + ADP + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O-ureido-D-serine + ATP + H2O
D-cycloserine + CO2 + ammonia + ADP + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023 - 0.073
ATP
19
D-homocysteine
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pH 8.0, 30°C
10
O-ureido-D-serine
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pH 8.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
860
D-homocysteine
-
pH 8.0, 30°C
2200
O-ureido-D-serine
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pH 8.0, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14
beta-aminooxy-D-alanine
-
pH 8.0, 30°C
2.1
D-2,4-diaminobutyrate
-
pH 8.0, 30°C
45
D-homocysteine
-
pH 8.0, 30°C
6.1
D-homoserine
-
pH 8.0, 30°C
0.67
D-ornithine
-
pH 8.0, 30°C
210
O-ureido-D-serine
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pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
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gel filtration
34000
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1 * 34000, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C-terminally His6-tagged enzyme is overexpression in Escherichia coli
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