Information on EC 6.3.2.B4 - polyglycylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.2.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
polyglycylase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + alpha-tubulin + glycine = glycyl-alpha-tubulin + ADP + phosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-tubulin:glycine ligase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 14-3-3 protein + glycine
glycyl-14-3-3 protein + ADP + phosphate
show the reaction diagram
ATP + alpha-tubulin + glycine
glycyl-alpha-tubulin + ADP + phosphate
show the reaction diagram
ATP + nucleosome assembly protein 1 + glycine
glycylated nucleosome assembly protein 1 + ADP + phosphate
show the reaction diagram
-
i.e. NAP1, glycylation at Glu residues at 359 and 360
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 14-3-3 protein + glycine
glycyl-14-3-3 protein + ADP + phosphate
show the reaction diagram
ATP + alpha-tubulin + glycine
glycyl-alpha-tubulin + ADP + phosphate
show the reaction diagram
ATP + nucleosome assembly protein 1 + glycine
glycylated nucleosome assembly protein 1 + ADP + phosphate
show the reaction diagram
-
i.e. NAP1, glycylation at Glu residues at 359 and 360
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immunohistochemic enzyme determination
Manually annotated by BRENDA team
additional information
-
tissue distribution study, overview
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant GST-tagged TTLL10 from Spodoptera frugiperda Sf9 cells, co-expression of EGFP-tagged TTLL10 with FLAG-tagged NAP1 in HEK-293 cells
-
recombinant N-terminally FLAG-tagged enzyme from Gardia duodenalis trophozoites by FLAG affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of GST-tagged TTLL10 in Spodoptera frugiperda Sf9 cells, co-expression of EGFP-tagged TTLL10 with FLAG-tagged NAP1 in HEK-293 cells
-
gene gtll3, phylogenetic analysis, RNA reverse transcription and real-time quantitative PCR expression analysis, expression of the N-terminally FLAG-tagged enzyme, under the constitutive promoter of the Gardia duodenalis alpha-tubulin gene, in Gardia duodenalis transgenic trophozoites, subcloning in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E499V
-
site-directed mutagenesis, inactive active site mutant