Information on EC 6.3.2.B2 - murein peptide ligase

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The expected taxonomic range for this enzyme is: Psychrobacter arcticus

EC NUMBER
COMMENTARY hide
6.3.2.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
murein peptide ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-N-acetylmuramate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
L-alanyl-gamma-D-glutamyl-meso-diaminopimelate:UDP-N-acetylmuramate ligase (ADP-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene mpl
UniProt
Manually annotated by BRENDA team
gene mpl
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
show the reaction diagram
ATP + L-alanyl-gamma-D-glutamate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamate
show the reaction diagram
ATP + L-alanyl-gamma-D-glutamyl-L-lysine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine
show the reaction diagram
ATP + L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-L-lysyl-D-alanine
show the reaction diagram
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-
-
?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
show the reaction diagram
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
show the reaction diagram
-
-
-
?
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + UDP-MurNAc
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0117
L-alanine
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.023
L-alanyl-gamma-D-glutamate
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0042
L-alanyl-gamma-D-glutamyl-L-lysine
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.0017
L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
4.33
L-alanyl-gamma-D-glutamyl-meso-diaminopimelate
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.098
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
0.088
L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanyl-D-alanine
Psychrobacter arcticus
Q4FVQ2
pH 8.4, temperature not specified in the publication, recombinant His-tagged enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
57187
x * 57187, His-tagged enzyme, sequence calculation, x * 57000, recombinant His-tagged enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified enzyme, X-ray diffraction structure determination and analysis at 1.65 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain MLD2502 by nickel and cobalt affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene mpl, chromosomal mpl gene, DNA and mino acid sequence determination and analysis, expression of His-tagged enzyme in an Escherichia coli chromosomal mpl gene-deficient mutant strain MLD2502