Information on EC 6.3.2.B19 - [LysW]-C-terminal-L-glutamate ligase

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The expected taxonomic range for this enzyme is: Sulfolobus

EC NUMBER
COMMENTARY hide
6.3.2.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
[LysW]-C-terminal-L-glutamate ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate = ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
[LysW]-C-terminal-L-glutamate:L-glutamate ligase (ADP-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31534
4 * 31534, calculated from sequence
31715
x * 31715, calculated from sequence
126200
mass spectrometry
146000
analytical ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
X-ray diffraction with resolution of 1.8 A of the enzyme complexed with LysW, L-glutamate, ADP, Mg2+, Zn2+ and sulfate. X-ray diffraction with resolution of 1.87 A of the enzyme complexed with ADP
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli