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ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
-
-
-
?
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
?
key role in peptidoglycan biosynthesis
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
ATP + 1-phospho-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + 1-phospho-N-acetylmuramoyl-L-Ala-D-Glu
-
-
-
-
?
ATP + dihydrouridine 5'-diphosphate-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + dihydrouridine 5'-diphosphate-N-acetylmuramoyl-L-Ala-D-Glu
-
-
-
-
?
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate
-
ATP in form of MgATP2-
-
-
?
ATP + UDP-N-acetylmuramate-L-alanine
adenosine 5'-tetraphosphate
-
-
-
?
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
ATP + UDP-N-acetylmuramoyl-L-Ala + (+/-)trans-1-amino-3-carboxy-cyclohexanecarboxylic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-(+/-)trans-1-amino-3-carboxy-cyclohexanecarboxylic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + (+/-)trans-1-amino-3-carboxy-cyclopentanecarboxylic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-(+/-)trans-1-amino-3-carboxy-cyclopentanecarboxylic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + D-erythro-3-methylglutamic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-erythro-3-methylglutamic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + D-erythro-4-methylglutamic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-erythro-4-methylglutamic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + DL-homocysteic acid
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-DL-homocysteic acid
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
-
-
-
?
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
additional information
?
-
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
highly stereospecific for D-Glu
-
-
?
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
-
-
?
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
-
r
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
?
-
key role in peptidoglycan biosynthesis
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu
?
-
activity is not in excess in the cell under normal growth conditions, but its amount is adjusted to the requirements of peptidoglycan synthesis
-
-
?
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
-
-
-
?
ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
MurD is essential for cytoplasmic bacterial cell wall biosynthesis
-
-
?
additional information
?
-
enzyme MurD catalyzes the addition of D-glutamic acid to UDP-MurNAc-L-Ala in the presence of ATP
-
-
?
additional information
?
-
-
structure-function analysis by hybrid quantum mechanical/molecular mechanical replica path method, three possible reaction pathways via tetrahedral intermediate, overview
-
-
?
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(2R)-2-[((3-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
-
(2R)-2-[((4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
-
(2R)-2-[[(7-(2-ethoxy-2-oxoethoxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
NMR and molecular dynamics analysis
(2R)-2-[[(7-(3-phenylpropoxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
NMR and molecular dynamics analysis
(2R)-2-[[(7-(4-cyanobenzyloxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
(2R)-2-[[(7-benzyloxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
calculation of binding free energies. Main driving force for binding are non-polar van der Waals-interactions
(2R)-2-[[(7-butoxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
NMR and molecular dynamics analysis
(2R)-2-[[(7-pentoxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
calculation of binding free energies. Main driving force for binding are non-polar van der Waals-interactions
(2S)-2-[((4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
-
(R,Z)-2-(3-((1-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)formamido)methyl)benzamido)pentanedioic acid
-
(R,Z)-2-(3-((2-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)acetamido)methyl)benzamido)pentanedioic acid
in addition, weak activity against Gram-positive Staphylococcus aureus and Enterococcus faecalis
(R,Z)-2-(3-((3-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)propanamido)methyl)benzamido)pentanedioic acid
in addition, weak activity against Gram-positive Staphylococcus aureus and Enterococcus faecalis
(R,Z)-2-(3-((4-((2,4-dioxothiazolidin-5-ylidene)methyl)phenylamino)methyl)benzamido)pentanedioic acid
-
(R,Z)-2-(3-((4-((4-oxo-2-thioxothiazolidin-5-ylidene)methyl)phenylamino)methyl)benzamido)pentanedioic acid
-
2,3,4,5-tetrabromo-6-(3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid
inhibitor identified by structure-based virtual screening
2-((4-[(2S)-butan-2-ylamino]-6-(ethylamino)-1,3,5-triazin-2-yl)sulfanyl)-N-(2-chlorophenyl)acetamide
0.5 mM, 50% inhibition. Inhibitory to both MurC and MurD, ECs 6.3.2.8 and 6.3.2.9, respectively
2-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]-5-hydroxybenzoic acid
-
2-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]benzene-1,4-dicarboxylic acid
-
4-([(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl)-6-(naphthalen-2-ylmethyl)-1,3,5-triazin-2-amine
0.5 mM, 33% inhibition. Inhibitory to both MurC and MurD, ECs 6.3.2.8 and 6.3.2.9, respectively
4-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]benzene-1,3-dicarboxylic acid
-
4-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]cyclohexane-1,3-dicarboxylic acid
-
6-([(1,1-dioxidotetrahydrothiophen-3-yl)sulfanyl]methyl)-N-(2-phenylethyl)-1,3,5-triazine-2,4-diamine
0.25 mM, 11% inhibition. Inhibitory to both MurC and MurD, ECs 6.3.2.8 and 6.3.2.9, respectively
6-[(1-methyl-1H-imidazol-2-yl)sulfanyl]-N,N'-diphenyl-1,3,5-triazine-2,4-diamine
0.5 mM, 30% inhibition. Inhibitory to both MurC and MurD, ECs 6.3.2.8 and 6.3.2.9, respectively
N-(3-[[(carboxyacetyl)[4-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl]amino]methyl]benzoyl)-D-glutamic acid
-
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid
competitive versus D-Glu, non-competitive versus ATP and UDP-N-acetylmuramoyl-L-Ala
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid
competitive versus D-Glu, non-competitive versus UDP-N-acetylmuramoyl-L-Ala
N-[2-fluoro-5-[([4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl]amino)methyl]benzoyl]-D-glutamic acid
-
1-phospho-N-acetylmuramoyl-L-Ala-D-Glu
-
-
2,4,6-Trinitrobenzenesulfonic acid
-
-
2-([2-(2-naphthylsulfonyl)hydrazono)methyl]phenyl 2-nitrobenzenesulfonate
-
71% inhibition at 0.05 mM
2-([2-(2-naphthylsulfonyl)hydrazono)methyl]phenyl 3-nitrobenzenesulfonate
-
70% inhibition at 0.05 mM
2-([2-(2-naphthylsulfonyl)hydrazono)methyl]phenyl 4-nitrobenzenesulfonate
-
-
2-([2-(naphthalen-2-ylsulfonyl)hydrazono]methyl)phenyl 2-(benzo[d][1,3]dioxol-5-yl)acetate
-
49% inhibition at 0.10 mM
2-bromo-N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
-
-
3-([[(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]acetyl]amino)-4-methylbenzoic acid
-
-
3-bromo-N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
-
-
4-cyano-N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
-
78% inhibition at 0.05 mM
benzylidene rhodanines
-
possess MurC inhibitory activity in the low micromolar range
-
beta,gamma-methyleneadenosine 5'-triphosphate
-
nonhydrolyzable ATP analogue
CPAHWPHPC
-
i.e. peptide MD-C7C_1, inhibits MurD
CSAWSNKFC
-
i.e. peptide MD-C7C_2, inhibits MurD
D-erythro-3-Methylglutamic acid
-
-
D-erythro-4-Methylglutamic acid
-
-
dihydrouridine 5'-diphosphate-N-acetylmuramoyl-L-Ala-D-Glu
-
-
DL-2-Amino-4-phosphonobutyric acid
-
-
DL-2-aminopimelic acid
-
-
HSSWYIQHFPPL
-
i.e. peptide MD-12, inhibits MurD
N'-((2-[(2-nitrobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
-
-
N'-((2-[(3-nitrobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
-
70% inhibition at 0.05 mM
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)(2-fluorophenyl)methanesulfonohydrazide
-
39% inhibition at 0.10 mM
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)(phenyl)methanesulfonohydrazide
-
58% inhibition at 0.10 mM
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
-
-
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)-2-nitrobenzenesulfonohydrazide
-
86% inhibition at 0.10 mM
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)-3-nitrobenzenesulfonohydrazide
-
74% inhibition at 0.10 mM
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)[4-(trifluoromethyl)phenyl]methanesulfonohydrazide
-
57% inhibition at 0.10 mM
N'-((3-[(3-nitrobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
-
61% inhibition at 0.05 mM
N-(2-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-3,5-dinitrobenzamide
-
-
N-(2-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-4-nitrobenzamide
-
-
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-2-naphthamide
-
19% inhibition at 0.01 mM
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-2-nitrobenzamide
-
-
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-3,5-dinitrobenzamide
-
78% inhibition at 0.10 mM
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-4-nitrobenzamide
-
81% inhibition at 0.05 mM
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
-
56% inhibition at 0.10 mM
N-([(2S)-2-[(2-naphthylsulfonyl)amino]propyl]-sulfonyl)-D-glutamic acid
-
1 mM, 75% residual activity
N-([(2S)-2-[([1,1'-biphenyl]-4-yl-sulfonyl)amino]-propyl]sulfonyl)-D-glutamic acid
-
1 mM, 70% residual activity
N-[((2S)-2-[[(E)-3-(1,3-benzodioxol-5-yl)-2-propenoyl]amino]propyl)sulfonyl]-D-glutamic acid
-
1 mM, 80% residual activity
N-[[(2S)-2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)propyl]sulfonyl]-D-glutamic acid
-
1 mM, 74% residual activity
N-[[(2S)-2-([2-[2-(acetylamino)phenoxy]acetyl]-amino)propyl]sulfonyl]-D-glutamic acid
-
1 mM, 93% residual activity
N-[[2-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-ethyl]sulfonyl]-D-glutamic acid
-
1 mM, 77% residual activity
Phosphinate
-
an analogue of UDP-N-acetylmuramoyl-dipeptide, the N-acetylmuramoyl moiety being replaced by a hexanoyl chain and the peptide bond between L-Ala and D-Glu by a tetrahedral phosphinate bond
potassium phosphate
-
highly dependent on, optimal concentration: 11-16 mM, inhibition above 20 mM, phosphate ion is responsible for inhibition
UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
-
[1-[(6-Uridinediphospho)hexanamido]ethyl](2,4-dicarboxybutyl)phosphinate pentasodium salt
-
good inhibitor, closely resembles the tetrahedral intermediate that is presumed to form the ligation reaction
(2R)-2-[[(7-(4-cyanobenzyloxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
calculation of binding free energies. Main driving force for binding are non-polar van der Waals-interactions
(2R)-2-[[(7-(4-cyanobenzyloxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
NMR and molecular dynamics analysis
additional information
-
synthesis and evaluation of a series of transition-state analog inhibitors
-
additional information
-
design and synthesis of N-benzylidenesulfonohydrazide inhibitors of MurC as antibacterial agents
-
additional information
-
design of peptidomimetic lead compounds with the ultimate objective of small molecule chemotherapeutic development, affinity selection by immobilized MurD with addition of ATP in an attempt to achieve conformationally homogenous population of target enzymes, overview. No inhibition by peptide MD-C7C_3, i.e. CQSSPHMSC
-
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0.006
UDP-N-acetylmuramoyl-L-Ala
-
0.2
1-phospho-N-acetylmuramoyl-L-Ala
-
-
0.01
dihydrouridine 5'-diphosphate-N-acetylmuramoyl-L-Ala
-
-
0.0073
UDP-N-acetylmuramate-L-alanine
-
37°C
0.012 - 0.304
UDP-N-acetylmuramoyl L-alanine
0.0055 - 0.03
UDP-N-acetylmuramoyl-L-Ala
additional information
additional information
ordered kinetic mechanism
-
0.03
ATP
-
pH 9.4, 37°C, E157A mutant protein
0.085
ATP
-
pH 9.4, 37°C, E157K mutant protein
0.114
ATP
-
pH 9.4, 37°C, D35A mutant protein
0.129
ATP
-
pH 9.4, 37°C, K198F mutant protein
0.13
ATP
-
pH 9.4, 37°C, H183A mutant protein
0.135
ATP
-
pH 9.4, 37°C, K198A mutant protein
0.14
ATP
-
pH 9.4, 37°C, N268A mutant protein
0.144
ATP
-
pH 9.4, 37°C, Y194F mutant protein
0.153
ATP
-
pH 9.4, 37°C, H301A mutant protein
0.183
ATP
-
pH 9.4, 37°C, R425A mutant protein
0.328
ATP
-
pH 9.4, 37°C, K115A mutant protein
1.58
ATP
-
pH 9.4, 37°C, N271A mutant protein
2
ATP
-
pH 9.4, 37°C, R302A mutant protein
2.18
ATP
-
pH 9.4, 37°C, D317A mutant protein
0.055
D-Glu
-
-
0.044
D-glutamate
-
pH 9.4, 37°C, D317A mutant protein
0.053
D-glutamate
-
pH 9.4, 37°C, N271A mutant protein
0.053
D-glutamate
-
pH 9.4, 37°C, Y194F mutant protein
0.056
D-glutamate
-
pH 9.4, 37°C, E157K mutant protein
0.074
D-glutamate
-
pH 9.4, 37°C, R302A mutant protein
0.095
D-glutamate
-
pH 9.4, 37°C, D35A mutant protein
0.095
D-glutamate
-
pH 9.4, 37°C, H301A mutant protein
0.128
D-glutamate
-
pH 9.4, 37°C, K115A mutant protein
0.185
D-glutamate
-
pH 9.4, 37°C, K198A mutant protein
0.216
D-glutamate
-
pH 9.4, 37°C, K198F mutant protein
0.264
D-glutamate
-
pH 9.4, 37°C, N268A mutant protein
0.806
D-glutamate
-
pH 9.4, 37°C, H183A mutant protein
5.16
D-glutamate
-
pH 9.4, 37°C, R425A mutant protein
0.012
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, N268A mutant protein
0.016
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, N271A mutant protein
0.04
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, H301A mutant protein
0.046
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, K115A mutant protein
0.053
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, R425A mutant protein
0.057
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, D317A mutant protein
0.059
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, H183A mutant protein
0.065
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, R302A mutant protein
0.084
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, D35A mutant protein
0.111
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, Y194F mutant protein
0.12
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, K198A mutant protein
0.14
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, K198F mutant protein
0.218
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, E157K mutant protein
0.304
UDP-N-acetylmuramoyl L-alanine
-
pH 9.4, 37°C, E157A mutant protein
0.0055
UDP-N-acetylmuramoyl-L-Ala
-
-
0.0075
UDP-N-acetylmuramoyl-L-Ala
-
-
0.03
UDP-N-acetylmuramoyl-L-Ala
-
-
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0.089
(2R)-2-[((3-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication
0.045
(2R)-2-[((4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication
0.192
(2R)-2-[[(7-(2-ethoxy-2-oxoethoxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.132
(2R)-2-[[(7-(3-phenylpropoxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.105
(2R)-2-[[(7-(4-cyanobenzyloxy)naphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.239
(2R)-2-[[(7-benzyloxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.18
(2R)-2-[[(7-butoxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.17
(2R)-2-[[(7-pentoxynaphthalen-2-yl)sulfonyl]amino]pentanedioic acid
Escherichia coli
-
0.01
(2S)-2-[((4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl)carbonyl)amino]pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication
0.005
(R,Z)-2-(3-((1-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)formamido)methyl)benzamido)pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication. Malachite green assay in presence of 0.01% Triton X-100
0.003
(R,Z)-2-(3-((2-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)acetamido)methyl)benzamido)pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication. Malachite green assay in presence of 0.01% Triton X-100
0.007
(R,Z)-2-(3-((3-carboxy-N-(4-((4-oxo-2-thioxothiazolidin-5-ylidene)-methyl)phenyl)propanamido)methyl)benzamido)pentanedioic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication. Malachite green assay in presence of 0.01% Triton X-100
0.085
(R,Z)-2-(3-((4-((2,4-dioxothiazolidin-5-ylidene)methyl)phenylamino)methyl)benzamido)pentanedioic acid
Escherichia coli
pH 8.0, 37°C
0.045
(R,Z)-2-(3-((4-((4-oxo-2-thioxothiazolidin-5-ylidene)methyl)phenylamino)methyl)benzamido)pentanedioic acid
Escherichia coli
pH 8.0, 37°C
0.01
2,3,4,5-tetrabromo-6-(3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid
Escherichia coli
pH 8.0, 37°C
0.046
2-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]-5-hydroxybenzoic acid
Escherichia coli
pH 8.6, 37°C
0.038
2-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]benzene-1,4-dicarboxylic acid
Escherichia coli
pH 8.6, 37°C
0.0084
4-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]benzene-1,3-dicarboxylic acid
Escherichia coli
pH 8.6, 37°C
0.182
4-[([6-[(4-cyano-2-fluorobenzyl)oxy]naphthalen-2-yl]sulfonyl)amino]cyclohexane-1,3-dicarboxylic acid
Escherichia coli
pH 8.6, 37°C
0.015
N-(3-[[(carboxyacetyl)[4-[(Z)-(2,4-dioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl]amino]methyl]benzoyl)-D-glutamic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication. Malachite green assay in presence of 0.01% Triton X-100
0.28
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid
Escherichia coli
-
0.71
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid
Escherichia coli
-
0.252
N-[2-fluoro-5-[([4-[(Z)-(4-oxo-2-thioxo-1,3-thiazolidin-5-ylidene)methyl]phenyl]amino)methyl]benzoyl]-D-glutamic acid
Escherichia coli
pH not specified in the publication, temperature not specified in the publication. Malachite green assay in presence of 0.01% Triton X-100
0.074
2-([2-(2-naphthylsulfonyl)hydrazono)methyl]phenyl 4-nitrobenzenesulfonate
Escherichia coli
-
pH 8.0, 37°C
0.055
2-bromo-N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
Escherichia coli
-
pH 8.0, 37°C
0.105
3-([[(5-amino-1,3,4-thiadiazol-2-yl)sulfanyl]acetyl]amino)-4-methylbenzoic acid
Escherichia coli
-
37°C
0.03
3-bromo-N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)benzamide
Escherichia coli
-
pH 8.0, 37°C
0.177
beta,gamma-methyleneadenosine 5'-triphosphate
Escherichia coli
-
37°C
1.5
CPAHWPHPC
Escherichia coli
-
pH 8.0, 37°C
0.62
CSAWSNKFC
Escherichia coli
-
pH 8.0, 37°C
0.14
HSSWYIQHFPPL
Escherichia coli
-
pH 8.0, 37°C
0.07
N'-((2-[(2-nitrobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
Escherichia coli
-
pH 8.0, 37°C
0.062
N'-((2-[(4-cyanobenzyl)oxy]phenyl)methylidene)-2-naphthalenesulfonohydrazide
Escherichia coli
-
pH 8.0, 37°C
0.045
N-(4-([2-(2-naphthylsulfonyl)hydrazono]methyl)phenyl)-2-nitrobenzamide
Escherichia coli
-
pH 8.0, 37°C
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13
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1998
Escherichia coli, Escherichia coli JM83(pMLD58)
brenda
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Structural and functional characterization of enantiomeric glutamic acid derivatives as potential transition state analogue inhibitors of MurD ligase
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370
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2007
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brenda
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A new approach towards peptidosulfonamides: synthesis of potential inhibitors of bacterial peptidoglycan biosynthesis enzymes MurD and MurE
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62
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2006
Escherichia coli
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brenda
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2008
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Design and synthesis of novel N-benzylidenesulfonohydrazide inhibitors of MurC and MurD as potential antibacterial agents
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13
11-30
2008
Escherichia coli
brenda
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MurD ligase from E. coli: Tetrahedral intermediate formation study by hybrid quantum mechanical/molecular mechanical replica path method
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15
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2008
Escherichia coli
brenda
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Discovery of new inhibitors of the bacterial peptidoglycan biosynthesis enzymes MurD and MurF by structure-based virtual screening
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17
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2009
Escherichia coli (P14900)
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New high-throughput fluorimetric assay for discovering inhibitors of UDP-N-acetylmuramyl-L-alanine: D-glutamate (MurD) ligase
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14
412-418
2009
Escherichia coli
brenda
Simcic, M.; Hodoscek, M.; Humljan, J.; Kristan, K.; Urleb, U.; Kocjan, D.; Grdadolnik, S.G.
NMR and molecular dynamics study of the binding mode of naphthalene-N-sulfonyl-D-glutamic acid derivatives: novel MurD ligase inhibitors
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52
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2009
Escherichia coli (P14900)
brenda
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Binding free energy calculations of N-sulphonyl-glutamic acid inhibitors of MurD ligase
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15
983-996
2009
Escherichia coli (P14900)
brenda
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Second-generation sulfonamide inhibitors of d-glutamic acid-adding enzyme: Activity optimisation with conformationally rigid analogues of D-glutamic acid
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46
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2011
Escherichia coli (P14900)
brenda
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Novel 2-thioxothiazolidin-4-one inhibitors of bacterial MurD ligase targeting D-Glu- and diphosphate-binding sites
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46
3964-3975
2011
Escherichia coli (P14900), Escherichia coli
brenda
Zidar, N.; Tomasic, T.; Sink, R.; Rupnik, V.; Kovac, A.; Turk, S.; Patin, D.; Blanot, D.; Contreras Martel, C.; Dessen, A.; Mueller Premru, M.; Zega, A.; Gobec, S.; Peterlin Masic, L.; Kikelj, D.
Discovery of novel 5-benzylidenerhodanine and 5-benzylidenethiazolidine-2,4-dione inhibitors of MurD ligase
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53
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2010
Escherichia coli (P14900), Escherichia coli
brenda
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54
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2011
Escherichia coli (P14900), Escherichia coli
brenda
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18
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2012
Escherichia coli (P14900)
brenda
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Crystallographic study of peptidoglycan biosynthesis enzyme MurD: domain movement revisited
PLoS ONE
11
e0152075
2016
Escherichia coli (P14900)
brenda