Information on EC 6.3.2.45 - UDP-N-acetylmuramate-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
6.3.2.45
-
RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylmuramate-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
anhydromuropeptides recycling
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase
The enzyme catalyses the reincorporation into peptidoglycan of the tripeptide L-alanyl-gamma-D-glutamyl-2,6-meso-diaminoheptanedioate released during the maturation and constant remodeling of this bacterial cell wall polymer that occur during cell growth and division. The enzyme can also use the tetrapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine or the pentapeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine in vivo and in vitro. Requires Mg2+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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the recycling enzyme allows reincorporation into peptidoglycan (murein) of the tripeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate released during the maturation and constant remodeling of this bacterial cell wall polymer that occur during cell growth and division. The enzyme adds this peptide to UDP-N-acetylmuramic acid, thereby providing an economical additional source of UDP-MurNAc-tripeptide available for de novo peptidoglycan biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-alpha-D-acetylmuramate + L-Ala-D-Glu
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-alpha-D-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-gamma-D-Glu-L-Lys
show the reaction diagram
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-
-
-
?
ATP + UDP-N-alpha-D-acetylmuramate + L-Ala-gamma-D-Glu-L-Lys-D-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-alpha-D-acetylmuramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
ATP + UDP-N-alpha-D-acetylmuramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine
show the reaction diagram
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-
-
-
?
ATP + UDP-N-alpha-D-acetylmuramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-alpha-D-acetylmuramate + L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
show the reaction diagram
-
the recycling enzyme allows reincorporation into peptidoglycan (murein) of the tripeptide L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate released during the maturation and constant remodeling of this bacterial cell wall polymer that occur during cell growth and division. The enzyme adds this peptide to UDP-N-acetylmuramate, thereby providing an economical additional source of UDP-MurNAc-tripeptide available for de novo peptidoglycan biosynthesis
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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absolute requirement for a divalent cation. The optimal concentration for Mg2+ is 15 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
ATP
-
pH 8.4, 37°C
19
L-Ala-D-Glu
-
pH 8.4, 37°C
4.1
L-Ala-gamma-D-Glu-L-Lys
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pH 8.4, 37°C
3.9
L-Ala-gamma-D-Glu-L-Lys-D-Ala
-
pH 8.4, 37°C
0.1
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
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pH 8.4, 37°C
0.1
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine
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pH 8.4, 37°C
0.051
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine
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pH 8.4, 37°C
0.25
UDP-N-alpha-D-acetylmuramate
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pH 8.4, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.83
ATP
Escherichia coli
-
pH 8.4, 37°C
0.01 - 1
L-Ala-D-Glu
Escherichia coli
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pH 8.4, 37°C
0.27
L-Ala-gamma-D-Glu-L-Lys
Escherichia coli
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pH 8.4, 37°C
0.32
L-Ala-gamma-D-Glu-L-Lys-D-Ala
Escherichia coli
-
pH 8.4, 37°C
4.83
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
Escherichia coli
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pH 8.4, 37°C
3.17
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine
Escherichia coli
-
pH 8.4, 37°C
2.83
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine
Escherichia coli
-
pH 8.4, 37°C
4.83
UDP-N-alpha-D-acetylmuramate
Escherichia coli
-
pH 8.4, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25
ATP
Escherichia coli
-
pH 8.4, 37°C
4
0.00058
L-Ala-D-Glu
Escherichia coli
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pH 8.4, 37°C
194908
0.065
L-Ala-gamma-D-Glu-L-Lys
Escherichia coli
-
pH 8.4, 37°C
170987
0.082
L-Ala-gamma-D-Glu-L-Lys-D-Ala
Escherichia coli
-
pH 8.4, 37°C
133488
48
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate
Escherichia coli
-
pH 8.4, 37°C
194905
32
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanine
Escherichia coli
-
pH 8.4, 37°C
194906
55
L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioyl-D-alanyl-D-alanine
Escherichia coli
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pH 8.4, 37°C
194907
20
UDP-N-alpha-D-acetylmuramate
Escherichia coli
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pH 8.4, 37°C
194904
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
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pH 8.4, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6 - 9.2
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pH 7.6: about 75% of maximal activity, pH 9.2: about 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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x * 50000, SDS-PAGE
50940
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x * 50940, calculated from sequence
50961
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x * 50961, MALDI-TOF mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 50000, SDS-PAGE; x * 50940, calculated from sequence; x * 50961, MALDI-TOF mass spectrometry
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed under control of the trc promoter
overproduction as a His6-tagged protein
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Show AA Sequence (3457 entries)
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