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Information on EC 6.3.2.42 - N-acetylaspartylglutamylglutamate synthase and Organism(s) Mus musculus and UniProt Accession Q6PFX8

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EC Tree
IUBMB Comments
The enzyme, found in mammals, also has the activity of EC 6.3.2.41, N-acetylaspartylglutamate synthase.
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This record set is specific for:
Mus musculus
UNIPROT: Q6PFX8
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
N-acetylaspartylglutamate synthetase II, N-acetylaspartylglutamylglutamate synthetase, NAAG synthetase II, NAAG(2) synthase, NAAGS-II, RIMKLA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N-acetylaspartylglutamylglutamate synthetase
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NAAG synthetase II
-
NAAG(2) synthase
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RIMKLA
gene name, ambiguous
N-acetylaspartylglutamate synthetase II
-
-
NAAGS-II
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
N-acetyl-L-aspartate:L-glutamate ligase (ADP, N-acetyl-L-aspartyl-L-glutamyl-L-glutamate-forming)
The enzyme, found in mammals, also has the activity of EC 6.3.2.41, N-acetylaspartylglutamate synthase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
show the reaction diagram
ATP + N-acetyl-L-aspartate + L-glutamate
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
?
ATP + N-acetyl-L-aspartate + L-glutamate
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression levels in the brain stem and spinal cord, almost undetectable in the neocortex
Manually annotated by BRENDA team
strong expression is restricted to deep cerebellar nuclei
Manually annotated by BRENDA team
expression is found in the ventral and dorsal horns of gray matter in laminae III to X, but is low or absent in laminae I and II
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RIMKA_MOUSE
380
0
41504
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
x * 42000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42000, SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells. Because hexahistidine-tagged Escherichia coli-expressed enzyme using bacterial expressed protein
expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lodder-Gadaczek, J.; Becker, I.; Gieselmann, V.; Wang-Eckhardt, L.; Eckhardt, M.
N-Acetylaspartylglutamate synthetase II synthesizes N-acetylaspartylglutamylglutamate
J. Biol. Chem.
286
16693-16706
2011
Mus musculus, Mus musculus (Q6PFX8)
Manually annotated by BRENDA team