Information on EC 6.3.2.37 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-D-lysine ligase

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The expected taxonomic range for this enzyme is: Thermotoga maritima

EC NUMBER
COMMENTARY hide
6.3.2.37
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RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-D-lysine ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + D-lysine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-Nepsilon-D-lysine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate:D-lysine gamma-ligase (ADP-forming)
Involved in the synthesis of cell-wall peptidoglycan. The D-lysine is attached to the peptide chain at the N6 position. The enzyme from Thermotoga maritima also performs the reaction of EC 6.3.2.7, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetylmuramoyl-L-Ala-D-Glu + D-Lys + ATP
UDP-N-acetylmuramoyl-L-Ala-D-Glu-D-Lys + ADP + phosphate
show the reaction diagram
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D-lysine is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 25% efficiency relative to L-lysine
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?
UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys + ATP
UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + ADP + phosphate
show the reaction diagram
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?
UDP-N-acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid + ATP
UDP-N-acetylmuramoyl-L-Ala-D-Glu-meso-diaminopimelic acid + ADP + phosphate
show the reaction diagram
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meso-diaminopimelic acid is added to UDP-N-acetylmuramoyl-L-Ala-D-Glu with 10% efficiency relative to L-lysine
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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the optimum Mg2+ concentration for L-lysine adding activity is 15 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UDP-N-acetylmuramoyl-L-Ala-D-Glu
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strong inhibition by excess UDP-N-acetylmuramoyl-L-Ala-D-Glu is observed
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.4
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for L-lysine adding activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68
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temperature optimum for L-lysine adding activity. At 37°C, the activity is about 25% of the optimal value
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
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SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography, gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as C-terminal His6-tagged enzyme in Escherichia coli DH5alpha cells
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