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Information on EC 6.3.2.25 - tubulin-tyrosine ligase and Organism(s) Rattus norvegicus and UniProt Accession Q9QXJ0

for references in articles please use BRENDA:EC6.3.2.25
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EC Tree
IUBMB Comments
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q9QXJ0
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ttl, tubulin tyrosine ligase, tubulin-tyrosine ligase, tubulin:tyrosine ligase, tyrosyltubulin ligase, ttlase, tubulin-tyrosine-ligase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tubulin tyrosine ligase
-
Synthetase, tubulin-tyrosine
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-
-
-
TTL
-
-
-
-
TTLase
-
-
-
-
Tubulin:tyrosine ligase
-
-
-
-
Tyrosyltubulin ligase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formation of peptide bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-tubulin:L-tyrosine ligase (ADP-forming)
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
CAS REGISTRY NUMBER
COMMENTARY hide
60321-03-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + detyrosinated alpha-tubulin + L-tyrosine
alpha-tubulin + ADP + phosphate
show the reaction diagram
-
-
?
ATP + detyrosinated alpha-tubulin + L-Tyr
alpha-tubulin + ADP + phosphate
show the reaction diagram
-
r
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + detyrosinated alpha-tubulin + L-tyrosine
alpha-tubulin + ADP + phosphate
show the reaction diagram
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TTL_RAT
377
0
43118
Swiss-Prot
other Location (Reliability: 3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transient transfection of a TTL cDNA portion that encodes the beta-tubulin binding domain but lacks the catalytic domain into CHO-K1 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Argarana, C.E.; Barra, H.S.; Caputto, R.
Release of [14C]tyrosine from tubulinyl-[14C]tyrosine by brain extract separation of a carboxypeptidase from tubulin-tyrosine ligase
Mol. Cell. Biochem.
19
17-21
1978
Rattus norvegicus
Manually annotated by BRENDA team
MacRae, T.H.
Tubulin post-translational modifications
Eur. J. Biochem.
244
265-278
1997
Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Mas, C.R.; Arregui, C.O.; Filiberti, A.; Argarana, C.E.; Barra, H.S.
Cloning of rat olfactory bulb tubulin tyrosine ligase cDNA: a dominant negative mutant and an antisense cDNA increase the proliferation rate of cells in culture
Neurochem. Res.
27
1453-1458
2002
Rattus norvegicus (Q9QXJ0)
Manually annotated by BRENDA team