Information on EC 6.3.2.24 - tyrosine-arginine ligase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
6.3.2.24
-
RECOMMENDED NAME
GeneOntology No.
tyrosine-arginine ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-tyrosine + L-arginine = AMP + diphosphate + L-tyrosyl-L-arginine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
peptide synthase reaction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-tyrosine:L-arginine ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
116036-78-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
-
-
37.8% of activity compared to ATP
-
AMP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
-
-
1,6% of activity compared to ATP
?
ATP + L-arginine + leucine
? + AMP
show the reaction diagram
-
-
3.5% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + lysine
? + AMP
show the reaction diagram
-
-
10.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + phenylalanine
? + AMP
show the reaction diagram
-
-
10% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-arginine + tryptophan
? + AMP
show the reaction diagram
-
-
11.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + glutamate
? + AMP
show the reaction diagram
-
-
26.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + glycine
? + AMP
show the reaction diagram
-
-
1.1% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + histidine
? + AMP
show the reaction diagram
-
-
4.0% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + AMP + diphosphate
show the reaction diagram
ATP + L-tyrosine + leucine
? + AMP
show the reaction diagram
-
-
31.4% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
ATP + L-tyrosine + lysine
? + AMP
show the reaction diagram
-
-
14.3% the rate of AMP formed by ATP/L-tyrosine/L-arginine
?
GTP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + ?
show the reaction diagram
-
-
20.2% of activity compared to ATP
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-tyrosine + L-arginine
L-tyrosyl-L-arginine + AMP + diphosphate
show the reaction diagram
-
involved in biosynthesis of kyotorphin in rat brain
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
102,7% of activity compared to Mg2+, may substitute for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-methyl-L-ornithine
Co2+
-
0.4-4 mM: strong
Cu2+
-
0.04-4 mM: strong
D-arginine
-
-
diphosphate
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10 mM: 97.6% inhibition
K+
-
50-100 mM: strong
NG-nitro-L-arginine methyl ester
NH4+
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10-100 mM: strong
phosphate
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10 mM: 4.2% inhibition
additional information
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not inhibitory: NG-nitro-L-arginine, N-iminoethyl-L-ornithine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 0.294
ATP
0.926 - 7.83
L-arginine
0.0256 - 0.07
L-tyrosine
0.442
MgCl2
-
37C, pH 8.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000016
-
37C, pH 8.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
-
broad
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10
-
28% of maximal activity at pH 8, 85% of maximal activity at pH 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
highest activity in synaptosolic fraction, minor activity membrane-bound or in solubilized fraction
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
200000 - 300000
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gel filtration, both enzyme from adrenal gland as well as from spinal cord
240000 - 245000
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE