Information on EC 6.3.2.19 - Ubiquitin-protein ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
6.3.2.19
-
RECOMMENDED NAME
GeneOntology No.
Ubiquitin-protein ligase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
both an enzyme-bound COOH-terminal ubiquitin thiolester and a COOH-terminal ubiquitin adenylate are formed
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
thiol ester intermediate
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
substrate binding and product release are both strictly ordered, with ATP the leading substrate with respect to ubiquitin and diphosphate the leading product with respect to AMP
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
the ubiquitin-activating enzyme E1 catalyzes the formation of ubiquitin adenylate and the transfer of activated ubiquitin to a thiol site on the same enzyme. Following activation, ubiquitin is transferred from the thiol site of E1 to the thiol site of one of several ubiquitin carrier proteins, known as E2s
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
a covalent possibly thiol ester intermediate, is formed between the activating enzyme and ubiquitin
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
reaction sequence: 1. initial formation of tightly enzyme-bound ubiquitin adenylate with diphosphate formation from ATP, 2. conversion of this intermediate to form AMP and a covalent enzyme-ubiquitin thiolester, 3. activation of a second molecule of ubiquitin to give a ternary complex of one equivalent each of ubiquitin thiolester and tightly bound ubiquitin adenylate per subunit of enzyme
-
ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
the COOH-terminal Gly of ubiquitin is activated by the enzyme
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carboxamide formation
-
-
-
-
carboxylic acid amide formation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
Ubiquitin:protein-lysine N-ligase (AMP-forming)
Ubiquitin is coupled to protein by a peptide bond between the C-terminal glycine of ubiquitin and epsilon-amino groups of lysine residues in the protein. An intermediate in the reaction contains one ubiquitin residue bound as a thioester to the enzyme, and a residue of ubiquitin adenylate non-covalently bound to the enzyme.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
APF-1-protein amide synthetase
-
-
-
-
Bendless protein
-
-
-
-
Bendless-like ubiquitin conjugating enzyme
-
-
-
-
Cell division control protein 34
-
-
-
-
E2-17 kDa
-
-
-
-
E2-20K
-
-
-
-
E2-CDC34
-
-
-
-
E2-EPF5
-
-
-
-
E2-F1
-
-
-
-
E217K
-
-
-
-
Effete protein
-
-
-
-
HHR6A
-
-
-
-
HHR6B
-
-
-
-
HR6A
-
-
-
-
HR6B
-
-
-
-
L-UBC
-
-
-
-
Nedd8-conjugating enzyme Ubc12
-
-
-
-
P18
-
-
-
-
PM42
-
-
-
-
RAD6 homolog
-
-
-
-
Retinoic acid induced gene B protein
-
-
-
-
SUMO-1-protein ligase
-
-
-
-
Synthetase, ubiquitin-protein
-
-
-
-
TAYO29
-
-
-
-
Ubc13
-
-
-
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UBC7
-
-
-
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UBCAT4A
-
-
-
-
UBCAT4B
-
-
-
-
UbcH10
-
-
-
-
UBCH2
-
-
-
-
UbcH6
-
-
-
-
UbcM4
-
-
-
-
Ubiquitin carrier protein
-
-
-
-
Ubiquitin carrier protein 10/12
-
-
-
-
Ubiquitin carrier protein 11
-
-
-
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Ubiquitin carrier protein G1
-
-
-
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Ubiquitin carrier protein G2
-
-
-
-
Ubiquitin carrier protein HUS5
-
-
-
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Ubiquitin-activating enzyme
-
-
-
-
Ubiquitin-conjugating enzyme 15
-
-
-
-
Ubiquitin-conjugating enzyme UbcE2A
-
-
-
-
Ubiquitin-protein ligase
-
-
-
-
Ubiquitin-protein ligase 10/12
-
-
-
-
Ubiquitin-protein ligase 11
-
-
-
-
Ubiquitin-protein ligase G1
-
-
-
-
Ubiquitin-protein ligase G2
-
-
-
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Ubiquitin-protein ligase HUS5
-
-
-
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Ubiquitin-protein synthetase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
74812-49-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Col-0
-
-
Manually annotated by BRENDA team
ecotype Columbia
UniProt
Manually annotated by BRENDA team
strain AR58, wild type and mutant enzyme
-
-
Manually annotated by BRENDA team
Escherichia coli AR58
strain AR58, wild type and mutant enzyme
-
-
Manually annotated by BRENDA team
E6-AP
UniProt
Manually annotated by BRENDA team
EDD
UniProt
Manually annotated by BRENDA team
four MGRN isozymes, mahoganoid
UniProt
Manually annotated by BRENDA team
Huwe1
UniProt
Manually annotated by BRENDA team
isozyme Pirh2A; isozyme Pirh2A; isozyme Pirh2B; isozyme Pirh2B; isozyme Pirh2C; isozyme Pirh2C
SwissProt
Manually annotated by BRENDA team
Itch
UniProt
Manually annotated by BRENDA team
Nedd4
UniProt
Manually annotated by BRENDA team
WWP1
UniProt
Manually annotated by BRENDA team
strain Paris (Lpp2887)
Uniprot
Manually annotated by BRENDA team
Legionella pneumophila Paris (Lpp2887)
strain Paris (Lpp2887)
Uniprot
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
mahoganoid
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
Triticum vulgare
-
-
Manually annotated by BRENDA team
wheat, wild type and mutant enzyme
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx is involved in skeletal muscle protein breakdown in rats with thermal injury
malfunction
-
Huwe1 balances proliferation and neurogenesis in the developing brain which is subverted in malignant brain tumors
malfunction
-
mahogunin ring finger-1, MGRN1, is a RING domain-containing ubiquitin ligase mutated in mahoganoid, a mouse mutation causing coat color darkening, congenital heart defects, high embryonic lethality, and spongiform neurodegeneration. MGRN1 isoforms decrease MC1R and MC4R signaling to cAMP, without effect on beta2-adrenergic receptor and independently on receptor plasma membrane expression, ubiquitylation, internalization, or stability and occurred upstream of Galphas binding to/activation of adenylyl cyclase. Overexpression of Galpha5 abolishes the inhibitory effect of MGRN1
malfunction
-
ubiquitin ligases are associated with several diseases, e.g. cancer, neurodegeneration, viral infection, and metabolinc and immune diesases
malfunction
-
downregulation of IBRDC2 induces increased cellular levels and accumulation of the active form of Bax
malfunction
-
mice with a MYCBP2-deficiency in peripheral sensory neurons show prolonged thermal hyperalgesia. Loss of MYCBP2 constitutively activates p38 MAPK and increases expression of several proteins involved in receptor trafficking. Loss of MYCBP2 inhibits internalization of transient receptor potential vanilloid receptor 1 and prevents desensitization of capsaicin-induced calcium increases
malfunction
-
MURF3 knockdown in cardiomyocytes by an isogene-specific siRNA leads to upregulation of MURF2 expression. Knockdown of both MURF2 and MURF3 severely disrupts the formation of ordered Z- and M-bands, likely by perturbed tubulin dynamics. It is suggested that ubiquitin-mediated protein turnover and MURF2 in particular play an unrecognised role in the earliest steps of heart muscle differentiation, and that partial complementation of MURF2 deficiency is afforded by MURF3
malfunction
-
selective genetic inactivation of Cbl-b E3 ligase activity (C373AKI/KI knock-in) phenocopies the T cell responses observed when total Cbl-b is ablated, resulting in T cell hyperactivation, spontaneous autoimmunity, and impaired induction of T cell anergy in vivo. Mice carrying a Cbl-b E3 ligase-defective mutation spontaneously reject tumor cells that express human papilloma virus Ags
malfunction
-
silencing of Mule stabilizes Miz1, thereby suppressing TNFalpha-induced JNK activation and cell death
malfunction
-
siRNA knockdown of MURF2 in neonatal rat cardiomyocytes disrupts posttranslational microtubule modification and myofibril assembly, and is only partly compensated by upregulation of MURF3 but not MURF1. It is suggested that ubiquitin-mediated protein turnover and MURF2 in particular play an unrecognised role in the earliest steps of heart muscle differentiation, and that partial complementation of MURF2 deficiency is afforded by MURF3
malfunction
-
the knockdown of Fbxo45 in primary cultured hippocampal neurons results in a greater frequency of miniature excitatory postsynaptic currents
metabolism
-
the enzyme is essential in the intercellular Notch signaling pathway, overview
metabolism
-
the enzyme is involved in the ubiquitin-proteasome pathway, UPS, where it mediates the ubiquitin transfer from E2, the ubiquitin-conjugating enzyme to the lysine residue(s) of the substrate
metabolism
-
the ubiquitin ligase Huwe1 operates upstream of the N-Myc-DLL3-Notch pathway to control neural stem cell activity and promote neurogenesis, it is part of the Huwe1-N-myc pathway
physiological function
-
E3 ubiquitin ligases target specific molecules for proteolytic destruction and are key regulators of immune functions. The HECT-type E3 ligase AIP2 positively regulates T-cell activation, and AIP2 regulates activation-induced T-cell death by suppressing EGR2-mediated FasL expression via the ubiquitin pathway
physiological function
-
E6-AP, a HECT domain family ubiquitin ligase implicated in Angelman syndrome, interacts with the substrate binding domain of Hsp70/Hsc70 chaperones and promotes the degradation of chaperone bound substrates
physiological function
O60291
MGRN-1 is one of two accessory proteins for MC signaling, it inhibits functional coupling of MC1R and MC4R to the cAMP pathway, mechanism, overview. All MGRN1 isozymes decrease agonist-induced cAMP production by as much as 50%
physiological function
-
MGRN-1 is one of two accessory proteins for MC signaling, it inhibits functional coupling of MC1R and MC4R to the cAMP pathway, overview
physiological function
P35131
RMA E3 Ub ligases may play a role in the growth and development of Arabidopsis thaliana
physiological function
-
subunit Fbw7 is considered to be a potent tumor suppressor
physiological function
-
the CUL4A–DDB1–ROC1–beta-TRCP complex is a major E3 ligase that regulates REDD1 stability
physiological function
-
the ubiquitin ligase Huwe1 operates upstream of the N-Myc-DLL3-Notch pathway to control neural stem cell activity and promote neurogenesis. It acts as tumor suppressor gene
physiological function
-
Fbxo45 regulates neurotransmission at mature neurons. Fbxo45 induces the degradation of the synaptic vesicle-priming factor Munc13-1. Fbxo45 plays an important role in the regulation of neurotransmission by modulating Munc13-1 at the synapse
physiological function
-
IBRDC2 is a regulatory factor for Bax and protects cells from unprompted Bax activation and cell death
physiological function
-
MAPK kinase 4/SEK1 is negatively regulated through a feedback loop involving the E3 ubiquitin ligase Itch. Itch controls MAPK kinase 4 stability
physiological function
-
MYCBP2 regulates internalization of transient receptor potential vanilloid receptor 1 in peripheral sensory neurons as well as duration of thermal hyperalgesia through p38 MAPK. MYCBP2 negatively controls neuronal growth
physiological function
Q9HCE7
Smurf1 plays a critical role in embryogenesis and adult bone homeostasis
physiological function
-
the BRCA1-BARD1 RING complex has an E3 ubiquitin ligase activity that plays an essential role in response to DNA damage
physiological function
-
the catalytic function of the E3 ligase Cbl-b is essential for negative regulation of T cells in vivo. Cbl-b E3 ligase activity is required for in vivo T cell tolerance
physiological function
-
the E3 ubiquitin ligase Itch regulates sorting nexin 9 through an unconventional substrate recognition domain
physiological function
-
the selective enrichment of Epe1 at boundaries requires its regulation by the conserved Cul4-Ddb1Cdt2 ubiquitin ligase, which directly recognizes Epe1 and promotes its polyubiquitylation and degradation. Epe1 is the sole target of the Cul4-Ddb1Cdt2 complex whose destruction is necessary for the preservation of heterochromatin
physiological function
-
the ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C-terminal portion of Bid
physiological function
-
UbcM2 regulates the stability and transcriptional activity of the antioxidant transcription factor Nrf2 (nuclear factor E2-related factor 2). UbcM2 is involved in the restoration of redox homeostasis following oxidative stress
physiological function
-
Ube2 participates in fertilization or spermatogenesis/spermiogenesis
physiological function
-
ubiquitin ligase Mule is required for TNFalpha-induced JNK activation
physiological function
Mus musculus C57BL/6
-
E3 ubiquitin ligases target specific molecules for proteolytic destruction and are key regulators of immune functions. The HECT-type E3 ligase AIP2 positively regulates T-cell activation, and AIP2 regulates activation-induced T-cell death by suppressing EGR2-mediated FasL expression via the ubiquitin pathway
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + K0-ubiquitin + UbcH7 protein lysine
AMP + diphosphate + UbcH7 protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + ABRA1
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + AIB1
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + AKIN10
?
show the reaction diagram
Q9M0V3
PRL1 is the substrate receptor of a CUL4-ROC1-DDB1-PRL1 E3 ligase involved in the degradation of AKIN10
-
-
?
ATP + ubiquitin + alpha-synphilin-1
AMP + diphosphate + alpha-synphilin-1 N-ubiquityllysine
show the reaction diagram
-
parkin ubiquitinates proteins contained within cytosolic Lewy-body-like inclusions that contain both synphilin and alpha-synuclein. Familial-linked mutations in parkin impair the ubiquitination of proteins within these cytosolic inclusions
-
-
?
ATP + ubiquitin + alpha-synuclein
AMP + diphosphate + alpha-synuclein N-ubiquityllysine
show the reaction diagram
-
22000 Da glycosylated form of alpha-synuclein, the reaction is altered in Parkinson‘s disease, the loss of parkin function causes pathological accumulation of alpha-synuclein
-
-
?
ATP + ubiquitin + alpha-synuclein
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + APOBEC3G lysine
AMP + diphosphate + APOBEC3G N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + APOBEC3G lysine
AMP + diphosphate + APOBEC3G N-ubiquityllysine
show the reaction diagram
-
substrate of the E3 ligase Cullin5-ElonginB+C
-
-
?
ATP + ubiquitin + apolipoprotein B-editing catalytic subunit 3 D/E lysine
AMP + diphosphate + apolipoprotein B-editing catalytic subunit 3 D/E N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + apolipoprotein B-editing catalytic subunit 3 F lysine
AMP + diphosphate + apolipoprotein B-editing catalytic subunit 3 F N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + apolipoprotein B-editing catalytic subunit 3 G lysine
AMP + diphosphate + apolipoprotein B-editing catalytic subunit 3 G N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + APP-BP1
?
show the reaction diagram
Q14669
TRIP12 functions as an E3 enzyme of APP-BP1 and additionally requires an E4 activity for polyubiquitination of APP-BP1, TRIP12 specifically interacts with the APP-BP1 monomer but not with the APP-BP1/Uba3 heterodimer
-
-
?
ATP + ubiquitin + aurora A
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + aurora B
?
show the reaction diagram
-
substrate of APC/C and Cul3
-
-
?
ATP + ubiquitin + B7.2
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + BACH1
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + Bag-1 lysine
AMP + diphosphate + Bag-1 N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + Bak
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + Bcl2/adenovirus E1B 19-kD-interacting protein 3
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + beta-amyloid precursor protein-binding, family B, member 1-interacting protein
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + beta-catenin lysine
AMP + diphosphate + beta-catenin N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + beta-catenin lysine
AMP + diphosphate + beta-catenin N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + Blk
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + BMP-RI/II lysine
AMP + diphosphate + BMP-RI/II ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + c-Jun
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + c-Jun
AMP + diphosphate + c-Jun N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + c-Jun lysine
AMP + diphosphate + c-Jun N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + c-myb lysine
AMP + diphosphate + c-myb N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + c-myb lysine
AMP + diphosphate + c-myb N-ubiquityllysine
show the reaction diagram
-
substrate of SCFFbw7
-
-
?
ATP + ubiquitin + c-Myc
?
show the reaction diagram
Q8VBV4
-
-
-
?
ATP + ubiquitin + c-Myc
?
show the reaction diagram
-
substrate of isoforms Fbw7alpha and Fbw7gamma
-
-
?
ATP + ubiquitin + c-Myc lysine
AMP + diphosphate + c-Myc N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + c-Myc lysine
AMP + diphosphate + c-Myc N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + c-Myc lysine
AMP + diphosphate + c-Myc N-ubiquityllysine
show the reaction diagram
-
substrate of SCFFbw7
-
-
?
ATP + ubiquitin + c-Ski lysine
AMP + diphosphate + c-Ski N-ubiquityllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Arkadis enhances TGF-beta signaling by inducing ubiquitin-dependent degradation of c-Ski
-
-
?
ATP + ubiquitin + caspase 3 lysine
AMP + diphosphate + caspase 3 N-ubiquityllysine
show the reaction diagram
-
XIAP polyubiquitinates caspase-3 when it is overexpressed
-
-
?
ATP + ubiquitin + CD166
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + CD1d
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + CD3-delta lysine
AMP + diphosphate + CD3-delta N-ubiquityllysine
show the reaction diagram
-
gp78 targets CD3-delta for proteasomal degradation
-
?
ATP + ubiquitin + CD31
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + Cdc2-like kinase 1
?
show the reaction diagram
Legionella pneumophila, Legionella pneumophila Paris (Lpp2887)
Q5ZRQ0
polyubiquitination, Cdc2-like kinase 1 is the target host molecule which Legionella modulates during infection
-
-
?
ATP + ubiquitin + Cdc20
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + Cdc25A
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Cdc25A
?
show the reaction diagram
-
substrate of SCF and APC/C
-
-
?
ATP + ubiquitin + Cdc25p lysine
AMP + diphosphate + Cdc25p N-ubiquityllysine
show the reaction diagram
Q92462, Q94TG2
-
-
-
?
ATP + ubiquitin + Cdc28 protein kinase regulatory subunit 1B
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Cdc4 lysine
AMP + diphosphate + Cdc4 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + CDC6
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + Cdc6 lysine
AMP + diphosphate + Cdc6 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + CDCrel-1
AMP + diphosphate + CDCrel-1 N-ubiquityllysine
show the reaction diagram
Q9Y4X5
-
-
-
?
ATP + ubiquitin + CDT1
?
show the reaction diagram
-
substrate of SCF, Cul4A, and APC/C
-
-
?
ATP + ubiquitin + Cdt1 lysine
AMP + diphosphate + Cdt1 N-ubiquitinyllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Cdt1 lysine
AMP + diphosphate + Cdt1 N-ubiquitinyllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Cep-1 lysine
AMP + diphosphate + Cep-1 N-ubiquityllysine
show the reaction diagram
Q18223
endogenous CEP-1 protein phosphorylation levels are regulated by FSN-1
-
-
?
ATP + ubiquitin + CHIP lysine
AMP + diphosphate + CHIP N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + Cks1
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + CtIP
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + CTL
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + CUP9 lysine
AMP + diphosphate + CUP9 N-ubiquityllysine
show the reaction diagram
P19812
-
-
-
?
ATP + ubiquitin + CUP9 lysine
AMP + diphosphate + CUP9 N-ubiquityllysine
show the reaction diagram
Saccharomyces cerevisiae ATCC 204508
-
-
-
-
?
ATP + ubiquitin + CYC4 lysine
AMP + diphosphate + CYC4 N-ubiquityllysine
show the reaction diagram
-
auto ubiquitination
-
?
ATP + ubiquitin + cyclin A
?
show the reaction diagram
-
UbcH10 is specifically required for ubiquitination of cyclin A by APC/C during mitosis
-
-
?
ATP + ubiquitin + cyclin B1
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + cyclin D1
?
show the reaction diagram
-
substrate of SCF
-
-
?
ATP + ubiquitin + cyclin E
AMP + diphosphate + cyclin E N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + cyclin E
?
show the reaction diagram
Q8VBV4
-
-
-
?
ATP + ubiquitin + cyclin E
?
show the reaction diagram
-
substrate of isoform Fbw7alpha, Fbw7aqlpha does not directly ubiquitinylate cyclin E but instead promotes cyclin E proline 382 isomerization by Pin1, after which cyclin E is ubiquitinylated by Fbw7gamma
-
-
?
ATP + ubiquitin + cyclin E
?
show the reaction diagram
-
substrate of SCF, Cul3, and Cul4B
-
-
?
ATP + ubiquitin + cyclin E lysine
AMP + diphosphate + cyclin E N-ubiquityllysine
show the reaction diagram
-
substrate of SCFFbw7
-
-
?
ATP + ubiquitin + cyclin-dependent kinase inhibitor-1 lysine
AMP + diphosphate + cyclin-dependent kinase inhibitor-1 N-ubiquitinyllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + DCC lysine
AMP + diphosphate + DCC N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + DELTANp63alpha lysine
AMP + diphosphate + DELTANp63alpha N-ubiquityllysine
show the reaction diagram
-
UFD2a stabilizes DELTANp63alpha, and ubiquitylation is attenuated by UFD2a both in the presence and absence of cisplatin, UFD2a may play an important role in the regulation of cisplatin-mediated cell death by p63
-
-
?
ATP + ubiquitin + EGR2 lysine
AMP + diphosphate + EGR2 N-ubiquityllysine
show the reaction diagram
-
AIP2 promotes EGR2 ubiquitination and degradation. AIP2 interacts with and promotes ubiquitin-mediated degradation of EGR2, a zinc finger transcription factor that regulates Fas ligand expression during activation-induced T-cell death, substrate interaction via its PPXY motifs
-
-
?
ATP + ubiquitin + EGR2 lysine
AMP + diphosphate + EGR2 N-ubiquityllysine
show the reaction diagram
Mus musculus C57BL/6
-
AIP2 promotes EGR2 ubiquitination and degradation. AIP2 interacts with and promotes ubiquitin-mediated degradation of EGR2, a zinc finger transcription factor that regulates Fas ligand expression during activation-induced T-cell death, substrate interaction via its PPXY motifs
-
-
?
ATP + ubiquitin + Emi1
?
show the reaction diagram
-
substrate of SCF
-
-
?
ATP + ubiquitin + ENaC-beta protein lysine
AMP + diphosphate + ENaC-beta protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Epe1 lysine
AMP + diphosphate + Epe1 N-ubiquityllysine
show the reaction diagram
-
Epe1 is the sole target of the Cul4-Ddb1Cdt2 complex
-
-
?
ATP + ubiquitin + epithelial Na+ channel subunit
?
show the reaction diagram
-
Nedd4-2 binds to the PY motif of the subunit via its WW domains, ubiquitinates them and decreases their expression on the apical membrane
-
-
?
ATP + ubiquitin + Eps15
AMP + diphosphate + Eps15 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-1
-
-
?
ATP + ubiquitin + ERalpha
?
show the reaction diagram
P38398
monoubiquitination
-
-
?
ATP + ubiquitin + estrogen receptor alpha
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + estrogen receptor alpha lysine
AMP + diphosphate + estrogen receptor alpha N-ubiquityllysine
show the reaction diagram
O43791
SPOP from the Cullin3/SPOP ubiquitin E3 ligase complex is required for estrogen receptor alpha ubiquitination
-
-
?
ATP + ubiquitin + estrogen receptor beta
?
show the reaction diagram
-
E6-AP is involved in estrogen receptor beta degradation through the proteasome pathway, the E6-AP ubiquitin ligase is recruited to estrogen receptor beta in a phosphorylation-dependent manner through serines 94 and 106
-
-
?
ATP + ubiquitin + F-box and leucine-rich-repeat protein 14
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Far1 lysine
AMP + diphosphate + Far1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + gamma-tubulin
?
show the reaction diagram
P38398
monoubiquitination
-
-
?
ATP + ubiquitin + geminin
?
show the reaction diagram
P35226, Q64028, Q9CQJ4
PcG complex 1 acts as the E3 ubiquitin ligase for heminin
-
-
?
ATP + ubiquitin + geminin
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + gp78 lysine
AMP + diphosphate + gp78 N-ubiquityllysine
show the reaction diagram
-
auto-ubiquitination
-
?
ATP + ubiquitin + Hgs lysine
AMP + diphosphate + Hgs ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-1
-
-
?
ATP + ubiquitin + HHR23A
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + HHR23B
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + histone H2A
?
show the reaction diagram
P38398
monoubiquitination
-
-
?
ATP + ubiquitin + histone H2A lysine
AMP + diphosphate + histone H2A N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + histone H2A lysine
AMP + diphosphate + histone H2A N-ubiquityllysine
show the reaction diagram
P35226, Q64028, Q9CQJ4
monoubiquitination at Lys119
-
-
?
ATP + ubiquitin + histone H2AX
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + histone H2B
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + histone H3
?
show the reaction diagram
P38398
monoubiquitination
-
-
?
ATP + ubiquitin + histone H4
?
show the reaction diagram
P38398
monoubiquitination
-
-
?
ATP + ubiquitin + homeodomain-interacting protein kinase 2
?
show the reaction diagram
-
Siah-1 expression facilitates homeodomain-interacting protein kinase 2 polyubiquitination, degradation, and thereby inactivation
-
-
?
ATP + ubiquitin + Hsc70 lysine
AMP + diphosphate + Hsc70 N-ubiquityllysine
show the reaction diagram
-
activity in recombinant cells with full-length and various domains of Hsc70 as substrates, overview
-
-
?
ATP + ubiquitin + hScrib lysine
AMP + diphosphate + hScrib N-ubiquityllysine
show the reaction diagram
-
hScrib is the human homolog of the Drosophila Scribble, Vartul, tumor suppressor protein
-
?
ATP + ubiquitin + Hsp70 lysine
AMP + diphosphate + Hsp70 N-ubiquityllysine
show the reaction diagram
-
interaction analysis, activity in recombinant cells with full-length and various domains of Hsc70 as substrates, overview
-
-
?
ATP + ubiquitin + hypoxia-inducible factor 1alpha
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + inhibitor of beta-catenin and Tcf-4
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + interferone gamma receptor 1
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + intracellular adhesion molecule-1
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + IRF3 lysine
AMP + diphosphate + IRF3 N-ubiquityllysine
show the reaction diagram
-
Ro52 interacts with IRF3 via its C-terminal SPRY domain resulting in the polyubiquitination and proteasomal degradation, Ro52-mediated IRF3 degradation significantly inhibits IFN-beta promoter activity
-
-
?
ATP + ubiquitin + JPD2 lysine
AMP + diphosphate + JPD2 N-ubiquityllysine
show the reaction diagram
Q8IU81
IRF2-BP1 associates with and enhances the ubiquitination of JDP2
-
-
?
ATP + ubiquitin + K48 lysine
AMP + diphosphate + K48 N-ubiquityllysine
show the reaction diagram
-
Triad1 can catalyze the formation of Ub chains linked through both K48 and K63, recombinant ubiquitin substrate
-
-
?
ATP + ubiquitin + K63 lysine
AMP + diphosphate + K63 N-ubiquityllysine
show the reaction diagram
-
Triad1 can catalyze the formation of Ub chains linked through both K48 and K63, recombinant ubiquitin substrate
-
-
?
ATP + ubiquitin + KIAA0860 lysine
AMP + diphosphate + KIAA0860 N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + KLF2 lysine
AMP + diphosphate + KLF2 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + KLF5 lysine
AMP + diphosphate + KLF5 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Ku70 lysine
AMP + diphosphate + Ku70 N-ubiquityllysine
show the reaction diagram
-
interaction via the 80 kDa subunit of Ku protein heterodimer, Ku70 knockdown diminishes antiapoptotic activity of Akt, i.e. 70 kDa subunit of Ku protein heterodimer, interaction via the 80 kDa subunit of Ku protein heterodimer, a Nutlin-3-sensitive domain of Hdm2 is required for Ku70 destabilization, overview
-
-
?
ATP + ubiquitin + MAPK kinase 4 lysine
AMP + diphosphate + MAPK kinase 4 N-ubiquityllysine
show the reaction diagram
-
Itch ubiquitinates MAPK kinase 4 at lysines 140 and 143
-
-
?
ATP + ubiquitin + MBP-EL5 lysine
AMP + diphosphate + MBP-EL5 N-ubiquityllysine
show the reaction diagram
Q8S919
auto-ubiquitination of MBP moiety
-
?
ATP + ubiquitin + Mcl-1 lysine
AMP + diphosphate + Mcl-1 ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Mcl-1 lysine
AMP + diphosphate + Mcl-1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Mcl1 lysine
AMP + diphosphate + Mcl1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Mcm7
?
show the reaction diagram
Q05086
-
-
-
?
ATP + ubiquitin + Mdm2 lysine
AMP + diphosphate + Mdm2 N-ubiquityllysine
show the reaction diagram
-
Mdm2 mediates its own ubiquitination in a RING-finger dependent manner
-
?
ATP + ubiquitin + methylenebisphosphonate
AMP + diphosphate + ?
show the reaction diagram
-
-
-
-
r
ATP + ubiquitin + MHC class I-related chain A lysine
?
show the reaction diagram
P90489
E3 ubiquitin ligase K5 downregulates cell-surface expression of the natural killer cell group 2D ligands MHC class I-related chains A and B by ubiquitination of MIC cytoplasmic tail lysine residues
-
-
-
ATP + ubiquitin + Miz1 lysine
AMP + diphosphate + Miz1 N-ubiquityllysine
show the reaction diagram
-
Lys-48-linked polyubiquitination, Mule ubiquitinates GST-tagged Miz1 in the presence of ubiquitin (K48O) but not ubiquitin (K63O)
-
-
?
ATP + ubiquitin + MPP-EL5 lysine
AMP + diphosphate + MBP-EL5 N-ubiquityllysine
show the reaction diagram
Q8S919
auto-ubiquitination of MBP moiety
-
?
ATP + ubiquitin + Mre11 lysine
AMP + diphosphate + Mre11 N-ubiquityllysine
show the reaction diagram
-
degradation of this protein occurs via a cellular E3 ubiquitin ligase complex that is assembled through interactions between elongins B and C and BC boxes present in human adenovirus type 5 protein E4orf6 to form a cullin 5-based ligase complex
-
-
?
ATP + ubiquitin + N-CoR lysine
AMP + diphosphate + N-CoR N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + N-Myc lysine
AMP + diphosphate + N-Myc N-ubiquityllysine
show the reaction diagram
-
Huwe1 ubiquitinates the N-Myc oncoprotein through Lys48-mediated linkages and targets it for destruction by the proteasome, N-Myc is a better substrate for Huwe1 than c-Myc
-
-
?
ATP + ubiquitin + Nek2A
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + neurofilament light subunit lysine
AMP + diphosphate + neurofilament light subunit N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + NIX
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + NOTCH
?
show the reaction diagram
-
FBW7 targets NOTCH for ubiquitination and degradation
-
-
?
ATP + ubiquitin + Notch 1
?
show the reaction diagram
Q8VBV4
-
-
-
?
ATP + ubiquitin + Notch-1
AMP + diphosphate + Notch-1 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Notch-1 lysine
AMP + diphosphate + Notch-1 ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Nrf2 lysine
AMP + diphosphate + Nrf2 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + nuclear domain 10
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + nucleophosmin/B23
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + null Hong Kong variant of alpha1-antitrypsin
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + ORC1
?
show the reaction diagram
-
substrate of SCF and APC/C
-
-
?
ATP + ubiquitin + p21
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p21
?
show the reaction diagram
-
substrate of SCF and APC/C
-
-
?
ATP + ubiquitin + p21 lysine
AMP + diphosphate + p21 N-ubiquityllysine
show the reaction diagram
-
p21 associates with the CRL4Cdt2 ubiquitin ligase complex via Cdt2, proliferating cell nuclear antigen is required for the efficient ubiquitylation and degradation of p21
-
-
?
ATP + ubiquitin + p21Cip1 lysine
AMP + diphosphate + p21Cip1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p27
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p27
?
show the reaction diagram
-
substrate of SCF and Cul4A
-
-
?
ATP + ubiquitin + p27 lysine
AMP + diphosphate + p27 N-ubiquityllysine
show the reaction diagram
-
substrate of the E3 ligase Skp2
-
-
?
ATP + ubiquitin + p27 lysine
AMP + diphosphate + p27 N-ubiquityllysine
show the reaction diagram
-
ubiquitination and subsequent degradation of p27 is initiated by phosphorylation on Thr187 and is stimulated by p27's association with Cdk2-CycE
-
-
?
ATP + ubiquitin + p33 lysine
AMP + diphosphate + p33 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p33 lysine
AMP + diphosphate + p33 N-ubiquityllysine
show the reaction diagram
-
six-His-ubiquitin and purified recombinant maltose binding protein-p33 as substrate, pulldown assay with immobilized MBP-p33C, no activity with MBP alone
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
Q96PM5
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
Q18223
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
degradation of this protein occurs via a cellular E3 ubiquitin ligase complex that is assembled through interactions between elongins B and C and BC boxes present in human adenovirus type 5 protein E4orf6 to form a cullin 5-based ligase complex
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
O08759
E6-AP degrades tumor suppressor p53 in association with the E6 oncoprotein of the human papilloma virus, E6-AP not only enhances the degradation of p53 but also regulates the neuronal cell growth
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
E6-AP does not recognize p53 in the absence of the viral oncoprotein E6, E6:E6-AP-induced inactivation of p53 plays a role in the development of more than 90% of human cervical carcinomas
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
Q76N89
NEDL1 has an ability to enhance the transcriptional activity of p53 independent of its ubiquitin ligase activity, functional interaction of NEDL1 with p53 may contribute to the induction of apoptosis in cancerous cells bearing wild type p53
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
p53 also regulates Siah-1-dependent homeodomain-interacting protein kinase 2 degradation
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
P23804
substrate of the E3 ligase MDM2
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
inactivation of p53, after Hdm2 nuclear translocation
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
Q96PM5
Pirh2 inhibits tumor suppressor p53 function via ubiquitination and proteasomal degradation, MDM2 might also be involved
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
assay method development using fluorescence-labeled substrates
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
inactivation of p53
-
-
?
ATP + ubiquitin + p63 lysine
AMP + diphosphate + p63 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + p73 lysine
AMP + diphosphate + p73 ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + parkin
AMP + diphosphate + parkin N-ubiquityllysine
show the reaction diagram
Q9Y4X5
parkin ubiquitinates itself and promotes its own degradation
-
-
?
ATP + ubiquitin + Parkin-associated endothelin receptor-like receptor lysine
AMP + diphosphate + Parkin-associated endothelin receptor-like receptor N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Parkin-associated endothelin receptor-like receptor lysine
AMP + diphosphate + Parkin-associated endothelin receptor-like receptor N-ubiquityllysine
show the reaction diagram
-
the proline-rich domain of HRD1 is necessary to promote the degradation of Parkin-associated endothelin receptor-like receptor, while the transmembrane domain is necessary to transfer Parkin-associated endothelin receptor-like receptor from the endoplasmic reticulum to the cytosol
-
-
?
ATP + ubiquitin + PGC-1alpha
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + phospholipase C-gamma1
?
show the reaction diagram
Q3TTA7
-
-
-
?
ATP + ubiquitin + phosphorylated activating transcription factor 4 lysine
AMP + diphosphate + phosphorylated activating transcription factor 4 N-ubiquityllysine
show the reaction diagram
-
activating transcription factor 4 competes and inhibits binding of beta-catenin to beta-TrCP
-
-
?
ATP + ubiquitin + phosphorylated IkappaBalpha lysine
AMP + diphosphate + phosphorylated IkappaBalpha N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + phosphorylated p27
AMP + diphosphate + ubiquintinated phosphorylated p27
show the reaction diagram
-
Skp1, Skp2, and Cul1 polyubiquitinate p27 at Thr187, while KPC1 and KPC polyubiquitinated p27 at Ser10
-
-
?
ATP + ubiquitin + platelet integrin alphaIIbbeta3
?
show the reaction diagram
Q9P0P0
-
-
-
?
ATP + ubiquitin + PLK1
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + poly-sumoylated Siz2
?
show the reaction diagram
-
poly-sumoylated Siz2 is a preferred substrate of Slx5-Slx8
-
-
?
ATP + ubiquitin + Prc1
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + progesterone receptor-A
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + proliferating cell nuclear antigen lysine
AMP + diphosphate + proliferating cell nuclear antigen N-ubiquityllysine
show the reaction diagram
-
HLTF promotes the ubiquitin-Lys-63-linked polyubiquitination of proliferating cell nuclear antigen at the Lys-164 residue
-
-
?
ATP + ubiquitin + promyelocytic leukaemia protein
?
show the reaction diagram
O88846
RNF4 only ubiquitinates promyelocytic leukaemia protein when conjugated by SUMO-2
-
-
?
ATP + ubiquitin + protein
AMP + diphosphate + ubiquitin-protein
show the reaction diagram
-
-
-
-
-
ATP + ubiquitin + protein
AMP + diphosphate + ubiquitin-protein
show the reaction diagram
-
-
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
the formation of ubiquitin-protein conjugates is essential for cell viability
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
ubiquitin is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis. The ubiquitin-activating enzyme, E1, catalyzes the first step in ubiquitin conjugation
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
cellular proteins are marked for selective degradation by their ligation to the polypeptide ubiquitin
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
catalyzes the first step in ubiquitin conjugation
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
rate-limiting enzyme in the ubiquitin conjugation process
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
conjugation of multiple ubiquitins serves as a committed step in the degradation of a variety of intracellular eukaryotic proteins by the 26S proteasome. Conjugates are formed via a three-enzyme cascade. The initial step requires ubiquitin-activating enzyme, E1, which couples ubiquitin activation to ATP hydrolysis
-
-
-
ATP + ubiquitin + protein delta lysine
AMP + diphosphate + protein delta N-ubiquityllysine
show the reaction diagram
-
the ubiquitin E3 ligase binds to protein delta lysine through its first neuralized homology repeat 1, NHR1, domain and mediates the ubiquitination of protein delta lysine for endocytosis, endocytosis of protein delta lysine in the signal-sending cell is essential for Notch receptor activation, overview. Tom, a Bearded protein family member, inhibits the Neur-mediated endocytosis through interactions with the NHR1 domain. Neur NHR1 domain has binding activity to the 20-residue peptide corresponding to motif 2 of Tom
-
-
?
ATP + ubiquitin + protein kinase C theta
?
show the reaction diagram
Q3TTA7
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9C035
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P35131
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q8S919
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9CR50
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q96PM5
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9UTG2
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q5D267, Q99F15, Q9SVC6
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q969V5
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q16531
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9H992
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q8IYM9
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9LQ92
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q86XT4
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q810I2
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q13618
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9D5V5
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q96845
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
TRAF6 functions together with Ubc13/Uev1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine 63 of ubiquitin
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P15919
an intact RING domain of TRAF6 in conjunction with the E2 enzyme Ubc13/Uev1A is necessary for Lys-63-linked auto-ubiquitination of TRAF6 and for its ability to activate IkappaB kinase and NF-kappaB. TRAF6 serves as an E3 to directly ubiquitinate NEMO
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
DIAP1 (a caspase-antagoniting E3 ligase) is required for proper C4da neuron dendrite pruning. Activation of the ubiquitin-proteasome system likely leads to UbcD1-mediated (ubiquitin-conjugating enzyme E2) degradation of DIAP1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
ERalpha is predominantly monoubiquitinated in a reaction that involves interactions with both BRCA1 and BARD1. Identification of ERalpha as a putative BRCA1/BARD1 ubiquitination substrate reveals a potential link between the loss of BRCA1/BARD1 ligase activity and tissue-specific carcinoma
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
protein ubiquitination plays an important role in the immune responses. This process is catalyzed by a cascade of enzymatic reactions, with the E3 ubiquitin ligases being the critical enzymes that determine the specificity of substrate recognition
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
Pseudomonas syringae injects the AvrPtoB type III effector protein into the plant cell to suppress programmed cell death associated with plant immunity. AvrPtoB manipulates the host ubiquitin machinery and employs intrinsic E3 Ub ligase activity to suppress hypersensitive response-based programmed cell death and plant immunity
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
PUB17 ligase activity is crucial for defense signaling. Arabidopsis PUB17 knockout plants are compromised in RPM1- and RPS4-mediated resistance against Pseudomonas syringae pv tomato containing avirulence genes AvrB and AvrRPS4, respectively
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
the breast and ovarian specific tumor suppressor BRCA1, together with BARD1, comprise an E3 ubiquitin ligase. BRCA1 effects on transcription and DNA damage repair can be mediated via the ubiquitin ligase activities directed at RNA polymerase II or topo IIalpha. The effects of BRAC1 on genomic stability in breast cells can be mediated by ubiquitination of centrosome proteins, including gamma-tubulin and Npm1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
the ubiquitin ligase complex SCFFbs1 contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
TRIM25 E3 ubiquitin ligase induces the Lys 63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
ubiquitin-protein E6AP is extensively involved in the ubiquitin mediated degradation of high-risk human papillomavirus E6-dependent substrates as a cellular E3 ubiquitin-protein ligase
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
diffusion-driven mechanism for substrate ubiquitination by the SCF-Cdc34 ubiquitin ligase complex
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
mouse Ube2g2 (conjugating enzyme E2) and human gp78 (ligase E3), an endoplasmic reticulum (ER)-associated conjugating system essential for the degradation of misfolded ER proteins, are recombinantly expressed in Escherichia coli. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Intriguingly, polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P90495
in addition to lysine ubiquitination, MIR1 has the unique ability of transferring ubiquitin onto MHC-I molecules lacking available lysine residues, in a cysteine-dependent manner, MIR1 activity is maximal when either a lysine or cysteine residue is placed approximately 15 amino acids away from the transmembrane domain, MIR2 preferentially targets residues, including cysteines, that are closer than 15 amino acids to the transmembrane domain
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q5RJY2
the protein has a catalytically inactive HECT domain and two distinct RING-like ubiquitin ligase domains that catalyze lysine 48-linked polyubiquitination
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P38398
ubiquitin and BRCA1 likely affect each other in many ways to perform cellular functions
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
E3 mediates the ubiquitin transfer from E2, the ubiquitin-conjugating enzyme to the lysine residue(s) of the substrate
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
substrate specificity of SCF ubiquitin ligase is altered by the substrate recognition subunit F-box protein
-
-
?
ATP + ubiquitin + protein p53
?
show the reaction diagram
Q00987
-
-
-
?
ATP + ubiquitin + PRP19 lysine
AMP + diphosphate + PRP19 N-ubiquityllysine
show the reaction diagram
-
auto ubiquitination
-
?
ATP + ubiquitin + PTEN
?
show the reaction diagram
-
Nedd4-1-mediates polyubiquitination of PTEN and regulates its stability and nuclear localization
-
-
?
ATP + ubiquitin + PTEN lysine
AMP + diphosphate + PTEN N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-1
-
-
?
ATP + ubiquitin + RCAN1
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + receptor interacting protein lysine
AMP + diphosphate + receptor interacting protein N-ubiquityllysine
show the reaction diagram
Q8WZ73
-
-
-
?
ATP + ubiquitin + RhoA lysine
AMP + diphosphate + RhoA N-ubiquityllysine
show the reaction diagram
Q9HCE7
-
-
-
?
ATP + ubiquitin + RPB1
?
show the reaction diagram
P38398
RPB1 is the largest subunit of RNA polymerase II
-
-
?
ATP + ubiquitin + RPB8
?
show the reaction diagram
P38398
RPB8 is the common subunit of RNA polymerase
-
-
?
ATP + ubiquitin + RPN12a lysine
AMP + diphosphate + RPN12a N-ubiquityllysine
show the reaction diagram
Q84TG3, Q9SVC6
PUB22 physically interacts with RPN12a, a subunit of the 19S regulatory particle in the 26S proteasome
-
-
?
ATP + ubiquitin + RPN12a lysine
AMP + diphosphate + RPN12a N-ubiquityllysine
show the reaction diagram
Q84TG3, Q9SVC6
PUB23 physically interacts with RPN12a, a subunit of the 19S regulatory particle in the 26S proteasome
-
-
?
ATP + ubiquitin + Runx2 lysine
AMP + diphosphate + Runx2 N-ubiquityllysine
show the reaction diagram
Q9CUN6
-
-
-
?
ATP + ubiquitin + Runx2 lysine
AMP + diphosphate + Runx2 N-ubiquityllysine
show the reaction diagram
Q9HCE7
-
-
-
?
ATP + ubiquitin + S6 kinase 1
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Sec61-2L
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Sec7 lysine
AMP + diphosphate + Sec7 N-ubiquityllysine
show the reaction diagram
-
binding of Sec7 to Rsp5 is dependent on the presence of the phosphoinositide 3-kinase Vps34, suggesting that phosphatidylinositol 3-phosphate plays a role in regulating this interaction
-
-
?
ATP + ubiquitin + securin
?
show the reaction diagram
-
he APC/C E3 ligase is required to trigger the metaphase to anaphase transition by degrading the separase inhibitor securin
-
-
?
ATP + ubiquitin + securin lysine
AMP + diphosphate + securin N-ubiquityllysine
show the reaction diagram
Q94297
-
-
-
?
ATP + ubiquitin + Sic1 lysine
AMP + diphosphate + Sic1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Skp1 lysine
AMP + diphosphate + Skp1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Skp2
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + Smad 5 lysine
AMP + diphosphate + Smad 5 N-ubiquitinyllysine
show the reaction diagram
A2A5Z6
-
-
-
?
ATP + ubiquitin + Smad1 lysine
AMP + diphosphate + Smad1 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
CHIP decreases the protein levels of Smad1 in a ligand-independent manner
-
-
?
ATP + ubiquitin + Smad1 lysine
AMP + diphosphate + Smad1 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smurf2 ubiquitinates Smad1 in a ligand-independent manner
-
-
?
ATP + ubiquitin + Smad1 lysine
AMP + diphosphate + Smad1 N-ubiquityllysine
show the reaction diagram
Q9CUN6
-
-
-
?
ATP + ubiquitin + Smad1 lysine
AMP + diphosphate + Smad1 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad1/5 lysine
AMP + diphosphate + Smad1/5 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smurf1 ubiquitinates Smad1/5 in a ligand-independent manner
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
-
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Nedd4-2 is capable of enhancing the ubiquitination and degradation of Smad2 in the presence of activated TbetaR-I
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smad2 ubiquitination by WWP1 may require TGFB-induced factor
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-2
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
the interaction between Smad2 and Itch is enhanced after TGF-beta ligation
-
-
?
ATP + ubiquitin + Smad2 lysine
AMP + diphosphate + Smad2 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad3 lysine
AMP + diphosphate + Smad3 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
CHIP decreases total Smad3 levels independent of TGF-beta activation
-
-
?
ATP + ubiquitin + Smad3 lysine
AMP + diphosphate + Smad3 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
ROC1-SCFFbw1a interacts with Smad3 to induce the degradation of Smad3 in a ligand-dependent manner
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
-
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
CHIP decreases the protein levels of Smad4 in a ligand-independent manner
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
ectodermin binds to Smad4 and may induce Smad4 ubiquitination and degradation
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
SCFFbw1a decreases Smad4 protein stability
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smad7 functions as an adaptor for Nedd4-2 in the ubiquitination of Smad4
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smad7 functions as an adaptor for WWP1 in the ubiquitination of Smad4
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-2
-
-
?
ATP + ubiquitin + Smad4 lysine
AMP + diphosphate + Smad4 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad5 lysine
AMP + diphosphate + Smad5 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad5 lysine
AMP + diphosphate + Smad5 N-ubiquityllysine
show the reaction diagram
Q9HCE7
-
-
-
?
ATP + ubiquitin + Smad7 lysine
AMP + diphosphate + Smad7 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
axin cooperates with Arkadia to reduce Smad7 stability
-
-
?
ATP + ubiquitin + Smad7 lysine
AMP + diphosphate + Smad7 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smurf1 induces the export of Smad7 from the nucleus to the cytoplasm, allowing Smad7 to associate with activated type I receptors at the plasma membrane
-
-
?
ATP + ubiquitin + Smad7 lysine
AMP + diphosphate + Smad7 N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smurf2 induces the degradation of Smad7 and the associated receptors
-
-
?
ATP + ubiquitin + SnoN lysine
AMP + diphosphate + SnoN N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Smurf2 interacts with SnoN via Smad2 to induce ubiquitin-dependentdegradation of SnoN in response to TGF-beta
-
-
?
ATP + ubiquitin + SnoN lysine
AMP + diphosphate + SnoN N-ubiquityllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
APC uses Smad3 as an adaptor for SnoN recruitment to induce the ubiquitin-dependent degradation of SnoN
-
-
?
ATP + ubiquitin + SnoN lysine
AMP + diphosphate + SnoN N-ubiquityllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Arkadis enhances TGF-beta signaling by inducing ubiquitin-dependent degradation of SnoN
-
-
?
ATP + ubiquitin + sorting nexin 9 lysine
AMP + diphosphate + sorting nexin 9 N-ubiquityllysine
show the reaction diagram
-
Itch ubiquitylates and regulates the level of sorting nexin 9
-
-
?
ATP + ubiquitin + Spd1 lysine
AMP + diphosphate + Spd1 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + spine-associated Rap GTPase activating protein
?
show the reaction diagram
-
degradation of spine-associated Rap GTPase activating protein by SCFbeta-TRCP depends on the activity-inducible protein kinase Polo-like kinase 2
-
-
?
ATP + ubiquitin + sterol regulatory element binding protein 1
?
show the reaction diagram
-
substrate of isoform Fbw7alpha
-
-
?
ATP + ubiquitin + survivin
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + synaptotagmin XI
AMP + diphosphate + synaptotagmin XI N-ubiquityllysine
show the reaction diagram
-
parkin binds to the C2A and C2B domains of synaptotagmin XI resulting in the polyubiquitination of synaptotagmin XI. The interaction with this protein suggests a role for parkin in the regulation of the synaptic vesicle pool and in vesicle release. Loss of parkin could affect multiple proteins controlling vesicle pools, docking and release and explain the deficits in dopaminergic function seen in patients with parkin mutations, parkin binds to the C2A and C2B domains of synaptotagmin XI resulting in the polyubiquitination of synaptotagmin XI
-
-
?
ATP + ubiquitin + syntaxin 1 lysine
AMP + diphosphate + syntaxin 1 N-ubiquityllysine
show the reaction diagram
Q8CJB9
staring targets syntaxin 1 for proteasome dependent degradation
-
?
ATP + ubiquitin + TbetaR-I lysine
AMP + diphosphate + TbetaR-I N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
-
-
-
?
ATP + ubiquitin + TbetaR-I lysine
AMP + diphosphate + TbetaR-I N-ubiquitinyllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
WWP1 interacts with TbetaR-I via Smad7 to induce ubiquitin-dependent degradation of TbetaR-I
-
-
?
ATP + ubiquitin + TbetaR-I lysine
AMP + diphosphate + TbetaR-I N-ubiquityllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
-
-
-
?
ATP + ubiquitin + TbetaR-I lysine
AMP + diphosphate + TbetaR-I N-ubiquityllysine
show the reaction diagram
Q6ZNA4, Q96J02, Q96PU5
Nedd4-2 interacts with TbetaR-I via Smad7 to induce ubiquitin-dependent degradation of TbetaR-I
-
-
?
ATP + ubiquitin + TbetaR-I/II lysine
AMP + diphosphate + TbetaR-I/II N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + TbetaR-I/II lysine
AMP + diphosphate + TbetaR-I/II N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + TbetaR-I/II lysine
AMP + diphosphate + TbetaR-I/II N-ubiquitinyllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
substrate of isozyme Nedd4-2
-
-
?
ATP + ubiquitin + TIEG lysine
AMP + diphosphate + TIEG N-ubiquityllysine
show the reaction diagram
Q8IUQ4
-
-
-
?
ATP + ubiquitin + Tome-1
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + TopBP1 lysine
AMP + diphosphate + TopBP1 N-ubiquityllysine
show the reaction diagram
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0, Q9HAU4, Q9HCE7
-
-
-
?
ATP + ubiquitin + TPX2
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + transcription factor IIE
?
show the reaction diagram
P38398
-
-
-
?
ATP + ubiquitin + transforming growth factor-beta-inducible early gene 1 product
?
show the reaction diagram
Q8C863
Itch associates with and promotes conjugation of ubiquitin to the transcription factor transforming growth factor-beta-inducible early gene 1 product , Itch cooperates with transforming growth factor-beta-inducible early gene 1 product to induce Foxp3 expression
-
-
?
ATP + ubiquitin + Tribbles 3 homolog lysine
AMP + diphosphate + Tribbles 3 homolog N-ubiquityllysine
show the reaction diagram
Q8IUQ4
SIAH1 targets Tribbles 3 homolog for proteasome-dependent degradation
-
-
?
ATP + ubiquitin + Trio-associated repeat on actin
?
show the reaction diagram
Q5T447
HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Trio-associated repeat on actin
-
-
?
ATP + ubiquitin + truncated C-terminal portion of Bid lysine
AMP + diphosphate + truncated C-terminal portion of Bid N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Tsg101 lysine
AMP + diphosphate + Tsg101 N-ubiquityllysine
show the reaction diagram
-
substrate of the E3 ligases mahogunin and Tal
-
-
?
ATP + ubiquitin + tubby like protein 3
?
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + tuberin lysine
AMP + diphosphate + tuberin N-ubiquityllysine
show the reaction diagram
-
tuberin ubiquitination is independent of its phosphorylation by Akt, RSK1, and ERK kinases, the TSC1 protein hamartin, which forms a heterodimer with tuberin, protects tuberin from ubiquitination by Pam
-
-
?
ATP + ubiquitin + tuberous sclerosis complex 2 lysine
AMP + diphosphate + tuberous sclerosis complex 2 N-ubiquityllysine
show the reaction diagram
-
E6AP promotes the ubiquitination of tuberous sclerosis complex 2 independent of HPV16 E6
-
-
?
ATP + ubiquitin + Tweety homologue 2 lysine
AMP + diphosphate + Tweety homologue 2 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + Tweety homologue 3 lysine
AMP + diphosphate + Tweety homologue 3 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + UBA1 lysine
AMP + diphosphate + UBA1 N-ubiquityllysine
show the reaction diagram
P35131
recombinant enzyme
-
-
?
ATP + ubiquitin + UBC8 lysine
AMP + diphosphate + UBC8 N-ubiquityllysine
show the reaction diagram
P35131
recombinant enzyme
-
-
?
ATP + ubiquitin + UbcH5a lysine
AMP + diphosphate + UbcH5a N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + UbcH5b lysine
AMP + diphosphate + UbcH5b N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + UbcH7 lysine
AMP + diphosphate + UbcH7 N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + UbcH7 protein lysine
AMP + diphosphate + UbcH7 protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
Q16531
-
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
Q00987
-
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
Q13618
-
-
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
the COOH-terminal Gly of ubiquitin is activated by the enzyme
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
both an enzyme-bound COOH-terminal ubiquitin thiolester and a COOH-terminal ubiquitin adenylate are formed
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
reaction sequence: 1. initial formation of tightly enzyme-bound ubiquitin adenylate with diphosphate formation from ATP, 2. conversion of this intermediate to form AMP and a covalent enzyme-ubiquitin thiolester, 3. activation of a second molecule of ubiquitin to give a ternary complex of one equivalent each of ubiquitin thiolester and tightly bound ubiquitin adenylate per subunit of enzyme
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
the ubiquitin-activating enzyme E1 catalyzes the formation of ubiquitin adenylate and the transfer of activated ubiquitin to a thiol site on the same enzyme. Following activation, ubiquitin is transferred from the thiol site of E1 to the thiol site of one of several ubiquitin carrier proteins, known as E2s
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
a covalent possibly thioester intermediate, is formed between the activating anzyme and ubiquitin
-
-
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
Escherichia coli AR58
-
-
-
-
ATP + ubiquitin + UFD2a lysine
AMP + diphosphate + UFD2a N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + UFD2b lysine
AMP + diphosphate + UFD2b N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + UT-A1 urea transporter
?
show the reaction diagram
Q00987
-
-
-
?
ATP + ubiquitin + vanillin
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + Wee1A
?
show the reaction diagram
-
substrate of SCF
-
-
?
ATP + ubiquitin + Xkid
?
show the reaction diagram
-
substrate of APC/C
-
-
?
ATP + ubiquitin + Zic2 lysine
AMP + diphosphate + Zic2 N-ubiquityllysine
show the reaction diagram
Q3U827
-
-
-
?
ATP + ubiquitin + [epidermal growth factor receptor ErbB3]
AMP + diphosphate + [epidermal growth factor receptor ErbB3]-N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + [epidermal growth factor receptor ErbB3]
AMP + diphosphate + [epidermal growth factor receptor ErbB3]-N-ubiquityllysine
show the reaction diagram
Q9H4P4
Nrdp1 may act to regulate steady-ste cell surface neuregulin receptor levels, thereby influencing the efficiency of neuregulin signaling
-
-
?
ATP + ubiquitin + [epidermal growth factor receptor ErbB3]
AMP + diphosphate + [epidermal growth factor receptor ErbB3]-N-ubiquityllysine
show the reaction diagram
-
Nrdp1 promotes degradation of Erb3 by proteasomes and thus, may be an important factor influencing cell growth
-
-
?
ATP + ubiquitin + [p38 subunit of the aminoacyl-tRNA synthetase complex]
AMP + diphosphate + [p38 subunit of the aminoacyl-tRNA synthetase complex] N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + [unfolded Pael receptor]
AMP + diphosphate + [unfolded Pael receptor] N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
ability to enhance the ubiquitination and degradation of misfolded tubulins may play a significant role in protecting neurons from toxins that cause Parkinson‘s disease
-
-
-
additional information
?
-
Q9Y4X5
parkin functions as an E3 ubiquitin-protein ligase through its ring domains and may control protein levels via ubiquitination. The loss of Parkin‘s ubiquitin-protein ligase function in familial-linked mutations suggests that this may be the cause of familial autosomal recessive Parkinson‘s disease
-
-
-
additional information
?
-
-
each of the three PHD fingers of Msc1 can act as ubiquitin E3 ligases
-
-
-
additional information
?
-
-
Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases
-
-
-
additional information
?
-
-
ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia-mutated and Rad3-related (ATR) phosphorylate Siah-1 at Ser19 to modulate HIPK2-Siah-1 interaction
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Bmi1 performs self-ubiquitination
-
-
-
additional information
?
-
P38398
BRCA1 and BARD1 are required to recruit Rad51 and BRCA2, the central players in homologous recombination at damaged DNA sites, BRCA1-BARD1 in the Pol II-holoenzyme may act as a transcriptional co-activator
-
-
-
additional information
?
-
Q8WZ73
CARP-2 inhibits tumor necrosis factor-induced IkappaB kinase activation and IkappaBalpha degradation
-
-
-
additional information
?
-
-
Cbl-b associates with protein kinase C-theta upon T-cell antigen receptor stimulation and regulates T-cell antigen receptor-induced protein kinase C-theta activation via Vav-1, which couples protein kinase C-theta to phosphatidylinositol 3-kinase and allows it to be phosphorylated, the down-regulation of -cell antigen receptor-induced NF-kappaB activation by Cbl-b is mediated coordinately by both Akt-dependent and protein kinase C-theta-dependent signaling pathways in primary T cells
-
-
-
additional information
?
-
Q3TTA7
Clb-b plays a predominant role in peripheral T-cell activation and is critically involved in T-cell exhaustion and prevention of lethal disease at infection with murine lymphocytic choriomeningitis virus, Cbl-b ubiquitin ligase regulates virus-specific CD8+ T-cell expansion and antiviral function during acute and chronic infections
-
-
-
additional information
?
-
Q9H992
deubiquitylation is responsible for the USP9Xand USP7-mediated stabilization of MARCH7
-
-
-
additional information
?
-
Q6ZNA4, Q96J02, Q96PU5
E3 ubiquitin ligases catalyze the ubiquitination of both their substrates and themselves, in many cases, E3 autoubiquitination induces their proteasome-dependent degradation, suggesting that autoubiquitination controls E3 ubiquitin ligase abundance
-
-
-
additional information
?
-
Q5RJY2
G2E3 is capable of autoubiquitination
-
-
-
additional information
?
-
Q7Z6Z7
HUWE1 is implicated in TP53-associated regulation of the neuronal cell cycle
-
-
-
additional information
?
-
-
interaction of human immunodeficiency virus type 1 Vif with the cullin 5 ubiquitin ligase is required for cell cycle disruption
-
-
-
additional information
?
-
Q9H992
MARCH7 readily undergoes autoubiquitylation
-
-
-
additional information
?
-
Q00987
MDM2 is capable of autoubiquitination and degradation via the proteasome
-
-
-
additional information
?
-
P46934
Nedd4 inhibits PTEN-induced apoptotic cell death
-
-
-
additional information
?
-
-
Pam F3 is ubiquitinated when co-transfected with Ub-HA in HEK293T cells
-
-
-
additional information
?
-
Q13618
potassium channel tetramerization domain 5 is a substrate-specific adaptor for cullin3-based E3 ligases
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Rae28 impairs ubiquitin-proteasome-mediated degradation of geminin and increases protein stability, Rae28 mediates recruiting Scmh1, which provides PcG complex 1 an interaction domain for geminin
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Rae28 performs self-ubiquitination
-
-
-
additional information
?
-
Q68DV7
recombinant RNF43 fused with maltose-binding protein has autoubiquitylation activity
-
-
-
additional information
?
-
Q3U827
Rines is heavily ubiquitinated and degraded by proteasome
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Ring1B performs self-ubiquitination
-
-
-
additional information
?
-
Q9P0P0
RN181 is a ubiquitin E3 ligase which is self-ubiquitinated as part of the enzymatic reaction, RN181 plays a role for ubiquitination in integrin and platelet signalling
-
-
-
additional information
?
-
O88846
RNF4 undergoes auto-ubiquitination, RNF4 preferentially binds poly-SUMO-2 chains with relatively weak binding to mono- or di-SUMO
-
-
-
additional information
?
-
-
Siah1 does not promote P-glycoprotein degradation but decreases its expression transcriptionally by promoting c-Jun transcription factor binding to the activator protein 1 (AP1) site in the MDR1 promoter, blocking proteasome function by MG132 does not prevent the downregulation of P-glycoprotein by Siah1, Siah1 triggers c-Jun NH2-terminal kinase activation, leading to enhanced phosphorylation of c-Jun, and the JNK/c-Jun signalling axis is critical for Siah1 to down-regulate MDR1/P-glycoprotein expression
-
-
-
additional information
?
-
-
Skp1a is a cellular interaction partner of the 68k ankyrin-like protein from orthopoxviruses, SCF complex formation is likely to have a functional role during orthopoxviral infections
-
-
-
additional information
?
-
Q96845
the active-site cysteine of the HECT domain is critical for association of Nedd4-2s with virus-like particles
-
-
-
additional information
?
-
-
the CUL4-DDB1 ubiquitin ligase interacts with Raptor and regulates the mTORC1-mediated signaling pathway through ubiquitin-dependent proteolysis
-
-
-
additional information
?
-
-
the E3 ligase is required to trigger the metaphase to anaphase transition by degrading the separase inhibitor securin
-
-
-
additional information
?
-
-
the herpes simplex virus UL56 protein is not ubiquitinated despite its interaction with Nedd4
-
-
-
additional information
?
-
-
the poxvirus Orf virus use ankyrin repeat protein 008 to dictate target specificity to SCF1 ubiquitin ligase and thereby exploit the cell's ubiquitin-proteasome machinery, the protein interacts with the SCF1 complex component SKP1 in an F-box-dependent but ankyrin-independent manner
-
-
-
additional information
?
-
Q9C035
TRIM5alpha does not ubiquitinate Ro52, TRIM5alpha ubiquitinates itself and is also ubiquitinated by the E3 ubiquitin ligase Ro52, is deubiquitinated by YopJ (one of the pathogenic proteins derived from Yersinia species), and selectively deubiquitinated by UnpEL, the ubiquitination of TRIM5a does not lead to its proteasomal degradation in HeLa cells
-
-
-
additional information
?
-
-
VprBP strongly associates with DDB1-CUL4 and binds to chromatin and functions by either recruiting specific substrates or promoting the recruitment of other substrates to the DDB1-CUL4-ROC1 E3 ligase
-
-
-
additional information
?
-
-
activity towards Notch is barely detected
-
-
-
additional information
?
-
P38398
BRCA1 autoubiquitinates when bound to BARD1, and the ubiquitination stabilizes BRCA1 and enhances its E3 activity
-
-
-
additional information
?
-
A2A5Z6
casein kinase-2 interacting protein-1 is not a substrate of Smurf1
-
-
-
additional information
?
-
-
clodronate, etidronate, and pamidronate are no substrates
-
-
-
additional information
?
-
-
does not bind Tweety homologue 1
-
-
-
additional information
?
-
Q68DV7
HAP95 interacts with RNF43 in the nucleus, but HAP95 is unlikely to serve as a substrate of RNF43 ubiquitin ligase
-
-
-
additional information
?
-
-
Hul5 activity promotes the interaction of the truncated CTLmyc with the AAA-ATPase Cdc48
-
-
-
additional information
?
-
-
Kf-1 interacts with components of the endoplasmic reticulum-associated degradation pathway, including Derlin-1 and VCP
-
-
-
additional information
?
-
P19812
UBR1 binds to either type-1 or type-2 destabilizing N-terminal residues of reporter peptides but does not bind to a stabilizing N-terminal residue such as Gly
-
-
-
additional information
?
-
-
UFD2a is degraded by cisplatin
-
-
-
additional information
?
-
-
unsumoylated Siz2 and multi-sumoylated Siz2 are no substrates
-
-
-
additional information
?
-
-
E6-AP induces ubiquitination and degradation of misfolded luciferases that are bound with Hsp70
-
-
-
additional information
?
-
-
Fbw7, the substrate recognition subunit of SCFFbw7 ubiquitin ligase, facilitates the degradation of several proto-oncogene products by the proteasome
-
-
-
additional information
?
-
Q96PM5
isozymes Pirh2B and Pirh2C can downregulate p53 protein levels and promote intracellular p53 ubiquitination
-
-
-
additional information
?
-
-
recombinant REDD1 is rapidly degraded by the ubiquitin-proteasome system mediated by the CUL4A-DDB1-ROC1-beta-TRCP E3 ligase complex, i.e. cullin 4A-DNA damage-binding protein 1-regulator of cullins 1-ubiquitin ligase complex, through glycogen synthase kinase 3-beta phosphorylation and beta-transducin repeat-containing protein activity, overview
-
-
-
additional information
?
-
-
recombinant Triad1 inhibits clonogenic growth and proliferation of transfected U937 cells, requiring the RING fingers, without affecting their differentiation and apoptosis
-
-
-
additional information
?
-
-
Rsp5p inhibits Tomato bushy stunt virus, TBSV, replication in recombinant Saccharomyces cerevisiae by direct binding between Rsp5p and p92pol reducing the stability of p92pol, interaction analysis, overview. The WW domain of Rsp5p, which is involved in protein interactions, is responsible for inhibition of TBSV replication, whereas the HECT domain, involved in protein ubiquitination, is not necessary for Rsp5p-mediated inhibition of viral replication
-
-
-
additional information
?
-
O60291
MGRN1 isoforms inhibit cAMP production in HEK-293T cells expressing MC1R or MC4R, and in human melanoma cells expressing endogenous MC1R, overview
-
-
-
additional information
?
-
Q96PM5
Pirh2A associates with itself and with Pirh2B but not Pirh2C. Pirh2A performs also autoubiquitination. Pirh2A interacts directly with MDM2 within a region encompassing MDM2 acidic and zinc finger domains, Pirh2A associates with itself and with Pirh2B but not Pirh2C. Pirh2B isoform cannot undergo autoubiquitination and shows increased protein stability. Pirh2B interacts directly with MDM2 within a region encompassing MDM2 acidic and zinc finger domains, Pirh2A associates with itself and with Pirh2B but not Pirh2C. Pirh2C isoform cannot undergo autoubiquitination and shows increased protein stability. Pirh2C interacts directly with MDM2 within a region encompassing MDM2 acidic and zinc finger domains
-
-
-
additional information
?
-
-
the E3 ligase performs autoubiquitinylation
-
-
-
additional information
?
-
-
Triad1 catalyses both K48 and K63 linked polyubiquitination. Triad1 binds two different ubiquitin-conjugating enzymes, RING1 of Triad1 is essential for interaction with UbcH7 and RING2 for Ubc13
-
-
-
additional information
?
-
-
NleL preferentially catalyzes formation of unanchored polyubiquitin chains using Lys6 and Lys48 linkage
-
-
-
additional information
?
-
-
no activity with intact Bid protein
-
-
-
additional information
?
-
-
recombinant Ube2 is capable of making a thiolester bond with ubiquitin
-
-
-
additional information
?
-
-
the BRCA1-BARD1 RING complexes in the presence of ATP, ubiquitin, E1, and UbcH5c exhibit E3 ubiquitin ligase enzyme activity that promote the formation of high molecular weight polyubiquitin species that is significantly greater than those produced by the individual BRCA1 or BARD1 RING domain
-
-
-
additional information
?
-
Q9HCE7
the E3 activity of Smurf1 is not restricted by autoinhibition
-
-
-
additional information
?
-
Saccharomyces cerevisiae ATCC 204508
-
UBR1 binds to either type-1 or type-2 destabilizing N-terminal residues of reporter peptides but does not bind to a stabilizing N-terminal residue such as Gly
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + ubiquitin + alpha-synphilin-1
AMP + diphosphate + alpha-synphilin-1 N-ubiquityllysine
show the reaction diagram
-
parkin ubiquitinates proteins contained within cytosolic Lewy-body-like inclusions that contain both synphilin and alpha-synuclein. Familial-linked mutations in parkin impair the ubiquitination of proteins within these cytosolic inclusions
-
-
?
ATP + ubiquitin + alpha-synuclein
AMP + diphosphate + alpha-synuclein N-ubiquityllysine
show the reaction diagram
-
22000 Da glycosylated form of alpha-synuclein, the reaction is altered in Parkinson‘s disease, the loss of parkin function causes pathological accumulation of alpha-synuclein
-
-
?
ATP + ubiquitin + B7.2
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + c-myb lysine
AMP + diphosphate + c-myb N-ubiquityllysine
show the reaction diagram
-
substrate of SCFFbw7
-
-
?
ATP + ubiquitin + c-Myc lysine
AMP + diphosphate + c-Myc N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + CD166
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + CD1d
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + CD3-delta lysine
AMP + diphosphate + CD3-delta N-ubiquityllysine
show the reaction diagram
-
gp78 targets CD3-delta for proteasomal degradation
-
?
ATP + ubiquitin + CD31
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + CHIP lysine
AMP + diphosphate + CHIP N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + CYC4 lysine
AMP + diphosphate + CYC4 N-ubiquityllysine
show the reaction diagram
-
auto ubiquitination
-
?
ATP + ubiquitin + cyclin E
AMP + diphosphate + cyclin E N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + cyclin E lysine
AMP + diphosphate + cyclin E N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + EGR2 lysine
AMP + diphosphate + EGR2 N-ubiquityllysine
show the reaction diagram
Mus musculus, Mus musculus C57BL/6
-
AIP2 promotes EGR2 ubiquitination and degradation. AIP2 interacts with and promotes ubiquitin-mediated degradation of EGR2, a zinc finger transcription factor that regulates Fas ligand expression during activation-induced T-cell death
-
-
?
ATP + ubiquitin + geminin
?
show the reaction diagram
P35226, Q64028, Q9CQJ4
PcG complex 1 acts as the E3 ubiquitin ligase for heminin
-
-
?
ATP + ubiquitin + gp78 lysine
AMP + diphosphate + gp78 N-ubiquityllysine
show the reaction diagram
-
auto-ubiquitination
-
?
ATP + ubiquitin + interferone gamma receptor 1
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + intracellular adhesion molecule-1
?
show the reaction diagram
P90489
-
-
-
?
ATP + ubiquitin + KIAA0860 lysine
AMP + diphosphate + KIAA0860 N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + Ku70 lysine
AMP + diphosphate + Ku70 N-ubiquityllysine
show the reaction diagram
-
interaction via the 80 kDa subunit of Ku protein heterodimer, Ku70 knockdown diminishes antiapoptotic activity of Akt
-
-
?
ATP + ubiquitin + MBP-EL5 lysine
AMP + diphosphate + MBP-EL5 N-ubiquityllysine
show the reaction diagram
Q8S919
auto-ubiquitination of MBP moiety
-
?
ATP + ubiquitin + Mdm2 lysine
AMP + diphosphate + Mdm2 N-ubiquityllysine
show the reaction diagram
-
Mdm2 mediates its own ubiquitination in a RING-finger dependent manner
-
?
ATP + ubiquitin + MHC class I-related chain A lysine
?
show the reaction diagram
P90489
E3 ubiquitin ligase K5 downregulates cell-surface expression of the natural killer cell group 2D ligands MHC class I-related chains A and B by ubiquitination of MIC cytoplasmic tail lysine residues
-
-
-
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
-
inactivation of p53, after Hdm2 nuclear translocation
-
-
?
ATP + ubiquitin + p53 lysine
AMP + diphosphate + p53 N-ubiquityllysine
show the reaction diagram
Q96PM5
Pirh2 inhibits tumor suppressor p53 function via ubiquitination and proteasomal degradation, MDM2 might also be involved
-
-
?
ATP + ubiquitin + phosphorylated IkappaBalpha lysine
AMP + diphosphate + phosphorylated IkappaBalpha N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein
?
show the reaction diagram
-
the formation of ubiquitin-protein conjugates is essential for cell viability
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
ubiquitin is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis. The ubiquitin-activating enzyme, E1, catalyzes the first step in ubiquitin conjugation
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
cellular proteins are marked for selective degradation by their ligation to the polypeptide ubiquitin
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
catalyzes the first step in ubiquitin conjugation
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
rate-limiting enzyme in the ubiquitin conjugation process
-
-
-
ATP + ubiquitin + protein
?
show the reaction diagram
-
conjugation of multiple ubiquitins serves as a committed step in the degradation of a variety of intracellular eukaryotic proteins by the 26S proteasome. Conjugates are formed via a three-enzyme cascade. The initial step requires ubiquitin-activating enzyme, E1, which couples ubiquitin activation to ATP hydrolysis
-
-
-
ATP + ubiquitin + protein delta lysine
AMP + diphosphate + protein delta N-ubiquityllysine
show the reaction diagram
-
the ubiquitin E3 ligase binds to protein delta lysine through its first neuralized homology repeat 1, NHR1, domain and mediates the ubiquitination of protein delta lysine for endocytosis, endocytosis of protein delta lysine in the signal-sending cell is essential for Notch receptor activation, overview. Tom, a Bearded protein family member, inhibits the Neur-mediated endocytosis through interactions with the NHR1 domain. Neur NHR1 domain has binding activity to the 20-residue peptide corresponding to motif 2 of Tom
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P35131
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9CR50
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q96PM5
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
Q9UTG2
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
P15919
an intact RING domain of TRAF6 in conjunction with the E2 enzyme Ubc13/Uev1A is necessary for Lys-63-linked auto-ubiquitination of TRAF6 and for its ability to activate IkappaB kinase and NF-kappaB. TRAF6 serves as an E3 to directly ubiquitinate NEMO
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
DIAP1 (a caspase-antagoniting E3 ligase) is required for proper C4da neuron dendrite pruning. Activation of the ubiquitin-proteasome system likely leads to UbcD1-mediated (ubiquitin-conjugating enzyme E2) degradation of DIAP1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
ERalpha is predominantly monoubiquitinated in a reaction that involves interactions with both BRCA1 and BARD1. Identification of ERalpha as a putative BRCA1/BARD1 ubiquitination substrate reveals a potential link between the loss of BRCA1/BARD1 ligase activity and tissue-specific carcinoma
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
protein ubiquitination plays an important role in the immune responses. This process is catalyzed by a cascade of enzymatic reactions, with the E3 ubiquitin ligases being the critical enzymes that determine the specificity of substrate recognition
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
Pseudomonas syringae injects the AvrPtoB type III effector protein into the plant cell to suppress programmed cell death associated with plant immunity. AvrPtoB manipulates the host ubiquitin machinery and employs intrinsic E3 Ub ligase activity to suppress hypersensitive response-based programmed cell death and plant immunity
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
PUB17 ligase activity is crucial for defense signaling. Arabidopsis PUB17 knockout plants are compromised in RPM1- and RPS4-mediated resistance against Pseudomonas syringae pv tomato containing avirulence genes AvrB and AvrRPS4, respectively
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
the breast and ovarian specific tumor suppressor BRCA1, together with BARD1, comprise an E3 ubiquitin ligase. BRCA1 effects on transcription and DNA damage repair can be mediated via the ubiquitin ligase activities directed at RNA polymerase II or topo IIalpha. The effects of BRAC1 on genomic stability in breast cells can be mediated by ubiquitination of centrosome proteins, including gamma-tubulin and Npm1
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
the ubiquitin ligase complex SCFFbs1 contributes to the ubiquitination of glycoproteins, is involved in the endoplasmic reticulum-associated degradation pathway
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
TRIM25 E3 ubiquitin ligase induces the Lys 63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
ubiquitin-protein E6AP is extensively involved in the ubiquitin mediated degradation of high-risk human papillomavirus E6-dependent substrates as a cellular E3 ubiquitin-protein ligase
-
-
?
ATP + ubiquitin + protein lysine
AMP + diphosphate + protein N-ubiquityllysine
show the reaction diagram
-
E3 mediates the ubiquitin transfer from E2, the ubiquitin-conjugating enzyme to the lysine residue(s) of the substrate
-
-
?
ATP + ubiquitin + protein p53
?
show the reaction diagram
Q00987
-
-
-
?
ATP + ubiquitin + PRP19 lysine
AMP + diphosphate + PRP19 N-ubiquityllysine
show the reaction diagram
-
auto ubiquitination
-
?
ATP + ubiquitin + synaptotagmin XI
AMP + diphosphate + synaptotagmin XI N-ubiquityllysine
show the reaction diagram
-
parkin binds to the C2A and C2B domains of synaptotagmin XI resulting in the polyubiquitination of synaptotagmin XI. The interaction with this protein suggests a role for parkin in the regulation of the synaptic vesicle pool and in vesicle release. Loss of parkin could affect multiple proteins controlling vesicle pools, docking and release and explain the deficits in dopaminergic function seen in patients with parkin mutations
-
-
?
ATP + ubiquitin + syntaxin 1 lysine
AMP + diphosphate + syntaxin 1 N-ubiquityllysine
show the reaction diagram
Q8CJB9
staring targets syntaxin 1 for proteasome dependent degradation
-
?
ATP + ubiquitin + ubiquitin carrier protein E2
AMP + diphosphate + ubiquitin-(ubiquitin carrier protein E2)
show the reaction diagram
-
-
-
-
?
ATP + ubiquitin + UFD2a lysine
AMP + diphosphate + UFD2a N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + UFD2b lysine
AMP + diphosphate + UFD2b N-ubiquityllysine
show the reaction diagram
O94941, Q9WUD1
auto ubiquitination
-
?
ATP + ubiquitin + UT-A1 urea transporter
?
show the reaction diagram
Q00987
-
-
-
?
ATP + ubiquitin + [epidermal growth factor receptor ErbB3]
AMP + diphosphate + [epidermal growth factor receptor ErbB3]-N-ubiquityllysine
show the reaction diagram
Q9H4P4
Nrdp1 may act to regulate steady-ste cell surface neuregulin receptor levels, thereby influencing the efficiency of neuregulin signaling
-
-
?
ATP + ubiquitin + [epidermal growth factor receptor ErbB3]
AMP + diphosphate + [epidermal growth factor receptor ErbB3]-N-ubiquityllysine
show the reaction diagram
-
Nrdp1 promotes degradation of Erb3 by proteasomes and thus, may be an important factor influencing cell growth
-
-
?
additional information
?
-
-
ability to enhance the ubiquitination and degradation of misfolded tubulins may play a significant role in protecting neurons from toxins that cause Parkinson‘s disease
-
-
-
additional information
?
-
Q9Y4X5
parkin functions as an E3 ubiquitin-protein ligase through its ring domains and may control protein levels via ubiquitination. The loss of Parkin‘s ubiquitin-protein ligase function in familial-linked mutations suggests that this may be the cause of familial autosomal recessive Parkinson‘s disease
-
-
-
additional information
?
-
-
each of the three PHD fingers of Msc1 can act as ubiquitin E3 ligases
-
-
-
additional information
?
-
-
Prp19 is an essential splicing factor and a member of the U-box family of E3 ubiquitin ligases
-
-
-
additional information
?
-
-
ataxia telangiectasia-mutated (ATM) and ataxia telangiectasia-mutated and Rad3-related (ATR) phosphorylate Siah-1 at Ser19 to modulate HIPK2-Siah-1 interaction
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Bmi1 performs self-ubiquitination
-
-
-
additional information
?
-
P38398
BRCA1 and BARD1 are required to recruit Rad51 and BRCA2, the central players in homologous recombination at damaged DNA sites, BRCA1-BARD1 in the Pol II-holoenzyme may act as a transcriptional co-activator
-
-
-
additional information
?
-
Q8WZ73
CARP-2 inhibits tumor necrosis factor-induced IkappaB kinase activation and IkappaBalpha degradation
-
-
-
additional information
?
-
-
Cbl-b associates with protein kinase C-theta upon T-cell antigen receptor stimulation and regulates T-cell antigen receptor-induced protein kinase C-theta activation via Vav-1, which couples protein kinase C-theta to phosphatidylinositol 3-kinase and allows it to be phosphorylated, the down-regulation of -cell antigen receptor-induced NF-kappaB activation by Cbl-b is mediated coordinately by both Akt-dependent and protein kinase C-theta-dependent signaling pathways in primary T cells
-
-
-
additional information
?
-
Q3TTA7
Clb-b plays a predominant role in peripheral T-cell activation and is critically involved in T-cell exhaustion and prevention of lethal disease at infection with murine lymphocytic choriomeningitis virus, Cbl-b ubiquitin ligase regulates virus-specific CD8+ T-cell expansion and antiviral function during acute and chronic infections
-
-
-
additional information
?
-
Q9H992
deubiquitylation is responsible for the USP9Xand USP7-mediated stabilization of MARCH7
-
-
-
additional information
?
-
Q6ZNA4, Q96J02, Q96PU5
E3 ubiquitin ligases catalyze the ubiquitination of both their substrates and themselves, in many cases, E3 autoubiquitination induces their proteasome-dependent degradation, suggesting that autoubiquitination controls E3 ubiquitin ligase abundance
-
-
-
additional information
?
-
Q5RJY2
G2E3 is capable of autoubiquitination
-
-
-
additional information
?
-
Q7Z6Z7
HUWE1 is implicated in TP53-associated regulation of the neuronal cell cycle
-
-
-
additional information
?
-
-
interaction of human immunodeficiency virus type 1 Vif with the cullin 5 ubiquitin ligase is required for cell cycle disruption
-
-
-
additional information
?
-
Q9H992
MARCH7 readily undergoes autoubiquitylation
-
-
-
additional information
?
-
Q00987
MDM2 is capable of autoubiquitination and degradation via the proteasome
-
-
-
additional information
?
-
P46934
Nedd4 inhibits PTEN-induced apoptotic cell death
-
-
-
additional information
?
-
-
Pam F3 is ubiquitinated when co-transfected with Ub-HA in HEK293T cells
-
-
-
additional information
?
-
Q13618
potassium channel tetramerization domain 5 is a substrate-specific adaptor for cullin3-based E3 ligases
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Rae28 impairs ubiquitin-proteasome-mediated degradation of geminin and increases protein stability, Rae28 mediates recruiting Scmh1, which provides PcG complex 1 an interaction domain for geminin
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Rae28 performs self-ubiquitination
-
-
-
additional information
?
-
Q68DV7
recombinant RNF43 fused with maltose-binding protein has autoubiquitylation activity
-
-
-
additional information
?
-
Q3U827
Rines is heavily ubiquitinated and degraded by proteasome
-
-
-
additional information
?
-
P35226, Q64028, Q9CQJ4
Ring1B performs self-ubiquitination
-
-
-
additional information
?
-
Q9P0P0
RN181 is a ubiquitin E3 ligase which is self-ubiquitinated as part of the enzymatic reaction, RN181 plays a role for ubiquitination in integrin and platelet signalling
-
-
-
additional information
?
-
O88846
RNF4 undergoes auto-ubiquitination, RNF4 preferentially binds poly-SUMO-2 chains with relatively weak binding to mono- or di-SUMO
-
-
-
additional information
?
-
-
Siah1 does not promote P-glycoprotein degradation but decreases its expression transcriptionally by promoting c-Jun transcription factor binding to the activator protein 1 (AP1) site in the MDR1 promoter, blocking proteasome function by MG132 does not prevent the downregulation of P-glycoprotein by Siah1, Siah1 triggers c-Jun NH2-terminal kinase activation, leading to enhanced phosphorylation of c-Jun, and the JNK/c-Jun signalling axis is critical for Siah1 to down-regulate MDR1/P-glycoprotein expression
-
-
-
additional information
?
-
-
Skp1a is a cellular interaction partner of the 68k ankyrin-like protein from orthopoxviruses, SCF complex formation is likely to have a functional role during orthopoxviral infections
-
-
-
additional information
?
-
Q96845
the active-site cysteine of the HECT domain is critical for association of Nedd4-2s with virus-like particles
-
-
-
additional information
?
-
-
the CUL4-DDB1 ubiquitin ligase interacts with Raptor and regulates the mTORC1-mediated signaling pathway through ubiquitin-dependent proteolysis
-
-
-
additional information
?
-
-
the E3 ligase is required to trigger the metaphase to anaphase transition by degrading the separase inhibitor securin
-
-
-
additional information
?
-
-
the herpes simplex virus UL56 protein is not ubiquitinated despite its interaction with Nedd4
-
-
-
additional information
?
-
-
the poxvirus Orf virus use ankyrin repeat protein 008 to dictate target specificity to SCF1 ubiquitin ligase and thereby exploit the cell's ubiquitin-proteasome machinery, the protein interacts with the SCF1 complex component SKP1 in an F-box-dependent but ankyrin-independent manner
-
-
-
additional information
?
-
Q9C035
TRIM5alpha does not ubiquitinate Ro52, TRIM5alpha ubiquitinates itself and is also ubiquitinated by the E3 ubiquitin ligase Ro52, is deubiquitinated by YopJ (one of the pathogenic proteins derived from Yersinia species), and selectively deubiquitinated by UnpEL, the ubiquitination of TRIM5a does not lead to its proteasomal degradation in HeLa cells
-
-
-
additional information
?
-
-
VprBP strongly associates with DDB1-CUL4 and binds to chromatin and functions by either recruiting specific substrates or promoting the recruitment of other substrates to the DDB1-CUL4-ROC1 E3 ligase
-
-
-
additional information
?
-
-
E6-AP induces ubiquitination and degradation of misfolded luciferases that are bound with Hsp70
-
-
-
additional information
?
-
-
Fbw7, the substrate recognition subunit of SCFFbw7 ubiquitin ligase, facilitates the degradation of several proto-oncogene products by the proteasome
-
-
-
additional information
?
-
Q96PM5
isozymes Pirh2B and Pirh2C can downregulate p53 protein levels and promote intracellular p53 ubiquitination
-
-
-
additional information
?
-
-
recombinant REDD1 is rapidly degraded by the ubiquitin-proteasome system mediated by the CUL4A-DDB1-ROC1-beta-TRCP E3 ligase complex, i.e. cullin 4A-DNA damage-binding protein 1-regulator of cullins 1-ubiquitin ligase complex, through glycogen synthase kinase 3-beta phosphorylation and beta-transducin repeat-containing protein activity, overview
-
-
-
additional information
?
-
-
recombinant Triad1 inhibits clonogenic growth and proliferation of transfected U937 cells, requiring the RING fingers, without affecting their differentiation and apoptosis
-
-
-
additional information
?
-
-
Rsp5p inhibits Tomato bushy stunt virus, TBSV, replication in recombinant Saccharomyces cerevisiae by direct binding between Rsp5p and p92pol reducing the stability of p92pol, interaction analysis, overview. The WW domain of Rsp5p, which is involved in protein interactions, is responsible for inhibition of TBSV replication, whereas the HECT domain, involved in protein ubiquitination, is not necessary for Rsp5p-mediated inhibition of viral replication
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ubiqitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E1
-
-
-
ubiquitin-conjugating enzyme E2
Q9CR50
absolutely required for activity
-
ubiquitin-conjugating enzyme E2
Q14669
UbcH5a
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
CHIP interacts preferentially with two types of E2 enzymes, UbcH5 and Ubc13-Uev1a, CHIP also interacts productively with the class III E2 enzyme Ube2e2, CHIP undergoes auto-ubiquitination by UbcH5 but not by Ubc13-Uev1a, CHIP drives the formation of unanchored polyubiquitin by Ubc13-Uev1a, the E2 enzymes UbcH7 and UbcH7-K96S do not carry out polyubiquitination with CHIP
-
ubiquitin-conjugating enzyme E2
Q92462, Q94TG2
;
-
ubiquitin-conjugating enzyme E2
P38398
the RING finger domain of BRCA1 is capable of interacting with E2 enzymes UbcH6, UbcH7, Ube2e2, UbcM2, Ube2w, Ubc13, and Ube2k in addition to UbcH5
-
ubiquitin-conjugating enzyme E2
-
Pam F3 is able to bind specifically to UbcH5a, UbcH5c, and UbcH7
-
ubiquitin-conjugating enzyme E2
-
the E2 Ub-conjugating enzyme transfers the activated ubiquitin to the targeted protein
-
ubiquitin-conjugating enzyme E2
Q5D267, Q99F15, Q9SVC6
UbcH5b; UbcH5b; UbcH5b
-
ubiquitin-conjugating enzyme E2
Q86XT4
direct interaction with ubiquitin-conjugating enzyme E2, preferential binding with UbcH8, strong binding with UbcH6 and UbcH9, weak binding with UbcH5, no binding with UbcH2
-
ubiquitin-conjugating enzyme E2
Q810I2
direct interaction with ubiquitin-conjugating enzyme E2, preferential binding with UbcH8, strong binding with UbcH6 and UbcH9, weak binding with UbcH5, no binding with UbcH2
-
ubiquitin-conjugating enzyme E2
Q68DV7
polyubiquitylation is detected only with UbcH5b or UbcH5c as an E2 source
-
ubiquitin-conjugating enzyme E2
Q9C035
Ro52 ubiquitinates itself in cooperation with the E2 ubiquitin-conjugating enzyme UbcH5B and not by other E2 enzymes; TRIM5alpha ubiquitinates itself in cooperation with the E2 ubiquitin-conjugating enzyme UbcH5B and not by other E2 enzymes
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
Q3U827
UbcH6
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
RBCK1 cooperates with UbcH7 as an E2
-
ubiquitin-conjugating enzyme E2
Q5RJY2
G2E3 is very active with E2 enzyme UbcH5a, very little or no activity is observed with E2 enzymes UbcH1, UbcH2, UbcH3, UbcH7, UbcH9, or UbcH10
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
-
MDM2 prefers UbcH5B
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
P90495
;
-
ubiquitin-conjugating enzyme E2
-
Cdc34p E2 ubiquitin-conjugating enzyme
-
ubiquitin-conjugating enzyme E2
-
uses Cdc34 or UbcH5c as E2
-
ubiquitin-conjugating enzyme E2
Q5ZRQ0
LubX has ubiquitin ligase activity in conjunction with UbcH5a or UbcH5c E2 enzymes, the U-box 1 of LubX serves as the E2 binding site
-
ubiquitin-conjugating enzyme E2
O88846
ubiquitination of SUMO-2 polymers by RNF4 is most efficient in the presence of either Ubc4 or one of the UbcH5 isoforms
-
ubiquitin-conjugating enzyme E2
-
UbcH5
-
ubiquitin-conjugating enzyme E2
A2A5Z6
UbcH5c; UbcH5c
-
ubiquitin-conjugating enzyme E2
-
UBE2D2 (UbcH5b)
-
ubiquitin-conjugating enzyme E2
Q84TG3, Q9SVC6
UBC8; UBC8
-
ubiquitin-conjugating enzyme E2
-
ORTH1 shows robust activity with UBC8, UBC10 and UBC11, ORTH1 also shows diminished activity with UBC28 and UBC29, ORTH1 is not active with UBC34, UBC27, UBC30, UBC32, and UBC36
-
ubiquitin-conjugating enzyme E2
-
-
-
ubiquitin-conjugating enzyme E2
Q969V5
Ubc4
-
ubiquitin-conjugating enzyme E2
-
Ubc4
-
ubiquitin-conjugating enzyme E2
-
UbcH5b
-
ubiquitin-conjugating enzyme E2
-
TRIM2 uses UbcH5a as ubiquitin-conjugating enzyme E2
-
ubiquitin-conjugating enzyme E2
-
HLTF interacts with the Rad18 and Mms2-Ubc13 ubiquitin-conjugating enzyme complexes
-
ubiquitin-conjugating enzyme E2
Q9H992
wild type MARCH7 binds two E2-conjugating enzymes, Huntingtin-interacting protein 2 and Ubc13
-
ubiquitin-conjugating enzyme E2
-
Ubc4 promotes rapid mono-ubiquitination of multiple lysines on APC/C targets, whereas Ubc1 catalyzes K48-linked polyubiquitin chain assembly on preattached ubiquitins
-
ubiquitin-conjugating enzyme E2
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
Ca2+ activates Nedd4 E3 ubiquitin ligase by releasing the C2 domain-mediated auto-inhibition in both isoform Nedd4-1 and Nedd4-2. Maximal activation of Nedd4-2a is achieved by 0.001 mM Ca2+
Mg2+
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
adenosyl-phospho-ubiquitinol
-
competitive to ATP, noncompetitive to ubiquitin
-
Akt
-
inhibits Ku70 proteolysis in the cytosol
-
aurora A kinase
-
phosphorylates BRCA1 resulting in diminished ubiquitin ligase activity
-
carboplatin
-
weakest inhibitor, partial E3 ligase activity is still observed for the carboplatin-BRCA1 adducts at concentrations that exceed 1 mM
cisplatin
-
complete inhibition at 0.1 mM
E1A
-
an oncogene product derived from adenovirus, interferes with the activity of the SCFFbw7 ubiquitin ligase. E1A inhibits the ubiquitin ligase activity of the Roc1/Rbx1-CUL1 complex but not that of another RING-type ubiquitin ligase, Mdm2. E1A directly targets Roc1 and CUL1, inhibition mechanism and binding to subunit Fbw7, overview
-
ghrelin
-
inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx
-
KVGFFKR
Q9P0P0
the interaction between RN181 and platelet integrin alphaIIbbeta3 is competitively inhibited in a dose-dependent manner using a synthetic KVGFFKR peptide (0.01-0.1 mM)
lithium chloride
Q94297
prevents the UV-induced securin downregulation by SKP1-CUL1-beta TrCP
LY294002
P46934
Nedd4 expression is decreased by the phosphoinositide 3-kinase inhibitor LY294002
nutlin-3
Q00987
-
nutlin-3
-
inhibits and/or disrupts the Ku70-Hdm2 interaction
oxaliplatin
-
complete inhibition at 0.25 mM
protein kinase Cbeta
-
RBCK1 undergoes efficient phosphorylation by PKCbeta, the phosphorylated RBCK1 shows no self-ubiquitination activity in vitro
-
PTEN
P46934
the Nedd4 level is down-regulated by PTEN (phosphatase and tensin homologue deleted on chromosome 10), Nedd4 is transcriptionally regulated by PTEN
-
RBCK2
-
RBCK1 is inhibited by interaction with its splice variant RBCK2
-
SCF-I2
-
inhibits the binding and ubiquitination of full-length phosphorylated substrates by SCFCdc4 ubiquitin ligase
sempervirine
-
inhibits MDM2 auto-ubiquitination and MDM2-mediated p53 degradation
TDZD-8
Q94297
prevents the UV-induced securin degradation by SKP1-CUL1-beta TrCP
transplatin
-
strongest inhibitor, complete inhibition at 0.08 mM
-
UL56
-
the herpes simplex virus UL56 protein interacts with the ubiquitin ligase Nedd4 and increases its ubiquitination, Nedd4 is phosphorylated and degraded in wild type HSV-2-infected cells but not in cells infected with a UL56-deficient mutant, UL56 also causes a decrease in Nedd4 protein levels
-
vascular endothelial growth factor
-
causes Hdm2 nuclear translocation and thereby inhibiting Ku70 protein degradation
-
MG-132
-
0.01 mM effectively blocks degradation of p27
additional information
-
MAFbx protein level declines as insulin increases, with MuRF-1 expression is largely unchanged
-
additional information
-
phosphorylation of Nedd4-2 by Sgk1 regulates its binding to epithelial Na+ channel subunits and thus ubiquitination
-
additional information
-
not inhibited by etidronate and pamidronate
-
additional information
-
expression of Fbw7alpha is down-regulated by UVC or UVB, independently of the p53, MAPK and the PKC pathways but the repression is inhibited in the absence of active Fbw7 proteins
-
additional information
Q91Z63
growth hormone releasing peptide-2 decreases MuRF1 mRNA level irrespective in the presence of dexamethasone
-
additional information
-
in response to DNA damage, checkpoint kinase ATM and/or ATR is activated and mediates homeodomain-interacting protein kinase 2 stabilization through disruption of the homeodomain-interacting protein kinase 2-Siah-1 complex by phosphorylating Siah-1 at Ser19
-
additional information
-
Orf virus ankyrin repeat protein 008 does not inhibit the activity of its associated E3 ligase
-
additional information
-
the Huwe1-N-myc pathway is targeted by genetic and epigenetic alterations in malignant brain tumors
-
additional information
-
at higher Triad1 concentrations, autoinhibition of the ubiquitination reactions occurs
-
additional information
-
contrary to the Escherichia coli enzyme, no inhibition of MurFSa by UDPMurNAc-tripeptide is observed
-
additional information
-
the C2 domain is auto-inhibitory for Nedd4 E3 ubiquitin ligases
-
additional information
-
SCF-I2 fails to inhibit Cdc4 activity in vivo
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
BARD1
P38398
BARD1 interaction is required to stabilize the proper conformation of the BRCA1 RING domain for E3 activity
-
bufalin
-
5 nM bufalin leads to up-regulation of the Cbl family of ubiquitin ligases, CsA (an inhibitor of calcium mobilization) reverses this up-regulation, the up-regulation of Cbl proteins does not affect cell differentiation, but may be involved in cell adhesion
c-Jun NH2-terminal kinase
-
-
-
Carbachol
-
-
casein kinase-2 interacting protein-1
A2A5Z6
Smurf1 interacts with casein kinase-2 interacting protein-1 resulting in an increase in its E3 ligase activity
-
cdc34
-
supports ubiquitination of IkappaBalpha in vitro
-
cdc34
-
E2 ubiquitin-conjugating enzyme
-
dexamethasone
Q91Z63
0.01 mM dexamethasone increases both atrogin-1 and MuRF1 mRNA levels, the increased mRNA level of atrogin-1 is significantly attenuated by growth hormone releasing peptide-2
Doxorubicin
-
atrogin-1 mRNA is upregulated with 15 mg/kg doxorubicin treatment through a p38-MAPK-dependent pathway
Doxorubicin
-
atrogin-1 mRNA is upregulated with 0.0001 mg doxorubicin treatment through a p38-MAPK-dependent pathway
E1B55K
-
E1B55K associates with the E4orf6 protein and binds to specific substrate proteins to bring them to the complex for ubiquitination
-
E4orf6
-
highjacks a cellular Cul5-based E3 ligase in order to ubiquitinate and degrade specific substrates brought to the complex by E1B55K
-
Epidermal growth factor
-
-
fibroblast growth factor 2
-
expression is induced by cell treatment with 10 ng/ml FG-2
-
Flp1p
Q92462, Q94TG2
activates a rapid Pub1p-independent degradation of Cdc25p at the end of the cell cycle to coordinate mitosis with cytokinesis; Flp1p interacts in vivo with Pub2p strongly suggesting that Flp1 may also regulate Pub1/2 activity
-
H2O2
-
0.5 mM H2O2 stimulates SCFbeta-TrCP ubiquitin ligase-mediated ubiquitination of RCAN1
hDET1
-
essential for the degradation of c-Jun by Cop1
-
high-risk human papillomavirus E6 protein
-
hScrib is targeted for E6AP dependent multiubiquitination in the presence of high-risk HPV E6
-
NEDD8
-
covalent attachment of the ubiquitin-like modifier Nedd8 to cullin proteins (neddylation) activates SCF complexes
-
OsUBC5a
Q8S919
ubiquitin-conjugating enzyme E2
-
OsUBC5b
Q8S919
ubiquitin-conjugating enzyme E2
-
Polo-like kinase 2
-
in the presence of Polo-like kinase 2, spine-associated Rap GTPase activating protein physically associates with the SCFbeta-TRCP complex through a canonical phosphodegron
-
Proliferating cell nuclear antigen
-
proliferating cell nuclear antigen promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4Cdt2 E3 ubiquitin ligase complex and is required for the efficient ubiquitylation and degradation of p21
-
Rbx1
-
Rbx1 is an essential component of most SCF complexes and mediates the interaction with the E2 enzyme
-
Smad2
Q6ZNA4, Q96PU5
in response to TGF-beta ligation, Smad2 forms a complex with Smurf2, which mediates the recruitment of Smurf2 to its substrate, the transcriptional corepressor SnoN
-
suppressor of cytokine signalling 3
Q9D5V5
suppressor of cytokine signalling 3 (SOCS3) can associate with functional E3 ubiquitin ligases and thereby induce the degradation of bound signalling proteins, the SOCS box acts as an independent binding domain capable of recruiting elonginBC and cullin5 to promote E3 ligase formation
-
TNFalpha
-
the E3 ligase activity of Mule is stimulated by TNFalpha
-
tumor necrosis factor
-
tumor necrosis factor treatment (7.5 ng/ml) induces the expression of ubiquitin ligase Smurf1 in chronic inflammatory disorders
-
Ubc2b
-
E2 ubiquitin conjugating enzyme
-
Ubc3
-
E2 ubiquitin conjugating enzyme
-
UBC4
O94941, Q9WUD1
E2 ubiquitin conjugating enzyme
-
UBC4
-
supports ubiquitination of IkappaBalpha in vitro
-
UBC4
-
ubiqitin conjugating enzyme E2
-
Ubc5
-
supports ubiquitination of IkappaBalpha in vitro
-
UbcH5
-
E2 ubiquitin-conjugating enzyme
-
UbcH5B
-
C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes, i.e. E2s
-
UbcH5C
O94941, Q9WUD1
E2 ubiquitin conjugating enzyme
-
UbcH6
-
C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes, i.e. E2s
-
UbcH7
-
E2 ubiquiting conjugating enzyme
-
UbcH7
-
E2 ubiquitin conjugating enzyme
-
UbcH7
-
ubiquitin-conjugating enzyme
-
UbcH8
-
E2 ubiquiting conjugating enzyme
-
UbcH8
Q8CJB9
E2 ubiquitin-conjugating enzyme
-
UbcH8
Q86XT4
Trim50 expression is increased in the presence of UbcH8 (also namend UBE2L6)
-
UbcH8
Q810I2
Trim50 expression is increased in the presence of UbcH8 (also namend UBE2L6)
-
UbcH9
-
C4C4 RING domain of CNOT4 interacts with a subset of ubiquitin-conjugating enzymes, i.e. E2s
-
UBXD7
-
UBXD7 links p97 to the ubiquitin ligase CUL2/VHL and its substrate hypoxia-inducible factor 1alpha
-
USP7
Q9H992
MARCH7 is stabilized by USP9X which deubiquitylates MARCH7 in the nucleus
-
USP9X
Q9H992
MARCH7 is stabilized by USP9X which deubiquitylates MARCH7 in the cytosol
-
virion infectivity factor
-
the HIV virion infectivity factor causes the proteasome-mediated destruction of human antiviral protein APOBEC3G by tethering it to a cellular E3 ubiquitin ligase, HIV virion infectivity factor hijacks the human E3 ligase through two regions within its C-terminal domain: a BC box region that interacts with elonginC and a novel zinc finger motif that interacts with cullin5
-
MmUBC7
-
E2 ubiquitin-conjugating enzyme
-
additional information
Q5D267, Q99F15, Q9SVC6
expression of PUB22 is induced by pathogen-associated molecular pattern flg22 and infection by pathogens; expression of PUB23 is induced by pathogen-associated molecular pattern flg22 and infection by pathogens; expression of PUB24 is induced by pathogen-associated molecular pattern flg22 and infection by pathogens
-
additional information
Q94297
UV-radiation induces degradation of securin by SKP1-CUL1-beta TrCP in both the nucleus and cytoplasm
-
additional information
-
neddylation with Nedd8 enhances Cdc34 binding to SCF and enhances both E2 recruitment and beta-catenin turnover
-
additional information
-
OS-9 and XTP3-B/Erlectin are endoplasmic reticulum-resident glycoproteins that bind to endoplasmic reticulum-associated degradation substrates and, through the SEL1L adaptor, to the endoplasmic reticulum-membrane-embedded ubiquitin ligase Hrd1, the proteins are components of distinct, partially redundant, quality control surveillance pathways that coordinate protein folding with membrane dislocation and ubiquitin conjugation
-
additional information
Q16531
VprBP/DCAF1 is a putative substrate adaptor for cullin 4-based E3 ubiquitin ligase
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.11
ATP
-
pH 8.6, 37°C
0.0011
beta-catenin lysine
-
neddylated SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0024
beta-catenin lysine
-
neddylated SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.012
beta-catenin lysine
-
SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.017
beta-catenin lysine
-
SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.00086
beta-catenin N-ubiquityllysine
-
neddylated SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.00092
beta-catenin N-ubiquityllysine
-
neddylated SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0023
beta-catenin N-ubiquityllysine
-
SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0028
beta-catenin N-ubiquityllysine
-
SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.046
D-Ala-D-Ala
-
pH 8.6, 37°C
0.0026
p27 lysine
-
neddylated SCFSkp2, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.003
p27 lysine
-
neddylated SCFSkp2, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0061
p27 lysine
-
SCFSkp2, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0091
p27 lysine
-
SCFSkp2, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.046
UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys
-
pH 8.6, 37°C
0.36
methylenebisphosphonate
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Vmax: 71 micromol/min/mg, pH 8.6, 37°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0009
beta-catenin lysine
-
SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0032
beta-catenin lysine
-
neddylated SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0155
beta-catenin lysine
-
SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0383
beta-catenin lysine
-
neddylated SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0025
beta-catenin N-ubiquityllysine
-
SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.03
beta-catenin N-ubiquityllysine
-
SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0383
beta-catenin N-ubiquityllysine
-
neddylated SCFbeta-TrCP, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0467
beta-catenin N-ubiquityllysine
-
neddylated SCFbeta-TrCP, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.00467
p27 lysine
-
neddylated SCFSkp2, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0047
p27 lysine
-
SCFSkp2, using Cdc34 as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0075
p27 lysine
-
SCFSkp2, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.0183
p27 lysine
-
neddylated SCFSkp2, using UbcH5c as ubiquitin-conjugating enzyme E2, in 50 mM Tris-HCl (pH 7.5), 60 mM NaCl, 10 mM MgCl2, and 1 mM dithiothreitol, at 30°C
-
0.15
methylenebisphosphonate
-
-
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.78
carboplatin
-
in 50 mM Tris (pH 7.5), 0.5 mM dithiothreitol, 5 mM ATP, 2.5 mM MgCl2, and 0.005 mM ZnCl2, at 37°C
0.06
cisplatin
-
in 50 mM Tris (pH 7.5), 0.5 mM dithiothreitol, 5 mM ATP, 2.5 mM MgCl2, and 0.005 mM ZnCl2, at 37°C
0.15
oxaliplatin
-
in 50 mM Tris (pH 7.5), 0.5 mM dithiothreitol, 5 mM ATP, 2.5 mM MgCl2, and 0.005 mM ZnCl2, at 37°C
0.0019
SCF-I2
-
pH and temperature not specified in the publication
0.053
transplatin
-
in 50 mM Tris (pH 7.5), 0.5 mM dithiothreitol, 5 mM ATP, 2.5 mM MgCl2, and 0.005 mM ZnCl2, at 37°C
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.258
-
-
58
-
pH 8.6, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.4
-
assay at
7.5
-
assay at
7.5
-
assay at
8
-
assay at
8.6
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
assay at
30
P35131
assay at
37
-
assay at
43
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.13
Q8CJB9
predicted from nucleotide sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
P35131
RMA1 and RMA3
Manually annotated by BRENDA team
Q3U827
in the adult brain and in the developing brain particularly in the ventricular layer of the cerebral cortex at embryonic stages
Manually annotated by BRENDA team
-
the pattern of expression of the Huwe1 protein in the nervous system shows an increasing gradient, with the lowest amounts in the regions that contain neural stem cells/undifferentiated progenitors and the highest levels in differentiated neurons, expression through development, overview
Manually annotated by BRENDA team
Q5RJY2
predominantly expressed within the central nervous system
Manually annotated by BRENDA team
-
expressed in Purkinje cells of the cerebellar cortex
Manually annotated by BRENDA team
-
in the cerebellum, the strongest expression is in Purkinje cells and in the deep cerebellar nuclei
Manually annotated by BRENDA team
P35131
high expression of RMA1 and RMA3
Manually annotated by BRENDA team
Q9H992
MARCH7 is highly expressed in immune, neuronal and embryonic stem cells
Manually annotated by BRENDA team
P35131
high expression of RMA1 and RMA3
Manually annotated by BRENDA team
Q969V5
highly expressed in heart
Manually annotated by BRENDA team
-
CD34+ umbilical cord blood cell
Manually annotated by BRENDA team
-
a low level of expression of Pirh2 is found both at transcriptional and translational level in human hepatocellular carcinoma
Manually annotated by BRENDA team
-
in pyramidal cells of CA1-CA3 hippocampal areas and in granule cells of the dentate gyrus
Manually annotated by BRENDA team
P35131
RMA2 expression is restricted to the root tips and leaf hydathodes
Manually annotated by BRENDA team
Q3U827
developing lens
Manually annotated by BRENDA team
-
MURF1 shows strong postnatal up-regulation, MURF2 is expressed at the very onset of mouse cardiac differentiation at embryonic day 8.5, and represents a sensitive marker for differentiating myocardium. During cardiac development, expression shifts from the 50 kDa to the 60 kDa A-isoform which dominates postnatally, MURF3 protein expression is negligible during embryonic cardiac development and only upregulated postnatally
Manually annotated by BRENDA team
-
Fbxo45 is selectively expressed in the nervous system
Manually annotated by BRENDA team
Q76N89
highly expressed in favorable neuroblastomas as compared with unfavorable ones
Manually annotated by BRENDA team
-
of hippocampus
Manually annotated by BRENDA team
-
peripheral sensory neuron
Manually annotated by BRENDA team
-
overexpressed in serous ovarian carcinoma
Manually annotated by BRENDA team
-
HRD1 is expressed widely in the substantia nigra pars compacta containing dopaminergic neurons
Manually annotated by BRENDA team
P35131
RMA1 and RMA3: shoot-root junction and roots
Manually annotated by BRENDA team
P35131
RMA2 expression is restricted to the root tips and leaf hydathodes
Manually annotated by BRENDA team
P35131
RMA1 and RMA3: shoot-root junction
Manually annotated by BRENDA team
-
localized in the sperm head and tail
Manually annotated by BRENDA team
Q8C863
CD4+CD25+ and CD4+CD25- T-lymphocytes
Manually annotated by BRENDA team
-
ectopic expression of AIP2 in mouse primary T-cells enhances their proliferation and interleukin-2 production by suppressing the apoptosis of T-cells
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
ectopic expression of AIP2 in mouse primary T-cells enhances their proliferation and interleukin-2 production by suppressing the apoptosis of T-cells
-
Manually annotated by BRENDA team
-
expression of MEX is restricted to testis
Manually annotated by BRENDA team
-
the enzyme is exclusively expressed in the testis, mainly in the germ cells during the late stage of spermatogenesis
Manually annotated by BRENDA team
additional information
Q05086
E6-AP is expressed in all human tissues
Manually annotated by BRENDA team
additional information
-
HLTF is frequently inactivated in colorectal and gastric cancers
Manually annotated by BRENDA team
additional information
-
HRD1 is not detected in glial cells
Manually annotated by BRENDA team
additional information
-
not detected in brain, kidney, and liver
Manually annotated by BRENDA team
additional information
P35131
AtRMAs are differentially expressed in various tissues, overview
Manually annotated by BRENDA team
additional information
-
Fbox45 mRNA is undetectable in the tissues outside the nervous system
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme is associated with all three components of the cytoskeleton: actin filaments, tubulin filaments, intermediate filaments
Manually annotated by BRENDA team
-
BRCA1 and BARD1 localize to centrosomes throughout the cell cycle
Manually annotated by BRENDA team
-
localization may be regulated in a cell cycle-dependent manner
Manually annotated by BRENDA team
O94941, Q9WUD1
localization might be regulated in a cell cycle-dependent manner
Manually annotated by BRENDA team
-
isoform Fbw7beta
Manually annotated by BRENDA team
Q9C035
monoubiquitination is a signal for Ro52 to translocate from cytoplasmic bodies to the cytoplasm, Ro52 is diffusely located in the cytoplasm, monoubiquitination is a signal for TRIM5alpha to translocate from cytoplasmic bodies to the cytoplasm, TRIM5alpha is diffusely located in the cytoplasm
Manually annotated by BRENDA team
Q5RJY2
the enzyme shuttles between the cytoplasm and nucleus, concentrating in nucleoli and relocalizing to the nucleoplasm in response to DNA damage
Manually annotated by BRENDA team
-
apoptotic stress induces Hdm2 nuclear translocation
Manually annotated by BRENDA team
-
mostly cytosolic in healthy cells
Manually annotated by BRENDA team
-
HRD1 plays an important role in endoplasmic reticulum-associated degradation
Manually annotated by BRENDA team
-
Myc-tagged wild type Kf-1 displays a reticular pattern typical of endoplasmic reticulum localization with large perinuclear aggregates
Manually annotated by BRENDA team
P35131
RMA proteins contain a RING motif and a transmembrane domain in their N-terminal and extreme C-terminal regions, respectively
Manually annotated by BRENDA team
-
enzyme is associated with all three components of the cytoskeleton: actin filaments, tubulin filaments, intermediate filaments
Manually annotated by BRENDA team
-
tightly bound to
Manually annotated by BRENDA team
-
two transmembrane domains mediate MULAN's localization to the mitochondrion's outer membrane, MULAN is oriented such that its E3-active, C-terminal RING finger is exposed to the cytosol
Manually annotated by BRENDA team
Q969V5
two transmembrane domains mediate MULAN's localization to the mitochondrion's outer membrane, MULAN is oriented such that its E3-active, C-terminal RING finger is exposed to the cytosol
Manually annotated by BRENDA team
-
isoform Fbw7alpha
Manually annotated by BRENDA team
-
localization may be regulated in a cell cycle-dependent manner
Manually annotated by BRENDA team
O94941, Q9WUD1
localization might be regulated in a cell cycle-dependent manner
Manually annotated by BRENDA team
Q68DV7
nuclear envelope
Manually annotated by BRENDA team
-
isoform Fbw7gamma
Manually annotated by BRENDA team
-
ORTH1 and ORTH2 are localized to the nucleus
Manually annotated by BRENDA team
-
Skp1, Skp2, and Cul1
Manually annotated by BRENDA team
Q5RJY2
the enzyme shuttles between the cytoplasm and nucleus, concentrating in nucleoli and relocalizing to the nucleoplasm in response to DNA damage
Manually annotated by BRENDA team
-
apoptotic stress induces Hdm2 nuclear translocation
Manually annotated by BRENDA team
O60291
MGRN1 isoforms containing exon 12 are targeted to the nucleus by the MCRs
Manually annotated by BRENDA team
-
enzyme is associated with all three components of the cytoskeleton: actin filaments, tubulin filaments, intermediate filaments
-
Manually annotated by BRENDA team
-
although mostly cytosolic in healthy cells, upon induction of apoptosis, IBRDC2 accumulates in mitochondrial domains enriched with Bax. Both mitochondrial localization and apoptotic activation of Bax are required for IBRDC2 translocation to the mitochondria
Manually annotated by BRENDA team
additional information
Q9UTG2
Pub2-GFP fusion protein is found throughout the cell, especially at the cell surface
-
Manually annotated by BRENDA team
additional information
Q9C035
recombinant Ro52 is hardly detectable in the nucleus, recombinant TRIM5alpha is hardly detectable in the nucleus
-
Manually annotated by BRENDA team
additional information
O60291
agonist-induced internalization of wild-type andmutant MGRN1 isozymes, overview
-
Manually annotated by BRENDA team
additional information
-
E6-AP redistributes to microtubule-organizing center in response to proteasomal Inhibition
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Plasmodium vivax (strain Salvador I)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36000
Q9P0P0
SDS-PAGE and non-reducing PAGE
690719
50000
-
-
714798
60000
-
-
714798
66000
-
FLAG-tagged isoform Fbw7gamma, SDS-PAGE
693304
70000
-
-
714798
75000
-
recombinant enzyme fused to the maltose binding protein, SDS-PAGE
690923
90000
Q68DV7
SDS-PAGE
692211
97110
Q5T447
calculated from amino acid sequence
690715
100000
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0
-
691588
110000
-
SDS-PAGE
693906
115000
-
nondenaturing PAGE
1107
115000
-
gel filtration
1116
115000
-
SDS-PAGE
693120
121300
Q9M0V3
calculated from amino acid sequence
694630
127000
Q16531
calculated from amino acid sequence
694832
130000
-
SDS-PAGE
690380
179600
Q76N89
calculated from amino acid sequence
694490
210000
-
gel filtration
1103
220000
P38398
SDS-PAGE
691681
220400
Q14669
calculated from amino acid sequence
690743
224800
P19812
calculated from amino acid sequence
693121
250000
Q86XT4
gel filtration
692167
250000
Q810I2
gel filtration
692167
481900
-
calculated from amino acid sequence
694304
500000
O95071, P46934, Q05086, Q7Z6Z7, Q96J02, Q9H0M0
-
691588
500000
-
-
703367
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
Q68DV7
x * 90000, SDS-PAGE
?
-
x * 114291, calculation from nucleotide sequence, x * 117715, calculation from nucleotide sequence
?
-
x * 40000, SDS-PAGE
?
-
x * 30000, SDS-PAGE
?
-
x * 115000, SDS-PAGE
?
Q8CJB9
x * 113800, deduced from nucleotide sequence, x * 125000, SDS-PAGE, immunoblot
?
-
x * 300000, SDS-PAGE, immunoblot
?
Q9UTG2
x * 77000, deduced from nucleotide sequence
?
Q9LQ92
x * 35392, calculated from amino acid sequence
?
Q9H992
x * 78051, calculated from amino acid sequence
?
-
x * 26400, calculated from amino acid sequence
?
-
x * 75000, GST-tagged enzyme, SDS-PAGE
dimer
-
2 * 105000, SDS-PAGE
dimer
Q13191
Cbl-b dimerization is regulated by ubiquitin binding and required for tyrosine phosphorylation of Cbl-b and ubiquitination of Cbl-b substrates
heterodimer
P38398
-
homodimer
-
x-ray crystallography
homodimer
P35226, Q64028, Q9CQJ4
2 * 106317, calculated from amino acid sequence
homodimer
Q9P0P0
2 * 18000, non-reducing PAGE
homodimer
-
2 * 31123, calculated from amino acid sequence
homodimer
O43791
2 * 42132, calculated from amino acid sequence
homooligomer
Q969V5
x * 39800, calculated from amino acid sequence
monomer
-
117000-126000, SDS-PAGE 3 structurally related enzyme forms, MW 117000, MW 123000, MW 126000
trimer
Q86XT4
-
trimer
Q810I2
-
monomer
-
1 * 115000, SDS-PAGE
additional information
-
Cul1, Cul3 and Cul4 proteins homooligomerize in intact cells, suggesting that the respective cullin-based E3 ligases are dimeric
additional information
-
AIP2 contains four WW domains that specifically recognize PPXY motifs
additional information
-
Neur domain architecture and sequence comparison, NMR structure determination and analysis, NHR domain structure comparison to other proteins, NHR domain subfamilies, overview. Structure determination and modelling of the binding interface of the NHR1 domain of Neur
additional information
P35131
RMA proteins contain a RING motif and a transmembrane domain in their N-terminal and extreme C-terminal regions, respectively
additional information
-
the enzyme is part of the CUL4A-DDB1-ROC1-beta-TRCP E3 ligase complex
additional information
Mus musculus C57BL/6
-
AIP2 contains four WW domains that specifically recognize PPXY motifs
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CHIP U-box domain complexed with UbcH5a, hanging drop vapour diffusion method, over 1.65 M ammonium sulfate, 90 mM Bis-Tris (pH 6.7), at 20°C
-
sitting drop vapor diffusion method, using 0.1 M MES, pH 6.0, 1.0 M Li2SO4, and 16% glycerol
-
1.9 A crystal structure of the ternary complex of SOCS2 with elongin C and elongin B. Crystal structure of the SOCS2-elongin C-elongin B complex defines a prototypical SOCS box ubiquitin ligase
-
crystal structure of the UBA domain of Cbl-b in complex with ubiquitin at 1.9 resolution
Q13191
crystallization of c-Cbl-UbcH7-ZAP70 peptide ternary complex, crystals are grown at 4°C by hanging drop vapour diffusion by mixing the peptide solution consisting of 27 mg/ml c-Cbl, 32 mg/ml UbcH7 and 40 mg/ml ZAP70 in 20 mM 2-(N-morpholino)ethanesulfonic acid, 150 mM NaCl, 5 mM dithiothreitol, pH 6.5, with an equal volume of reservoir solution containing 100 mM sodium citrate, 1.9-2.1 M ammonium sulfate, pH 5.6, crystals diffract to 2.9 A
-
in complex with the HIV virion infectivity factor, hanging drop vapour diffusion method, in 0.1 M Tris-HCl (pH 7.0) and 40% PEG 350 monomethyl ether
-
sitting drop vapor diffusion method, using in 0.1 M HEPES, pH 6.5, and 2 M ammonium sulfate at 20°C
Q9HCE7
crystallization of Siah in complex with the synthetic PHYL peptide 107-130 (Leu-Gln-Gln-Glu-Arg-Thr-Lys-Leu-Arg-Pro-Val-Ala-Met-Val-Arg-Pro-Thr-Val-Arg-Val-Gln-Pro-Gln-Leu-NH2), hanging-drop vapor-diffusion technique
P61092
sitting-drop vapor diffusion method. Crystal structures of the Skp1-Fbs1 complex and the sugar-binding domain (SBD) of the Fbs1-glycoprotein complex
-
crystals of the U-box domain, Prp19(1-58) are prepared in hanging-drop vapor-diffusion experiments. The data strongly support the contention that the Prp19 U-box exists in a dimeric state in the context of intact Prp19
-
sitting drop vapour diffusion method, using 2 M ammonium sulfate and 1% (w/v) PEG MME 2000
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
cyan fluorescent protein has little effect on the ubiquitin ligase (E3) enzyme activity
-
removing the RING stabilizes XIAP in apoptotic thymocytes, demonstrating that XIAP ubiquitin-ligase activity is a major determinant of XIAP protein stability
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amylose resin chromatography; amylose resin chromatography
Q84TG3, Q9SVC6
glutathione agarose column chromatography
-
recombinant enzyme from Escherichia coli as maltose binding protein-fusion protein by amylose affinity chromatography
P35131
glutathione Sepharose column chromatography, gel filtration
-
Ni-NTA column chromatography
-
glutathione Sepharose column chromatography and Superdex 200 gel filtration
-
amylose resin chromatography
-
amylose resin chromatography and TALON affinity chromatography; amylose resin chromatography and TALON affinity chromatography
Q9C035
amylose resin column chromatography
Q5T447
glutathione Sepharose column chromatography
Q00987
glutathione-agarose column chromatography
-
glutathione-Sepharose column chromatography
-
Ni-NTA agarose chromatography
-
Ni-NTA agarose column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
Q9HCE7
protein A-Sepharose chromatography
P46934
protein G Sepharose column chromatography
-
recombinant c-Cbl
-
recombinant c-Clb
-
recombinant CNOT4
-
recombinant GST-fused Roc1/Rbx1, CUL1, Skp1, and Fbw7 proteins from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, proteolytic removal of the GST-tag
-
recombinant GST-fusion protein
-
recombinant GST-tagged isozyme Pirh2A from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration; recombinant GST-tagged isozyme Pirh2B from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration; recombinant GST-tagged isozyme Pirh2C from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration
Q96PM5
recombinant His-tagged enzyme from Hi-5 cells by His affinity chromatography or from Sf9 cells by anion exchange chromatography and gel filtration
-
recombinant Mdm2
-
recombinant ICP0
-
-
P61092
glutathione affinity chromatography; glutathione affinity chromatography; glutathione affinity chromatography
P35226, Q64028, Q9CQJ4
glutathione-Sepharose column chromatography
Q9D5V5
Ni-NTA column chromatography
O08759
recombinant
-
recombinant ARNIP
Q9CR50
recombinant Skp1–Fbs1 complex
-
recombinant EL5
Q8S919
Ni-NTA agarose column chromatography
O88846
GST-TRAP column chromatography
-
Ni-NTA column chromatography
-
using Ni-NTA chromatography
-
3 structurally related enzyme forms
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as glutathione-S-transferase fusion proteins in Escherichia coli strains BL21 AI or BL21-pLysS
-
expressed in Escherichia coli strain BL21(DE3)
Q9LQ92
expressed in Escherichia coli; expressed in Escherichia coli
Q84TG3, Q9SVC6
RMA1-3, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli as maltose binding protein-fusion protein, in vivo targeting experiments using an Arabidopsis thaliana protoplast-transfection system and GFP-tagged RMAs
P35131
transient expression of PUB17 in Cf-9 tobacco cells silenced for ACRE276 restores hypersensitive response
-
expressed in HEK-293 cells
Q18223
expressed in Escherichia coli
-
expressed in Escherichia coli Rosetta2(DE3) cells
-
phylogenetic tree, expression of Neur in Escherichia coli
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
-
expression in Escherichia coli
-
expressed in Escherichia coli JM109 cells
-
cloning of cDNA
-
expressed in 293 cells
-
expressed in a baculovirus-based expression system and in U2OS cells
-
expressed in COS-7 cells
Q94297
expressed in COS-7 cells, HeLa cells, Hep-G2 cells, and HEK-293T cells
Q86XT4
expressed in COS-7, H-1299 and SAOS-2 cells
Q76N89
expressed in Escherichia coli and in HEK-293T cells; expressed in Escherichia coli and in HEK-293T cells
Q9C035
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(Star) cells
-
expressed in Escherichia coli Rosetta cells fused to the maltose binding protein
-
expressed in Escherichia coli strain SCS1
Q9P0P0
expressed in Escherichia coli Top10 cells
Q14669
expressed in HEK-293 cells
-
expressed in HEK-293 cells and in Escherichia coli strain BL21(DE3)
Q68DV7
expressed in HEK-293T cells
-
expressed in HeLa cells
Q8IU81
expressed in JHU 022 and H-1299 cells
-
expressed in Mus musculus
Q05086
expressed in NIH 3T3 and HeLa cells
P46934
expressed in NIH-3T3 cells
Q969V5
expressed in Saccharomyces cerevisiae
Q5T447
expressed in Saccharomyces cerevisiaer MaV203 cells and HEK293 cells
-
expressed in U2OS cells
-
expression in Sf9 insect cells
-
expression in SH-SY5Y cells and HEK293 cells
-
expression of c-Cbl residues 47-447 in Escherichia coli
-
expression of GFP-gp78 fusion protein in U2OS cells, expression of gp78 in HEK 293T cells
-
expression of GST-c-Clb fusion protein encompassing residues 359-447 of cClb in Escherichia coli
-
expression of GST-fused Roc1/Rbx1, CUL1, Skp1, and Fbw7 proteins in Escherichia coli strain BL21 (DE3)
-
expression of GST-KIAA0860 fusion protein in Escherichia coli; expression of GST-PRP19 fusion protein in Escherichia coli
-
expression of GST-Mdm2 fusion protein in Escherichia coli
-
expression of His-tagged Triad1 via the baculovirus expression system in Hi-5 or Sf9 insect cells. Expression of wild-type and mutant Triad1 in U-937 cells, the wild-type enzyme inhibits the clonogenic growth of the cells. UbcH7 and Ubc13 bait and Triad1 prey deletion constructs are transfected to the Saccharomyces cerevisiae strain AH109 for yeast-two-hybrid assays
-
human SOCS2 (amino acids 32–198), elongin C (amino acids 17–112), and elongin B are coexpressed in Escherichia coli BL21(DE3)
-
isozyme Pirh2A, transient expression of GFP-tagged Pirh2A in HelA cells and HEK-293T cells, and of Myc-tagged Pirh2A in HCT-116 cells, expression of GST-tagged isozyme in Escherichia coli strain BL21(DE3), coexpression in H-1299 cells with isozymes Pirh2B and/or Pirh2C; isozyme Pirh2B, DNA and amino acid sequence determination and analysis, transient expression of GFP-tagged Pirh2B in HelA cells and HEK-293T cells, and of Myc-tagged Pirh2B in HCT-116 cells, expression of GST-tagged isozyme in Escherichia coli strain BL21(DE3), coexpression in H-1299 cells with isozymes Pirh2A and/or Pirh2C; isozyme Pirh2C, DNA and amino acid sequence determination and analysis, transient expression of GFP-tagged Pirh2C in HelA cells and HEK-293T cells, and of Myc-tagged Pirh2C in HCT-116 cells, expression of GST-tagged isozyme in Escherichia coli strain BL21(DE3), coexpression in H-1299 cells with isozymes Pirh2B and/or Pirh2A
Q96PM5
mahoganoid, four splicing variants, expression of FLAG-tagged wild-type and mutant MGRN-1 isozymes in HEK-293T cells. Expression of the MGRN1s in HBL melanoma cells
O60291
mouse Ube2g2 (conjugating enzyme E2) and human gp78 (ligase E3), an endoplasmic reticulum (ER)-associated conjugating system essential for the degradation of misfolded ER proteins, are recombinantly expressed in Escherichia coli
-
overexpression of FLAG-tagged Parkin in 293(T) cells and SH-SY5Y cells
-
UBA domain from Cbl-b (residues 924–973) was cloned into a pGEX-4T-1 vector (Amersham-Pharmacia) and expressed in Escherichia coli BL21 (DE3)
Q13191
expressed in Vero, HEp-2, U2-OS, and HeLa cells
-
expression of GST-ICP0-exon2 and exon 3 in fibroblasts
-
expressed in HEK-293T cells
-
expressed in CHO cells
Q5ZRQ0
cloning of cDNA, expression of GST-mARNIP fusion protein in escherichia coli
Q9CR50
coexpression of AIP2 with EGR2 in HEK-293T cells, expression of a GST-tagged cDNA fragment corresponding to four WW domains of AIP2, amino acids 281 to 500, in Escherichia coli strain BL21
-
expressed in 293T, NIH-3T3, and COS-7 cells
Q3U827
expressed in Escherichia coli BL21(DE3) cells
Q9D5V5
expressed in HEK-293 cells
-
expressed in HEK-293T cells
Q8C863
expressed in HEK-293T cells and Escherichia coli BL21 cells; expressed in HEK-293T cells and Escherichia coli BL21 cells
A2A5Z6
expressed in MEF cells
-
expressed in Sf9 cells
Q5RJY2
expressed in U-2 OS cells, HEK-293 cells, and Sf9 insect cells, Rae28 and Scmh1 are unstable in Sf9 cells; expressed in U-2 OS cells, HEK-293 cells, and Sf9 insect cells, Rae28 and Scmh1 are unstable in Sf9 cells; expressed in U-2 OS cells, HEK-293 cells, and Sf9 insect cells, Rae28 and Scmh1 are unstable in Sf9 cells
P35226, Q64028, Q9CQJ4
expressed in U20S cells
Q810I2
expression in HWK-293 T cells
-
expression of GST-CHIP fusion protein in Escherichia coli; expression of GST-UFD2a fusion protein in Escherichia coli; expression of GST-UFD2b fusion protein in Escherichia coli
O94941, Q9WUD1
mahoganoid, expression of FLAG-tagged wild-type and mutant MGRN-1 in HEK-293T cells
-
Siah 1a (92-282) is cloned into PMCSG7 and expressed as a His-tag fusion protein in BL21 DE3 Rosetta cells
P61092
the Skp1-Fbs1 complex is coexpressed from the pET28b plasmid in Escherichia coli BL21
-
transient expression of full-length and HECT domain-deleted forms of E6-AP in Cos-7 cell and in murine neuro 2a cells
-
expression of MBP-EL5 fusion protein in Escherichia coli
Q8S919
expressed in Escherichia coli Rosetta (DE3) cells
O88846
expressed in HEK-293T cells
-
expressed in Saccharomyces cerevisiae
-
expression of staring in Escherichia coli and HeLa cells
Q8CJB9
Myc-tagged Pam F3 is expressed in HEK-293T cells
-
expressed in Escherichia coli BL21-RIL cells
-
expression in Sf9 insect cells
-
overexpression in Schizosaccharomyces pombe
Q9UTG2
expressed in Escherichia coli BL21-Gold (DE3) cells
-
expression in Saccharomyces cerevisiae
-
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Saccharomyces cerevisiae
-
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Triad1 is induced on differentiation towards monocytes and granulocytes
-
MURF1 shows strong postnatal up-regulation in heart
-
ghrelin inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx
-
thermal injury induces the enzyme expression in skeletal muscle
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C13A
-
the mutation within the U-box motif abolishes the ligase activity
V24I
Q84TG3, Q9SVC6
the PUB22 mutant fails to ubiquitinate RPN12a
D229A
-
the mutant is less active in polyubiquitin chain formation by Ubc13-Uev1a than any CHIP mutants other than I235A, the mutation has little effect on CHIP autoubiquitination by UbcH5b
I235A
-
the mutant is inactive with the E2 enzymes UbcH5 and Ubc13-Uev1a
G167E
-
mutation of the Neur NHR1 domain residue G167 critically affects the Neur activity due to sterical problems and conformational changes in the protein
R42L
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
R54L
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
R74L
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
R42L
Escherichia coli AR58
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
-
R54L
Escherichia coli AR58
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
-
R74L
Escherichia coli AR58
-
4 Arg present on ubiquitin at positions 42, 54, 72, and 74 are independently mutated to Leu. All of the mutants except UbR54L exhibit altered kinetics for E1-catalyzed ATP-diphosphate exchange compared to wild-type ubiquitin. UbR72L mutant alters the mechanism of E1 from strictly order addition of substrates to random addition with respect to ATP and ubiquitin
-
G1321A
-
the WWP1 missense mutations is responsible for chicken muscular dystrophy
R441Q
-
the WWP1 missense mutations is specific for chicken muscular dystrophy
A30P
-
the Parkinson's disease mutation does not alter monoubiquitination of alpha-synuclein
A53T
-
the Parkinson's disease mutation does not alter monoubiquitination of alpha-synuclein
C136F
-
the mutation mimics cysteine oxidation/alkylation and results in constitutive binding of UbcM2 to the transcription factor Nrf2 and an increased half-life of the transcription factor in vivo
C145S
-
the mutant forms a beta-mercaptoethanol-resistant oxyester bond with ubiquitin
C15A
-
the mutant does not ubiquitinate itself
C339A
Q969V5
mitochondrial localization-defective MULAN mutant
C372A
-
similar ubiquitin-protein ligase activity as wild-type
C373T
-
no decrease of Mdm2 ubiquitination
C381A
-
no ubiquitin-protein ligase activity
C4341A
-
the mutant shows no ubiquitin ligase activity towards N-Myc
C438L
-
complete loss of Mdm2 and p53 ubiquitination
C449S
-
no decrease of Mdm2 ubiquitination
C461S
-
complete loss of Mdm2 and p53 ubiquitination
C464A
-
complete loss of Mdm2 and p53 ubiquitination
C464S
-
catalytically inactive mutant
C475G
-
complete loss of Mdm2 and p53 ubiquitination
C478S
-
complete loss of Mdm2 and p53 ubiquitination
C699A
Q9HCE7
inactive
E46K
-
the Parkinson's disease mutation does not alter monoubiquitination of alpha-synuclein
H452A
-
complete loss of Mdm2 and p53 ubiquitination
H457S
-
complete loss of Mdm2 and p53 ubiquitination
I26A
P38398
the mutant does not restore DNA damage-induced CtIP focus formation in BRCA1-deficient cells
K161N
-
pathogenic mutant, mutation does not abrogate the ubiquitination of the p38 subunit of the aminoacyl-tRNA synthetase complex
P76C
-
the mutation fails to confer Nrf2 binding by UbcM2
R260A/K261A
Q969V5
mitochondrial localization-defective MULAN mutant
T455A
-
49% of wild-type ubiquitinqtion activity
C833A
-
the dominant negative E6AP mutant is catalytically inactive
C145A
Q9CR50
no ubiquitin ligase activity
C147A
Q5RJY2
the mutant has no E3 ligase activity
C168S
-
mutation in the N-terminal RING domain of MEX exhibits decreased ubiquitination
C258A/C261A
Q5RJY2
the mutant has no E3 ligase activity
C292A
O94941, Q9WUD1
100% of wild-type ubiquitin-protein ligase activity
C379A
P22682
the mutation leads to abolished E3 ligase activity and subsequent hyperphosphorylation and accumulation if STAT5 protein in hematopoietic stem cells
C5A
Q5RJY2
the mutant has no E3 ligase activity
C666A
Q5RJY2
the mutant has no E3 ligase activity
C66S |
-
mutation in the SWIM domain almost abolishes the ubiquitination of MEX
C833A
-
ligase defective mutant
C84A
Q5RJY2
the mutant has no E3 ligase activity
H261A
O94941, Q9WUD1
no ubiquitin-protein ligase activity
P1140A
O94941, Q9WUD1
no ubiquitin-protein ligase activity
P270A
O94941, Q9WUD1
no polyubiquitination activity
C153S
Q8S919
no ubiquitin-protein ligase activity
K122R
Q8S919
sole lysine of EL5 is changed in MBP-EL5 fusion protein, no effect on auto-ubiquitination of MLB-EL5
C4394A/C4409A/C4417A
-
the mutations in the C-terminal RING finger domain completely abolish self-ubiquitination of Pam, the mutations result in significant prolongation of Pam's half-life (about 50% of wild type Pam F3 is degraded within 3-6 h)
C639A
Q9UTG2
Cys639 forms a thioester bond with ubiquitin
C337A
-
the mutant fails to rescue sst2DELTA cells for growth in the presence of pheromone indicating that the presence of a thiol group at this position is essential for IpaH activity, the mutant is not impaired in its ability to bind the E2 enzyme UBE2D2
C337S
-
the mutant fails to rescue sst2DELTA cells for growth in the presence of pheromone indicating that the presence of a thiol group at this position is essential for IpaH activity, the mutant is not impaired in its ability to bind the E2 enzyme UBE2D2
C368A
-
the mutation dramatically impaires enzymatic activity
C626X
-
substitution of the cysteine at position 626 abolishes activity
W40A
-
the mutation within the U-box motif abolishes the ligase activity
additional information
Q18223
fsn-1(gk429) mutants are as slightly sensitive to 2.5 mM N-ethyl-N-nitrosourea and cep-1, fsn-1 double mutants are moderately sensitive to 1.0 mM N-ethyl-N-nitrosourea compared with cep-1 single mutants
K28A/K85A
Q9HCE7
the half-life of RhoA is dramatically longer in the presence of the Smurf1 K28A/K85A mutant than in the presence of wild type Smurf1, but the Smurf1 K28A/K85A mutation has no significant effects on the half-life of Smurf1 itself
additional information
-
897insA, 2544insA and E167X mutations are found in patients with Angelman syndrome
additional information
-
ubiquitination of the p38 subunit of the aminoacyl-tRNA synthetase complex is abrogated by truncated variants of Parkin lacking the essential functional domains
additional information
-
a HRD1 mutant defective in the transmembrane domain is markedly more unstable than wild type HRD1
additional information
O60291
expression of the MGRN1 isozymes in HBL melanoma cells decrease significantly the endogenous MC1R signaling. In HEK cells transfected to express MGRN1 isoforms and MC4R, signaling is strongly inhibited, independent on receptor downregulation or ubiquitylation
additional information
-
generation of Triad1 mutants DELTARING1, DELTADRIL, DELTARING2 and DELTATRIAD using sexual PCR, RING1 abrogates the interaction with UbcH7, whereas deletion of RING2 does not disturb the UbcH7 interaction, but the other RING finger, RING2, is essential for Ubc13 interaction, the deletion of the complete TRIAD domain abolishes the interaction with both UbcH7 and Ubc13
additional information
-
Hdm2 overexpression in cultured cells causes a decrease in Ku70 expression levels
W408A
-
weak binding to Ubc4
additional information
-
mutations within one or more of the phosphorylated regions impair the ability of ICP0 to form foci with colocalizing conjugated ubiquitin and to disrupt the substrate nuclear domain 10, the ICP0 phosphorylation mutant P1 shows a significant defect in viral replication and enhanced protein stability
H333A
Q8WZ73
the mutation abrogates ubiquitin ligase E3 activity
additional information
P22682
c-Cbl mutant hematopoietic stem cells show hyperproliferation in vivo and are hyperresponsive to thrombopoietin in vitro
additional information
Q8VBV4
genetic loss of Fbw7 severely impairs early T cell development, leads to a cell-autonomous defect of stem cell self-renewal, leads to a depletion of bone marrow hematopoietic progenitors, severely affects progenitor maintenance in the thymus, severely affects hematopoietic progenitor maintenance in the bone marrow, and leads to defective HSC cell quiescence and self-renewal
additional information
O08759
knockdown of E6-AP induces cell death
additional information
-
mice deficient in TRIM2 have increased neurofilament light subunit level in axons and neurofilament light subunit filled axonal swellings in cerebellum, retina, spinal cord, and cerebral cortex
additional information
-
the DELTARING mutation causes increased apoptosis and increased organismal survival in the Emu-Myc mouse lymphoma model
additional information
-
AIP2 overexpression significantly enhances EGR2 ubiquitination, but its overexpression fails to promote ubiquitin conjugation onto the EGR2 mutant Y2F, suppression of AIP2 expression by small RNA interference upregulates EGR2, inhibits EGR2 ubiquitination and FasL expression, and enhances the apoptosis of T cells
additional information
-
conditional inactivation of the Huwe1 gene in the mouse brain causes neonatal lethality associated with disorganization of the laminar patterning of the cortex. These defects stem from severe impairment of neurogenesis associated with uncontrolled expansion of the neural stem cell compartment, N-myc and DLL3 are responsible for the defects in the Huwe1 null brain, loss- and gain-of-function experiments in the mouse cortex, overview. Deletion of Huwe1 deregulates proliferation and impairs cell cycle withdrawal and lengthening of cell cycle Ttming of cortical progenitors. Recruitment of active notch signaling by N-myc protein accumulation in Huwe1 knockout brain
additional information
-
overexpression of E6-AP protects against endoplasmic reticulum stress-induced cell death. Deletion of HECT domain of E6-AP not only blocked the degradation of denatured luciferase but also probably increased its refolding
P306A
O94941, Q9WUD1
no ubiquitin-protein ligase activity
additional information
Mus musculus C57BL/6
-
AIP2 overexpression significantly enhances EGR2 ubiquitination, but its overexpression fails to promote ubiquitin conjugation onto the EGR2 mutant Y2F, suppression of AIP2 expression by small RNA interference upregulates EGR2, inhibits EGR2 ubiquitination and FasL expression, and enhances the apoptosis of T cells
-
D339A/D397A/R340A
-
the mutant fails to rescue or only partially rescues growth of sst2DELTA cells, and is still able to bind the E2 enzyme UBE2D2
additional information
-
overexpression of Rsp5p inhibits TBSV replication in Saccharomyces cerevisiae, while downregulation of Rsp5p leads to increased TBSV accumulation
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
Q6ZNA4, Q96PU5
abnormalities in E3 ubiquitin ligases that control components of TGF-beta family signaling may lead rrto the development and progression of various cancers
medicine
-
abnormalities in E3 ubiquitin ligases that control components of TGF-beta family signaling may lead to the development and progression of various cancers
medicine
-
E3 ligases can be considered as bona fida targets to interfere with HIV infection
medicine
-
high nuclear EDD expression is associated with an approximately 2fold increased risk of disease recurrence and death in patients with serous ovarian carcinoma
medicine
Q05086
loss of E6-AP function by mutation is responsible for the development of Angelman syndrome, deregulation of E6-AP expression and activity contributes to autism
medicine
-
monoubiquitination of alpha-synuclein plays an important role for Lewy body formation, decreasing alpha-synuclein monoubiquitination, by preventing SIAH function or by stimulating autophagy, is a therapeutic strategy for Parkinson's disease
medicine
-
submicroscopic duplication of the E3 ubiquitin ligase HUWE1 is associated with mental retardation
medicine
-
the BRCA1 RING domain is a potentially molecular target for platinum-based drugs for cancer therapy
medicine
-
Nedd4-2 is a critical regulator of epithelial Na+ channel subunit activity and blood pressure, variation in the Nedd4-2 gene contributes to the etiology of salt-sensitive hypertension
medicine
-
the XIAP RING may be a promising drug target for developing a novel class of cancer therapeutics since there is a physiological requirement of XIAP ubiquitin-ligase activity for the inhibition of caspases and for tumor suppression in vivo, deletion of the RING from XIAP improves the survival of mice in the Emu-Myc lymphoma model