Information on EC 6.3.2.16 - D-alanine-alanyl-poly(glycerolphosphate) ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Lactobacillus casei

EC NUMBER
COMMENTARY hide
6.3.2.16
-
RECOMMENDED NAME
GeneOntology No.
D-alanine-alanyl-poly(glycerolphosphate) ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + D-alanine + alanyl-poly(glycerolphosphate) = ADP + phosphate + D-alanyl-alanyl-poly(glycerolphosphate)
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
-
-
-
-
carboxylic acid amide formation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-Alanine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
D-alanine:alanyl-poly(glycerolphosphate) ligase (ADP-forming)
Involved in the synthesis of teichoic acids.
CAS REGISTRY NUMBER
COMMENTARY hide
9046-58-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + D-Ala + membrane-acceptor
AMP + phosphate + D-alanyl-membrane acceptor
show the reaction diagram
-
83% of the activity relative to ATP
-
-
-
ATP + D-2-amino-n-butanoate + membrane-acceptor
ADP + phosphate + D-2-aminobutanoyl-membrane acceptor
show the reaction diagram
-
-
-
-
-
ATP + D-Ala + membrane-acceptor
?
show the reaction diagram
-
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
-
-
-
ATP + D-Ala + membrane-acceptor
ADP + phosphate + D-alanyl-membrane acceptor
show the reaction diagram
-
no activity with L-Ala
-
-
CTP + D-Ala + membrane-acceptor
CDP + phosphate + D-alanyl-membrane acceptor
show the reaction diagram
-
107% of the activity relative to ATP
-
-
-
GTP + D-Ala + membrane-acceptor
GDP + phosphate + D-alanyl-membrane acceptor
show the reaction diagram
-
84% of the activity relative to ATP
-
-
-
UTP + D-Ala + membrane-membrane acceptor
UDP + phosphate + D-alanyl-membrane acceptor
show the reaction diagram
-
126% of the activity relative to ATP
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-Ala + membrane-acceptor
?
show the reaction diagram
-
reaction is responsible for the incorporation of D-Ala into membranes of Lactobacillus casei, involved in the synthesis of teichoic acids
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Ca2+
-
stimulates
Co2+
-
stimulates
Mg2+
-
stimulates
Mn2+
-
stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-2-amino-n-butanoate
-
-
DL-2-Amino-n-butanoyl hydroxamate
-
-
DL-Ala amide
-
-
DL-Alanine hydroxamate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.85
D-2-amino-n-butanoate
-
-
0.018
D-Ala
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
-
gel filtration