Information on EC 6.3.2.10 - UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase

Word Map on EC 6.3.2.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.2.10
-
RECOMMENDED NAME
GeneOntology No.
UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
carboxylic acid amide formation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine biosynthesis
-
-
Metabolic pathways
-
-
Peptidoglycan biosynthesis
-
-
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
-
-
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
-
-
peptidoglycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine:D-alanyl-D-alanine ligase (ADP-forming)
Involved with EC 6.3.2.4 (D-alanine---D-alanine ligase), EC 6.3.2.7 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase) or EC 6.3.2.13 (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---2,6-diaminopimelate ligase), EC 6.3.2.8 (UDP-N-acetylmuramate---L-alanine ligase) and EC 6.3.2.9 (UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase) in the synthesis of a cell-wall peptide (click here) for diagram. This enzyme also catalyses the reaction when the C-terminal residue of the tripeptide is meso-2,4-diaminoheptanedioate (acylated at its L-centre), linking the D-Ala-D-Ala to the carboxy group of the L-centre. This activity was previously attributed to EC 6.3.2.15, which has since been deleted.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-60-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain K37
SwissProt
Manually annotated by BRENDA team
9602
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
?
show the reaction diagram
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
show the reaction diagram
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Lac
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Lac
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + fluoro-D-Ala-fluoro-D-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-fluoro-D-Ala-fluoro-D-Ala
show the reaction diagram
-
-
-
-
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-diaminopimelic acid + D-alanyl-D-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-diaminopimeloyl-D-alanyl-D-alanine
show the reaction diagram
-
-
-
-
?
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
show the reaction diagram
additional information
?
-
the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
?
show the reaction diagram
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
show the reaction diagram
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine
ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
show the reaction diagram
additional information
?
-
P11880
the specific activity of the mutant enzyme is highly reduced compared to the wild-type murF
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,4R)-4-(3,7-dimethoxynaphthalen-2-yl)-1-(dimethylamino)-3,4-diphenylbutan-2-ol
-
modest activity against lipopolysaccharide-defective Escherichia coli and wild-type Escherichia coli rendered permeable with polymyxin B nonapeptide. Treatment of lipopolysaccharide-defective Escherichia coli cells with >2fold minimal inhibitory concentrations of DQ1 results in a 75-fold-greater accumulation of the MurF substrate compared to the control, a 70% decline in the amount of the MurF product, and eventual cell lysis, consistent with the inhibition of MurF within bacteria
(2S,4R)-4-(3,7-dimethoxynaphthalen-2-yl)-1-(dimethylamino)-3,4-diphenylbutan-2-ol
-
additionally active against Gram-positive bacteria, including methicillin-susceptible and -resistant Staphylococcus aureus isolates and vancomycin-susceptible and -resistant Enterococcus faecalis and Enterococcus faecium isolates
1,4-diaminoanthra-9,10-quinone
-
pIC50 predicted by comparative residue interaction analysis: 5.0, pIC50 predicted by comparative molecular field analysis:5.5
1-(azepan-1-ylmethyl)-2-naphthol
-
pIC50 predicted by comparative residue interaction analysis: 5.7, pIC50 predicted by comparative molecular field analysis: 5.9
1-(cyclohexylmethyl)piperazine
-
pIC50 predicted by comparative residue interaction analysis: 5.5, pIC50 predicted by comparative molecular field analysis: 6.2
1-[[(4-trifluoromethylphenyl)sulfonyl]amino]-3-(morpholin-4-yl)propan-2-yl dihydrogen phosphate
-
-
2,4-dichloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2,4-dichloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
-
-
2,4-dichloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2,4-dichloro-N-(3-cyano-6-phenyl-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-[(2-methoxyethyl)(methyl)sulfamoyl]benzamide
-
-
2,4-dichloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(diethylsulfamoyl)benzamide
-
-
2,4-dichloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-(4-methylphenyl)pyrrolo[2,1-a]isoquinoline
-
pIC50 predicted by comparative residue interaction analysis: 5.1, pIC50 predicted by comparative molecular field analysis: 5.6
2-(propylamino)ethyl 4-amino-3-propoxybenzoate
-
-
2-([[2-chloro-5-(diethylsulfamoyl)phenyl]carbonyl]amino)-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophene-3-carboxamide
-
-
2-bromo-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-2-thienyl)-5-[(diethylamino)sulfonyl]benzamide
-
-
2-chloro-N-(3-cyano-3,4,5,6-tetrahydro-2H-cyclopenta[b]thiophen-2-yl)-5-[(4-hydroxybutyl)sulfamoyl]benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-4-fluoro-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(methylamino)sulfonyl]benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[[(3-hydroxypropyl)amino]sulfonyl]benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophen-2-yl)-4-(methylamino)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-(diethylsulfamoyl)benzamide
-
-
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-(piperidin-1-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-yl)-5-(diethylsulfamoyl)benzamide
-
-
2-chloro-N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
-
-
2-chloro-N-[3-cyano-5-(2-pyridin-2-ylethyl)thiophen-2-yl]-5-(morpholin-4-ylsulfonyl)benzamide
-
-
2-chloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(diethylsulfamoyl)benzamide
-
-
2-thioxo-3-[4-(trifluoromethoxy)phenyl]-2,3-dihydroquinazolin-4(1H)-one
-
-
3-anilinocyclohex-2-en-1-one
-
pIC50 predicted by comparative residue interaction analysis: 5.2, pIC50 predicted by comparative molecular field analysis: 5.5
3-imino-3,4-dihydroisoquinolin-1(2H)-one
-
pIC50 predicted by comparative residue interaction analysis: 5.2, pIC50 predicted by comparative molecular field analysis: 5.6
4,6-dimethyl-1-[[(1E)-phenylmethylene]amino]pyridin-2(1H)-one
-
pIC50 predicted by comparative residue interaction analysis: 5.1, pIC50 predicted by comparative molecular field analysis: 4.6
4-(4-methylpiperazin-1-yl)aniline
-
pIC50 predicted by comparative residue interaction analysis: 5.7, pIC50 predicted by comparative molecular field analysis: 6.1
4-[(pentachlorobenzyl)sulfanyl]-6-sulfanyl-1,3,5-triazin-2-ol
-
i.e. NSC 209931, inhibitor identified by structure-based virtual screening
5-(aminosulfonyl)-2-chloro-N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thien-2-yl)benzamide
-
-
5-(anilinosulfonyl)-2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)benzamide
-
-
6-piperazin-1-yl-1H-benzo[de]isoquinoline-1,3(2H)-dione
-
pIC50 predicted by comparative residue interaction analysis: 5.4, pIC50 predicted by comparative molecular field analysis: 5.8
acetate
-
inhibition of wild-type enzyme
Butylamine
-
inhibition of wild-type and mutant K202A; inhibition of wild-type enzyme
Butyrate
-
slight inhibition of wild-type enzyme
ethyl 2-([[2-chloro-5-(diethylsulfamoyl)phenyl]carbonyl]amino)-3-cyano-4,5,6,7-tetrahydro-1-benzothiophene-6-carboxylate
-
-
ethylamine
-
inhibition of wild-type enzyme
formate
-
inhibition of wild-type enzyme
methylamine
-
inhibition of wild-type enzyme
N-(3-aminopropyl)-4-(trifluoromethyl)-2-([4-[3-(trifluoromethyl)phenyl]-1,3-thiazol-2-yl]methyl)pyrimidine-5-carboxamide
-
-
N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophen-2-yl)-2-[[3-(dimethylamino)propyl]amino]-5-(morpholin-4-ylsulfonyl)benzamide
-
-
N-(4-carbamimidoylphenyl)-4-(trifluoromethyl)-2-([4-[3-(trifluoromethyl)phenyl]-1,3-thiazol-2-yl]methyl)pyrimidine-5-carboxamide
-
-
N-(5-bromo-3-cyano-2-thienyl)-2-chloro-5-[(diethylamino)sulfonyl]benzamide
-
-
N-[3-(cyclopentylamino)propyl]-2-[[4-(4-methylphenyl)-1,3-thiazol-2-yl]amino]-4-(trifluoromethyl)pyrimidine-5-carboxamide
-
-
propionate
-
inhibition of wild-type enzyme
Propylamine
-
inhibition of wild-type enzyme
additional information
-
design of inhibitors of the MurF enzyme of Streptococcus pneumoniae using docking, 3D-QSAR, and de dovo design
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
activation of mutant K202A
Butyrate
-
activation of mutant K202A
ethylamine
-
slight activation of mutant K202A
formate
-
slight activation of mutant K202A
propionate
-
strong activation of mutant K202A
Propylamine
-
slight activation of mutant K202A
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Escherichia coli
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
(2R,4R)-4-(3,7-dimethoxynaphthalen-2-yl)-1-(dimethylamino)-3,4-diphenylbutan-2-ol
Escherichia coli
-
-
0.15
1-[[(4-trifluoromethylphenyl)sulfonyl]amino]-3-(morpholin-4-yl)propan-2-yl dihydrogen phosphate
Escherichia coli
-
pH 8.0
0.000000302
2,4-dichloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.0000052
2,4-dichloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.001
2,4-dichloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.000000071
2,4-dichloro-N-(3-cyano-6-phenyl-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-[(2-methoxyethyl)(methyl)sulfamoyl]benzamide
Streptococcus pneumoniae
-
-
0.000000068
2,4-dichloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(diethylsulfamoyl)benzamide
Streptococcus pneumoniae
-
-
0.000000022
2,4-dichloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.03
2-(propylamino)ethyl 4-amino-3-propoxybenzoate
Streptococcus pneumoniae
-
-
0.000066
2-([[2-chloro-5-(diethylsulfamoyl)phenyl]carbonyl]amino)-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophene-3-carboxamide
Streptococcus pneumoniae
-
-
0.00000417
2-bromo-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.151
2-chloro-N-(3-cyano-2-thienyl)-5-[(diethylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.022
2-chloro-N-(3-cyano-3,4,5,6-tetrahydro-2H-cyclopenta[b]thiophen-2-yl)-5-[(4-hydroxybutyl)sulfamoyl]benzamide
Streptococcus pneumoniae
-
-
0.0000065
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-4-fluoro-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.0000089
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.0000079
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.0000151
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[(methylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.0000079
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)-5-[[(3-hydroxypropyl)amino]sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.0000617
2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophen-2-yl)-4-(methylamino)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.000001 - 0.0000017
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothien-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
0.00000141
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-(diethylsulfamoyl)benzamide
Streptococcus pneumoniae
-
-
0.0025
2-chloro-N-(3-cyano-4,5,6,7-tetrahydro-1-benzothiophen-2-yl)-5-(piperidin-1-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.000003388
2-chloro-N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-yl)-5-(diethylsulfamoyl)benzamide
Streptococcus pneumoniae
-
-
0.000004169
2-chloro-N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-yl)-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.008
2-chloro-N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thien-2-yl)-5-[(diethylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.006
2-chloro-N-[3-cyano-5-(2-pyridin-2-ylethyl)thiophen-2-yl]-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.000000054
2-chloro-N-[3-cyano-6-(4-hydroxyphenyl)-4,5,6,7-tetrahydro-1-benzothiophen-2-yl]-5-(diethylsulfamoyl)benzamide
Streptococcus pneumoniae
-
-
0.019
2-thioxo-3-[4-(trifluoromethoxy)phenyl]-2,3-dihydroquinazolin-4(1H)-one
Streptococcus pneumoniae
-
-
0.063
4-[(pentachlorobenzyl)sulfanyl]-6-sulfanyl-1,3,5-triazin-2-ol
Escherichia coli
-
pH 8.0, 37°C
0.078
5-(aminosulfonyl)-2-chloro-N-(3-cyano-5,6-dihydro-4H-cyclopenta[b]thien-2-yl)benzamide
Streptococcus pneumoniae
-
-
0.0000257
5-(anilinosulfonyl)-2-chloro-N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thien-2-yl)benzamide
Streptococcus pneumoniae
-
-
0.00000603
ethyl 2-([[2-chloro-5-(diethylsulfamoyl)phenyl]carbonyl]amino)-3-cyano-4,5,6,7-tetrahydro-1-benzothiophene-6-carboxylate
Streptococcus pneumoniae
-
-
0.0075
N-(3-aminopropyl)-4-(trifluoromethyl)-2-([4-[3-(trifluoromethyl)phenyl]-1,3-thiazol-2-yl]methyl)pyrimidine-5-carboxamide
Escherichia coli
-
-
0.000074
N-(3-cyano-4,5,6,6a-tetrahydro-3aH-cyclopenta[b]thiophen-2-yl)-2-[[3-(dimethylamino)propyl]amino]-5-(morpholin-4-ylsulfonyl)benzamide
Streptococcus pneumoniae
-
-
0.0025
N-(4-carbamimidoylphenyl)-4-(trifluoromethyl)-2-([4-[3-(trifluoromethyl)phenyl]-1,3-thiazol-2-yl]methyl)pyrimidine-5-carboxamide
Escherichia coli
-
-
0.0126
N-(5-bromo-3-cyano-2-thienyl)-2-chloro-5-[(diethylamino)sulfonyl]benzamide
Streptococcus pneumoniae
-
-
0.0025
N-[3-(cyclopentylamino)propyl]-2-[[4-(4-methylphenyl)-1,3-thiazol-2-yl]amino]-4-(trifluoromethyl)pyrimidine-5-carboxamide
Escherichia coli
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.006
-
purified recombinant mutant K202A, in presence of butylamine
0.016
-
purified recombinant mutant K202A
0.032
-
purified recombinant mutant K202A, in presence of propylamine
0.04
purified recombinant enzyme, 42°C; purified recombinant murF2 mutant, 42°C
0.046
-
purified recombinant mutant K202A, in presence of ethylamine
0.052
-
purified recombinant mutant K202A, in presence of formate
0.062
purified recombinant enzyme, 30°C; purified recombinant murF2 mutant, 30°C
0.437
-
purified recombinant mutant K202A, in presence of butyrate
0.511
-
purified recombinant mutant K202A, in presence of acetate
0.55
-
purified recombinant wild-type enzyme, in presence of methylamine
0.71
-
purified recombinant wild-type enzyme, in presence of propylamine
0.72
-
purified recombinant wild-type enzyme, in presence of butylamine
0.76
-
purified recombinant wild-type enzyme, in presence of propionate
0.769
-
purified recombinant mutant K202A, in presence of propionate
0.8
-
purified recombinant wild-type enzyme, in presence of ethylamine
0.88
-
purified recombinant wild-type enzyme, in presence of acetate
0.93
-
purified recombinant wild-type enzyme, in presence of formate
1.24
-
purified recombinant wild-type enzyme, in presence of butyrate
1.27
-
purified recombinant wild-type enzyme
11.2
purified recombinant wild-type enzyme, 30°C
13.9
purified recombinant wild-type enzyme, 42°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Acinetobacter baumannii (strain AB307-0294)
Acinetobacter baumannii (strain AB307-0294)
Escherichia coli (strain K12)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 4792, nucleotide sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour diffusion method, purified recombinant enzyme 12.2 mg/ml, in 10 mM Tris-HCl, pH 7.4, 10 mM DTT, plus equal volume of reservoir solution: 0.1 M Bis-Tris propane buffer, pH 9.4, 18% PEG 8000, 0.12 M MgSO4, 10% glycerol, about 20 days, X-ray diffraction structure determination at 2.8 A resolution, and analysis
crystals of MurF with bound inhibitor
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant from strain BL21(DE3)
recombinant wild-type and mutant enzyme from strain JM83
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli or Streptomyces coelicolor
-
expression in strain BL21(DE3)
expression of naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion protein; expression of point mutants, expression of wild-type and naturally occuring mutant allele murF2 with A288T mutation as glutathione S-transferase fusion proteins
overexpression of wild-type and MurF mutant K202A as His-tagged enzymes in strain JM83
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A288T
naturally occuring murF2 allele, expressed as a fusion protein with glutathione S-transferase, is 181fold less catalytically active than the wild-type enzyme at 30°c and even more reduced at 42°C
E158A
site-directed mutagenesis, 4520fold reduced activity compared to the wild-type
E158D
site-directed mutagenesis, 246fold reduced activity compared to the wild-type
E158G
site-directed mutagenesis, over 451fold reduced activity compared to the wild-type
H188A
site-directed mutagenesis, 203fold reduced activity compared to the wild-type
H188D
site-directed mutagenesis, 2990fold reduced activity compared to the wild-type
H188G
site-directed mutagenesis, 214fold reduced activity compared to the wild-type
H188N
site-directed mutagenesis, 1860fold reduced activity compared to the wild-type
K202A
-
site-directed mutagenesis, exchange of highly conserved lysine residue leads to highly reduced activity, activity can be rescued best by addition of propionate or other short-chain carboxylic acids, but only in a small extent by amines
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
MurF is a potential target for antibacterial design because it is unique to bacteria and performs an essential non-redundant function in the bacterial cell
pharmacology
Show AA Sequence (12777 entries)
Longer loading times are possible. Please use the Sequence Search for a specific query.