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Information on EC 6.3.2.1 - pantoate-beta-alanine ligase (AMP-forming) and Organism(s) Lotus japonicus and UniProt Accession O24035

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Lotus japonicus
UNIPROT: O24035 not found.
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The taxonomic range for the selected organisms is: Lotus japonicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
synthetase, pantothenate, pantothenate synthetase, mtbps, pantoate-beta-alanine ligase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Pantothenate synthetase
-
D-Pantoate:beta-alanine ligase (AMP-forming)
-
-
-
-
Pantoate activating enzyme
-
-
-
-
pantoate-beta-alanine ligase
-
-
-
-
Pantoic-activating enzyme
-
-
-
-
Pantothenate synthetase
-
-
-
-
Synthetase, pantothenate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide formation
-
-
-
-
carboxamide formation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-pantoate:beta-alanine ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-49-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-pantoate + beta-alanine
AMP + diphosphate + (R)-pantothenate
show the reaction diagram
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
(R)-pantoate
25°C, pH 8.0
0.44 - 0.98
beta-Alanine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.62 - 6.08
(R)-pantoate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
in Tris-HCl
8
in potassium phosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
50% of maximal activity at pH 7.0, 15% of maximal activity at pH 6.0 and pH 9.0 respectively
7.1 - 9
in Tris-HCl, no activity at pH 7.0, 75% of maximal activity at pH 9.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PANC_LOTJA
308
0
34240
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
2 * 34000, SDS-PAGE
72800
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 34000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, no loss of activity
4°C, 4 weeks, 25% loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant pantothenate snthetase
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Genschel, U.; Powell, C.A.; Abell, C.; Smith, A.G.
The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice)
Biochem. J.
341
669-678
1999
Lotus japonicus (O24035), Oryza sativa (O24210)
-
Manually annotated by BRENDA team