Information on EC 6.3.1.7 - 4-methyleneglutamate-ammonia ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Arachis hypogaea

EC NUMBER
COMMENTARY hide
6.3.1.7
-
RECOMMENDED NAME
GeneOntology No.
4-methyleneglutamate-ammonia ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 4-methylene-L-glutamate + NH3 = AMP + diphosphate + 4-methylene-L-glutamine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid amide hydrolysis
-
-
carboxylic
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
C5-Branched dibasic acid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-methylene-L-glutamate:ammonia ligase (AMP-forming)
Glutamine can act instead of NH3, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
85537-85-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 4-methylene-L-glutamate + glutamine
AMP + diphosphate + glutamate + 4-methylene-L-glutamine
show the reaction diagram
-
about 20% as effective as NH4+
-
-
-
ATP + 4-methylene-L-glutamate + hydroxylamine
AMP + diphosphate + 4-methylene-L-glutamylhydroxamate
show the reaction diagram
-
70% as effective as NH4+
-
-
-
ATP + 4-methylene-L-glutamate + NH3
?
show the reaction diagram
-
enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts
-
-
-
ATP + 4-methylene-L-glutamate + NH4+
AMP + diphosphate + 4-methylene-L-glutamine
show the reaction diagram
additional information
?
-
-
no amide/hydroxylamine exchange
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 4-methylene-L-glutamate + NH3
?
show the reaction diagram
-
enzyme is responsible for the rapid synthesis of 4-methyleneglutamine which occurs in germinating peanuts
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Asp
-
with NH4+ is nitrogen donor
diphosphate
-
in presence of F-
Glu
-
with NH4+ is nitrogen donor
methionine sulfoximine
-
partial
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.7 - 8
4-methylene-L-glutamate
3.7
NH4+
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.1
-
more than 70% of maximal activity at pH 6.6 and 8.1
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
226000
-
sedimentation velocity centrifugation
273000
-
gel filtration
280000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-