Information on EC 6.3.1.4 - aspartate-ammonia ligase (ADP-forming)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.3.1.4
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RECOMMENDED NAME
GeneOntology No.
aspartate-ammonia ligase (ADP-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-aspartate + NH3 = ADP + phosphate + L-asparagine
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid amide hydrolysis
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carboxylic
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SYSTEMATIC NAME
IUBMB Comments
L-aspartate:ammonia ligase (ADP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37318-61-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-Asp + hydroxylamine
ADP + phosphate + beta-aspartylhydroxamate
show the reaction diagram
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maximal activity at 400 mM NH2OH
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ATP + L-Asp + NH4+
ADP + phosphate + L-Asn
show the reaction diagram
ATP + L-aspartate + NH3
ADP + phosphate + L-asparagine
show the reaction diagram
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-
-
-
?
additional information
?
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importance of ASNS protein in the cellular mechanisms that confer drug resistance upon the leukemic cells
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-aspartate + NH3
ADP + phosphate + L-asparagine
show the reaction diagram
-
-
-
-
?
additional information
?
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-
importance of ASNS protein in the cellular mechanisms that confer drug resistance upon the leukemic cells
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-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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can replace Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
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above 400 mM
MoO42-
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Na2HAsO4
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p-chloromercuribenzoate
additional information
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CHOP suppresses the induction of the endogenous ASNS gene and inhibits transcription from a reporter gene driven by the ASNS promoter following activation by ATF4 or amino acid deprivation. The inhibitory effect of CHOP on the ASNS gene is not observed when truncated proteins, encoding either the N- or C-terminal portion of the protein, are expressed, overview
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
ATP
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26
L-Asp
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40
NH4+
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0583
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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Mn2+-stimulated reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 8
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5.2: about 70% of maximal Mn2+-stimulated activity, 8.0: about 35% of maximal Mn2+-stimulated activity
6 - 8.4
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6.0: about 70% of maximal Mg2+-stimulated activity, 8.4: about 60% of maximal Mg2+-stimulated activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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an NCI cell line
Manually annotated by BRENDA team
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leukemia-derived cancer cell line
Manually annotated by BRENDA team
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leukemia-derived cancer cell line
Manually annotated by BRENDA team
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mass spectrometric quantification of asparagine synthetase in circulating leukemia cells from acute lymphoblastic leukemia patients, method development and optimization, overview
Manually annotated by BRENDA team
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leukemia-derived cancer cell line
Manually annotated by BRENDA team
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leukemia-derived cancer cell line
Manually annotated by BRENDA team
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enzyme expression analysis in 19 human ovarian cell lines, overview
Manually annotated by BRENDA team
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ovarian subset of the NCI-60 cell line panel
Manually annotated by BRENDA team
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an NCI cell line
Manually annotated by BRENDA team
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an NCI cell line
Manually annotated by BRENDA team
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an NCI cell line
Manually annotated by BRENDA team
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leukemia-derived cancer cell line
Manually annotated by BRENDA team
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leukemia-derived cnmcer cell line
Manually annotated by BRENDA team
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an NCI cell line
Manually annotated by BRENDA team
additional information
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DNA fingerprinting in ovarian cancer cell lines, overview
Manually annotated by BRENDA team
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression analysis in HEK-293 cells in response to ATF4 or CHOP
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quantitative enzyme expression analysis in Hep-G2 cells in absence or presence of UPR blockers and activators, transcription factor recruitment to the ASNS promoter during the UPR, transcriptional activation of UPR target genes is mediated by three signaling cascades PERK/eIF2alpha/ATF4, ATF6, and IRE1/XBP1, UPR activation does not trigger increased recruitment of Mediator subunits to the ASNS promoter, the IRE1/XBP1 and ATF6 branches of the UPR do not participate in the induction of ASNS transcription, overview
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the first intron of the gene ASNS contains two 14-bp tandem repeat sequences, i.e. 2R. The 14-bp sequence functions as a transcriptional enhancer element as shown by reporter analysis and forms a protein-DNA complex in vitro
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the first intron of the gene ASNS contains two 14-bp tandem repeat sequences, i.e. 2R. Approximately 75% of acute lymphoblastic leukemia samples have the 2R sequence in both alleles, 20% and 3% acute lymphoblastic leukemia samples have three and four 14-bp tandem repeats in one allele, respectively. The other allele has 2R. The tandem repeat sequence is not specific to the leukemia cells but represents a novel germline polymorphism. The 14-bp sequence functions as a transcriptional enhancer element as shown by reporter analysis and forms a protein-DNA complex in vitro