Information on EC 6.3.1.15 - 8-demethylnovobiocic acid synthase

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The expected taxonomic range for this enzyme is: Streptomyces niveus

EC NUMBER
COMMENTARY hide
6.3.1.15
-
RECOMMENDED NAME
GeneOntology No.
8-demethylnovobiocic acid synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin = AMP + diphosphate + 8-demethylnovobiocic acid
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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novobiocin biosynthesis
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Novobiocin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
3-dimethylallyl-4-hydroxybenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming)
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxy-8-methylcoumarin
AMP + diphosphate + novobiocic acid
show the reaction diagram
ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin
AMP + diphosphate + 8-demethylnovobiocic acid
show the reaction diagram
ATP + 3-geranyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxy-8-methylcoumarin
AMP + diphosphate + N-(2,7-dihydroxy-8-methyl-4-oxo-4H-chromen-3-yl)-4-hydroxy-3-[(2E)-6-methylhepta-2,5-dien-1-yl]benzamide
show the reaction diagram
ATP + 4-hydroxy-3-methylbenzoate + 3-amino-4,7-dihydroxy-8-methylcoumarin
AMP + diphosphate + 3-[(2E)-but-2-en-1-yl]-N-(2,7-dihydroxy-8-methyl-4-oxo-4H-chromen-3-yl)-4-hydroxybenzylamide
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 3-dimethylallyl-4-hydroxybenzoate + 3-amino-4,7-dihydroxycoumarin
AMP + diphosphate + 8-demethylnovobiocic acid
show the reaction diagram
-
the enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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the reaction is dependent on the presence of Mg2+ or Mn2+
Mn2+
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the reaction is dependent on the presence of Mg2+ or Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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0.5 mM, reduces the enzyme activity to 1.4% of the maximal activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.131
3-amino-4,7-dihydroxy-8-methylcoumarin
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pH 8.0, 30C
0.0064 - 0.019
3-dimethylallyl-4-hydroxybenzoate
0.36
4-hydroxy-3-methylbenzoate
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pH 7.0, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47
3-dimethylallyl-4-hydroxybenzoate
Streptomyces niveus
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pH 7.0, temperature not specified in the publication
0.36
4-hydroxy-3-methylbenzoate
Streptomyces niveus
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pH 7.0, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
73.4
3-dimethylallyl-4-hydroxybenzoate
Streptomyces niveus
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pH 7.0, temperature not specified in the publication
15357
1
4-hydroxy-3-methylbenzoate
Streptomyces niveus
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pH 7.0, temperature not specified in the publication
42491
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.036
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pH 8.0, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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phosphate buffer
8
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Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56885
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1 * 56885, calculated from sequence
57000 - 58000
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gel filtration
60000
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1 * 60000, His-tagged protein, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, purified enzyme loses 22% of its activity after 2 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpressed as a His6 fusion protein in Escherichia coli
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