Information on EC 6.2.1.B4 - glycine:[acyl-carrier protein] ligase (AMP forming)

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The expected taxonomic range for this enzyme is: Bradyrhizobium japonicum

EC NUMBER
COMMENTARY hide
6.2.1.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
glycine:[acyl-carrier protein] ligase (AMP forming)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + glycine + an [acyl-carrier protein] = AMP + diphosphate + a glycyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycine:[acyl-carrier protein] ligase (AMP forming)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
homologous to the catalytic domain of highly diverged, atypical seryl-tRNA synthetases found only in methanogenic archaea and are deprived of the tRNA-binding domain
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + glycine + [acyl-carrier protein Blr6284]
AMP + diphosphate + glycyl-[acyl-carrier protein Blr6284] + AMP + diphosphate
show the reaction diagram
ATP + glycine + [acyl-carrier protein Bsr0959]
AMP + diphosphate + glycyl-[acyl-carrier protein] + AMP + diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + glycine + [acyl-carrier protein Blr6284]
AMP + diphosphate + glycyl-[acyl-carrier protein Blr6284] + AMP + diphosphate
show the reaction diagram
Q89GR3, Q89VT8
-
-
-
?
ATP + glycine + [acyl-carrier protein Bsr0959]
AMP + diphosphate + glycyl-[acyl-carrier protein] + AMP + diphosphate
show the reaction diagram
Q89GR3, Q89VT8
[acyl-carrier protein] Bsr0959
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
Zn2+ found in the active site is tetracoordinated by three conserved amino acids (Cys131, Glu176, and Cys279) and one water molecule
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0039
[acyl-carrier protein Blr6284]
pH 7.5, 30C
-
0.0019
[acyl-carrier protein Bsr0959]
pH 7.5, 30C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.093
[acyl-carrier protein Blr6284]
Bradyrhizobium japonicum
Q89GR3, Q89VT8
pH 7.5, 30C
-
0.11
[acyl-carrier protein Bsr0959]
Bradyrhizobium japonicum
Q89GR3, Q89VT8
pH 7.5, 30C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.91
[acyl-carrier protein Blr6284]
Bradyrhizobium japonicum
Q89GR3, Q89VT8
pH 7.5, 30C
40021
92.4
[acyl-carrier protein Bsr0959]
Bradyrhizobium japonicum
Q89GR3, Q89VT8
pH 7.5, 30C
40020
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
assay at; assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35942
2 * 35942, calculated from sequence
36803
x * 36803, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 36803, calculated from sequence
dimer
2 * 35942, calculated from sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion experiment, crystal structures of the enzyme in complex with AMP, ATP, and glycyladenylate are determined and refined at resolutions of 2.15, 2.50, and 2.20 A, respectively
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE