Information on EC 6.2.1.B3 - OPC-8:CoA ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
6.2.1.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
OPC-8:CoA ligase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP = 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the 4-coumarate-CoA ligase-like enzyme is a member of the acyl-activating enzyme (AAE) gene family
malfunction
in response to wounding, opcl1 null mutants exhibit reduced levels of jasmonic acid and hyperaccumulation of OPC-8:0
metabolism
the enzyme catalyzes the formation of 3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA, which is subsequently converted to jasmonic acid via three beta-oxidation steps
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12-oxo-phytodienoate + CoA + ATP
12-oxo-phytodienoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
12-oxo-phytodienoic acid + CoA + ATP
12-oxo-phytodienoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
show the reaction diagram
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
show the reaction diagram
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
show the reaction diagram
decanoic acid + CoA + ATP
decanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
dinor-12-oxo-phytodienoic acid + CoA + ATP
dinor-12-oxo-phytodienoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
dodecanoic acid + CoA + ATP
dodecanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
hexadecanoic acid + CoA + ATP
hexadecanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
myristate + CoA + ATP
myristoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
octadecanoic acid + CoA + ATP
octadecanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
oleate + CoA + ATP
oleoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
oleic acid + CoA + ATP
oleoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
tetradecanoic acid + CoA + ATP
tetradecanoyl-CoA + AMP + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12-oxo-phytodienoate + CoA + ATP
12-oxo-phytodienoyl-CoA + AMP + diphosphate
show the reaction diagram
Q84P21
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid + CoA + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoyl-CoA + AMP + diphosphate
show the reaction diagram
Q84P21
-
-
-
?
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate + ATP
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoyl-CoA + AMP + diphosphate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
12-oxo-phytodienoate
pH 7.5, temperature not specified in the publication, recombinant enzyme
0.024 - 0.027
12-oxo-phytodienoic acid
0.094
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid
pH not specified in the publication, temperature not specified in the publication
0.045
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoate
pH 7.5, temperature not specified in the publication, recombinant enzyme
0.019 - 0.045
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid
0.076
dinor-12-oxo-phytodienoic acid
pH not specified in the publication, temperature not specified in the publication
-
0.036
tetradecanoic acid
pH not specified in the publication, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.56
12-oxo-phytodienoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
1.49
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
1.27
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
1.78
dinor-12-oxo-phytodienoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
-
0.96
tetradecanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
56.2
12-oxo-phytodienoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
9303
15.2
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-hexanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
28961
65.4
3-oxo-2-(2'-[Z]-pentenyl)cyclopentane-1-octanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
28960
23.4
dinor-12-oxo-phytodienoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
18872
26.4
tetradecanoic acid
Arabidopsis thaliana
Q84P21
pH not specified in the publication, temperature not specified in the publication
4284
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
wounded leaf and jasmonic acid-treated leaf
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59375
x * 59375, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 59375, calculated
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminally His6-tagged OPCL1 from Escherichia coli strain M15 by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; gene opcl1, DNA and amino acid sequence determination and analysis, expression of YFP-tagged OPCL1 in Arabidopsis thaliana via Agrobacterium tumefaciens strain C58C1 transfection, and coexpression with GFP-tagged 12-oxophytodienoate reductase 3, EC 1.3.1.42, the proteins are targeted to peroxisomes, expression of N-terminally His6-tagged OPCL1 in Escherichia coli strain M15
gene opcl1, expression of YFP-tagged OPCL1 in Arabidopsis thaliana, coexpression with GFP-tagged 12-oxophytodienoate reductase 3, EC 1.3.1.42
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is activated in response to wounding or infection
induction by mechanical wounding
the expression of enzyme gene OPCL1 is tightly coordinated with that of the peroxisomal enzyme OPDA reductase via the jasmonate signaling pathway