Information on EC 6.2.1.B10 - 2-(imidazol-4-yl)acetate-CoA ligase

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The expected taxonomic range for this enzyme is: Thermococcus kodakarensis

EC NUMBER
COMMENTARY hide
6.2.1.B10
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
2-(imidazol-4-yl)acetate-CoA ligase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 2-(imidazol-4-yl)acetate + CoA = ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2-(imidazol-4-yl)acetate:CoA ligase (ADP-forming)
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Q5JEW2: alpha-subunit, Q5JIA9: beta-subunit
Q5JEW2 and Q5JIA9
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-(imidazol-4-yl)acetate + CoA
ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 2-(imidazol-4-yl)acetate + CoA
ADP + phosphate + 2-(imidazol-4-yl)acetyl-CoA
show the reaction diagram
Q5JEW2 and Q5JIA9
the enzyme is involved in catabolism of histidine
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
ATP
Q5JEW2 and Q5JIA9
pH 6.5, 55C
0.0625
CoA
Q5JEW2 and Q5JIA9
pH 6.5, 55C
additional information
2-(imidazol-4-yl)acetate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
37.9
ATP
Thermococcus kodakarensis
Q5JEW2 and Q5JIA9
pH 6.5, 55C
39.6
CoA
Thermococcus kodakarensis
Q5JEW2 and Q5JIA9
pH 6.5, 55C
additional information
2-(imidazol-4-yl)acetate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1260
ATP
Thermococcus kodakarensis
Q5JEW2 and Q5JIA9
pH 6.5, 55C
4
633
CoA
Thermococcus kodakarensis
Q5JEW2 and Q5JIA9
pH 6.5, 55C
18
additional information
2-(imidazol-4-yl)acetate
29157
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
Q5JEW2 and Q5JIA9
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
Q5JEW2 and Q5JIA9
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27148
Q5JEW2 and Q5JIA9
2 * 47645 + 2 * 27148, calculated from sequence
47645
Q5JEW2 and Q5JIA9
2 * 47645 + 2 * 27148, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Q5JEW2 and Q5JIA9
2 * 47645 + 2 * 27148, calculated from sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
Q5JEW2 and Q5JIA9
half-life of the TK2127/TK0943 protein: 140 min
90
Q5JEW2 and Q5JIA9
half-life of the TK2127/TK0943 protein: 120 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant TK2127/TK0943 protein
Q5JEW2 and Q5JIA9
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coexpression with gene TK0943 in Escherichia coli
Q5JEW2 and Q5JIA9