Information on EC 6.2.1.9 - malate-CoA ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.2.1.9
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RECOMMENDED NAME
GeneOntology No.
malate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + malate + CoA = ADP + phosphate + malyl-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ethylmalonyl-CoA pathway
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formaldehyde assimilation I (serine pathway)
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Glyoxylate and dicarboxylate metabolism
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Methane metabolism
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Microbial metabolism in diverse environments
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ethylmalonyl-CoA pathway
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SYSTEMATIC NAME
IUBMB Comments
malate:CoA ligase (ADP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37318-58-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PerGlx1
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Manually annotated by BRENDA team
Bacteria PerGlx1
strain PerGlx1
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Manually annotated by BRENDA team
Methanogenic bacterium
coculture of Methanospirillum hungatii strain SK, Acetobacterium woodii, and Desulfovibrio vulgaris strain Marburg
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Manually annotated by BRENDA team
strain AM 1
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Manually annotated by BRENDA team
strain MA
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-mercaptosuccinate + CoA
ADP + phosphate + 2-mercaptosuccinyl-CoA
show the reaction diagram
ATP + DL-isocitrate + CoA
ADP + phosphate + isocitryl-CoA
show the reaction diagram
ATP + malate + CoA
?
show the reaction diagram
ATP + malate + CoA
ADP + phosphate + malyl-CoA
show the reaction diagram
ATP + succinate + CoA
ADP + phosphate + succinyl-CoA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + malate + CoA
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chromium-ATP
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dead-end inhibitor
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DTNB
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Methoxycarbonyl-CoA disulfide
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ATP protects, low concentrations of succinyl-CoA protect, L-malate protects only in absence of added sulfate. The inhibitor produces half-of-the-sites reactivity with respect to enzyme phosphorylation
p-hydroxymercuribenzoate
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phosphate
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product inhibitor
succinyl-CoA
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product inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
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2 * 34000 (alpha) + 2 * 42500 (beta), SDS-PAGE, the phosphoenzyme has an alpha2beta2 structure
42500
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2 * 34000 (alpha) + 2 * 42500 (beta), SDS-PAGE, the phosphoenzyme has an alpha2beta2 structure
255000
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gel filtration, nonphosphorylated enzyme, in presence of 20% sucrose
290000
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gel filtration
300000
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gel filtration, nonphosphorylated enzyme, in absence of sucrose
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the phosphorylated enzyme is acid labile and base stable
854
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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70% loss of activity after 10 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
during purification and storage, the enzyme preparation is supplemented with 50 mM KCl and 1 mM DTT to achieve an optimal stabilization
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rapidly denatured when frozen and thawed one time
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for several weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE