Information on EC 6.2.1.8 - oxalate-CoA ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.2.1.8
-
RECOMMENDED NAME
GeneOntology No.
oxalate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + oxalate + CoA = AMP + diphosphate + oxalyl-CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
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-
Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
oxalate:CoA ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
37318-57-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Cajanus indicus
-
-
-
Manually annotated by BRENDA team
chick-pea
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain P-24
-
-
Manually annotated by BRENDA team
Lens sp.
lentil
-
-
Manually annotated by BRENDA team
Lupinus sp.
-
-
-
Manually annotated by BRENDA team
Pumpkin
-
-
-
Manually annotated by BRENDA team
gene YBR222C
UniProt
Manually annotated by BRENDA team
gene YBR222C
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
the enzyme catalyzes the first step in oxalate degradation
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + oxalate + CoA
?
show the reaction diagram
ATP + oxalate + CoA
AMP + diphosphate + oxalyl-CoA
show the reaction diagram
CTP + oxalate + CoA
CMP + diphosphate + oxalyl-CoA
show the reaction diagram
-
at 15% the activity relative to ATP
-
-
-
GTP + oxalate + CoA
GMP + diphosphate + oxalyl-CoA
show the reaction diagram
-
at 6% of the actvity relative to ATP
-
-
-
UTP + oxalate + CoA
UMP + diphosphate + oxalyl-CoA
show the reaction diagram
-
at 21% the activity relative to ATP
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + oxalate + CoA
?
show the reaction diagram
ATP + oxalate + CoA
AMP + diphosphate + oxalyl-CoA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
-
inhibition of ATP-diphosphate exchange
MgCl2
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high concentrations cause a marked inhibition of ATP-diphosphate exchange
NaF
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slight inhibition of ATP-diphosphate exchange
oxalate
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high concentrations cause a slight inhibition of ATP-diphosphate exchange
PCMB
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inhibition of ATP-diphosphate exchange. Reversal of inhibition by GSH or cysteine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
ATP
-
-
0.1
CoA
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-
0.02 - 2
oxalate
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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ATP-diphosphate exchange
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
activity range, profile overview
6 - 10
activity range, profile overview
6.3 - 8.6
-
6.3: about 60% of maximal activity, 8.6: about 50% of maximal activity, ATP-diphosphate exchange
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3 enzyme forms: OCS-1, OCS-2 and OCS-3
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recombinant His-tagged enzyme from Escherichia coli strain BLR(DE3) by nickel affinity chromatography to over 90% purity
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm
gene YBR222C, expression of His-tagged enzyme in Escherichia coli strain BLR(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
oxalate induced the enzyme, quantitative RT-PCR reveals that ScAAE3 transcript levels are induced within min of exposure peaking at about 1 h before declining back to steady state levels
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information