Information on EC 6.2.1.42 - 3-oxocholest-4-en-26-oate-CoA ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.2.1.42
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RECOMMENDED NAME
GeneOntology No.
3-oxocholest-4-en-26-oate-CoA ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cholesterol degradation to androstenedione I (cholesterol oxidase)
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cholesterol degradation to androstenedione II (cholesterol dehydrogenase)
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SYSTEMATIC NAME
IUBMB Comments
3-oxocholest-4-en-26-oate:CoA ligase (AMP-forming)
The enzyme, characterized from actinobacterium Mycobacterium tuberculosis, catalyses a step in the degradation of cholesterol. It is responsible for the activation of the C8 side chain. 3beta-hydroxycholest-5-en-26-oate can also be used as substrate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-hydroxy-5-cholesten-26-oate
?
show the reaction diagram
ATP + 3-oxocholest-4-en-26-oate + CoA
AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
show the reaction diagram
ATP + 5-cholenic acid-3beta-ol
?
show the reaction diagram
ATP + 5-cholestene-26-oic acid-3beta-ol
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 3-oxocholest-4-en-26-oate + CoA
AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
show the reaction diagram
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
33
3-oxocholest-4-en-26-oate
Mycobacterium tuberculosis
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at pH 7.3 and 22°C
193513
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli Rosetta 2(DE3)/pLysS cells
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