Information on EC 6.2.1.39 - [butirosin acyl-carrier protein]-L-glutamate ligase

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The expected taxonomic range for this enzyme is: Bacillus circulans

EC NUMBER
COMMENTARY hide
6.2.1.39
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RECOMMENDED NAME
GeneOntology No.
[butirosin acyl-carrier protein]-L-glutamate ligase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] = ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
show the reaction diagram
(2)
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ATP + L-glutamate + BtrI acyl-carrier protein = ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
show the reaction diagram
(1)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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butirosin biosynthesis
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Neomycin, kanamycin and gentamicin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
[BtrI acyl-carrier protein]:L-glutamate ligase (ADP-forming)
Catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family. The enzyme adds one molecule of L-glutamate to a dedicated acyl-carrier protein, and following decarboxylation of the product by EC 4.1.1.95, L-glutamyl-[BtrI acyl-carrier protein] decarboxylase, adds a second L-glutamate molecule. Requires Mg2+ or Mn2+, and activity is enhanced in the presence of Mn2+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene btrJ
UniProt
Manually annotated by BRENDA team
gene btrJ
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 4-aminobutanoate + BtrI acyl-carrier protein
ADP + phosphate + 4-aminobutanoyl-S-[BtrI acyl-carrier protein]
show the reaction diagram
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
show the reaction diagram
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + L-Glu-4-aminobutanoyl-S-[BtrI acyl-carrier protein]
show the reaction diagram
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-glutamate + 4-aminobutanoyl-[BtrI acyl-carrier protein]
ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
show the reaction diagram
ATP + L-glutamate + BtrI acyl-carrier protein
ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
essentially required
Mn2+
essentially required, activates
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48600
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50962
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
50969
1 * 48600, about, SDS-PAGE, 1 * 50962, His6-tagged BtrJ, mass spectrometry, 1 * 50969, His6-tagged BtrJ, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene btrJ, butirosin genetic cluster organization, overview. Expression of N-terminal His6-tagged BtrJ