Information on EC 6.2.1.35 - ACP-SH:acetate ligase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.2.1.35
-
RECOMMENDED NAME
GeneOntology No.
ACP-SH:acetate ligase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + acetate + an [acyl-carrier protein] = AMP + diphosphate + an acetyl-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
malonate degradation II (biotin-dependent)
-
-
SYSTEMATIC NAME
IUBMB Comments
acetate:[acyl-carrier-protein] ligase (AMP-forming)
This enzyme, from the anaerobic bacterium Malonomonas rubra, is a component of the multienzyme complex EC 4.1.1.89, biotin-dependent malonate decarboxylase. The enzyme uses the energy from hydrolysis of ATP to convert the thiol group of the acyl-carrier-protein-bound 2'-(5-phosphoribosyl)-3'-dephospho-CoA prosthetic group into its acetyl thioester [2].
CAS REGISTRY NUMBER
COMMENTARY hide
80700-20-5
multienzyme complex malonate decarboxylase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-carrier protein + acetate + ATP
acetyl-acyl carrier protein + AMP + diphosphate
show the reaction diagram
-
-
acyl carrier protein contains 2-(5-phosphoribosyl)-3-dephosphocoenzyme A as a prosthetic group
-
?
additional information
?
-
-
catalytic mechanism of malonate decarboxylase and involvement of ACP-SH acetate:ligase
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
not inhibitory: dithioerythritol, iodoacetate
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
during purification of the malonate decarboxylase complex, the ligase is separated from the decarboxylase by treatment with 1.5 M ammonium sulfate after the TSK-DEAE column
-